Pancreatic Islets from Non-Heart-Beating Donor Pig: Two-layer Preservation Method in an in Vitro Porcine Model

Gioviale, Maria Concetta; Damiano, Giuseppe; Palumbo, Vincenzo Davide; Bellavia, Maurizio; Cacciabaudo, Francesco; Cassata, Giovanni; Puleio, Roberto; Altomare, Roberta; Monte, Attilio Ignazio Lo

doi:10.5301/ijao.2011.8465

Cited by 9 publications

(7 citation statements)

References 26 publications

(23 reference statements)

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“…Moreover, previous studies have shown that oxygenation through preservation with the TLM (UW/PFC solution) reduces cold ischemic damage even when organs are collected from non-heart-beating donors. 10 Thus a combination of methods might improve pancreas preservation for short CIT, increasing cell viability and, as a consequence, extending the effective duration of preservation. This improvement might extend the donor pool to marginal organs and improve transplant outcomes.…”

Section: Discussionmentioning

confidence: 99%

“…8 Moreover we have demonstrated that ischemic injury can be minimized by static oxygenation of tissue during cold ischemia by two-layer preservation in University of Wisconsin (UW)/perfluorocarbons solution (twolayer method). 9,10 Previously collection had been optimized utilizing fluorescence cell sorting to collect purified ␤-cell precursors from whole human adult and children pan-creata. 11,12 The objective of this investigation was to determine the histologic impact of TLM compared with classic UW preservation on pancreatic tissue recovery and as secondarily to demonstrate whether TLM perfusion increased the number of viable exocrine cells from rat pancreas after in situ perfusion and 8 weeks of cryopreservation.…”

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confidence: 99%

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Histologic Effects of University of Wisconsin Two-Layer Method Preservation of Rat Pancreas

Gioviale

Damiano

Puleio

et al. 2013

Transplantation Proceedings

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Marginal donors represent a poorly utilized source of organs for transplantation despite their availability. The key is to reduce the ischemic damage in the effort to improve organ quality. This study investigated the histologic effects after in situ perfusion of preservation with a two-layer method compared with the classic University of Wisconsin preservation in term of tissue integrity and number of viable exocrine cells in the rat pancreas both after exsanguination and at 8 weeks of cryopreservation. Pancreata harvested from 60 rats were collected using 3 methods: two-layer method following University of Wisconsin perfusion; exsanguination; and classic University of Wisconsin perfusion/storage. In addition to histologic analysis of collected pancreata, we analyzed the number of CK19(+) cells and their viability using chi-square tests with values P < .05 considered to be significant. Rat pancreas histology showed as University of Wisconsin in situ perfusion and preservation by the two-layer method to be more effective to maintain the morphologic integrity of both exocrine and endocrine tissues. There were a larger number of CK19(+) cells with good viability. Moreover, the effects of oxygenation were visible in pancreas biopsies preserved after exsanguination. In situ University of Wisconsin perfusion and preservation for 240 minutes with the two-layer method yielded greater numbers and viability of CK19(+) cells even after 8 weeks of cryopreservation.

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Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

Histologic Effects of University of Wisconsin Two-Layer Method Preservation of Rat Pancreas

Gioviale

Damiano

Puleio

et al. 2013

Transplantation Proceedings

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“…Mesenchymal stem cells are easily isolated from the donor, can be expanded in culture maintaining genetic stability, are little immunogenic and thus suitable for allogeneic transplants 36,37 . The potential of mesenchymal stem cells goes beyond the conventional differentiation in different cell lines and it includes the mesodermal cells differentiation into liver, kidney, muscle, epithelial, neuronal and cardiac cells 38,39 . More than 120 clinical trials are in progress, using stem cells for different therapeutic applications.…”

Section: Possible Therapeutic Applications Of Mscsmentioning

confidence: 99%

Towards an ideal source of mesenchymal stem cell isolation for possible therapeutic application in regenerative medicine

Bellavia¹,

Altomare²,

Cacciabaudo³

et al. 2014

Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub

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“…Obviously, islet isolation 47 breaks vascularization, which can compromise irremediably the endocrine function; a preservation method needs to counteract this deleterious effect. 48,49 Endothelial cells deliver oxygen and nutrients to endocrine cells, contributing to create a microenvironment for ␤-cell function. In particular, they can induce insulin gene expression during islet development, stimulate ␤-cell proliferation, and produce a number of vasoactive, angiogenic substances, and growth factors.…”

Section: Cooperation Between ␤-Cells and Bone Marrow: Stem Cells As Amentioning

confidence: 99%

Beyond Islet Transplantation in Diabetes Cell Therapy: From Embryonic Stem Cells to Transdifferentiation of Adult Cells

Gioviale

Bellavia

Damiano

et al. 2013

Transplantation Proceedings

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Exogenous insulin is, at the moment, the therapy of choice of diabetes, but does not allow tight regulation of glucose leading to long-term complications. Recently, pancreatic islet transplantation to reconstitute insulin-producing β cells, has emerged as an alternative promising therapeutic approach. Unfortunately, the number of donor islets is too low compared with the high number of patients needing a transplantation leading to a search for renewable sources of high-quality β-cells. This review, summarizes more recent promising approaches to the generation of new β-cells from embryonic stem cells for transdifferentiation of adult cells, particularly a critical examination of the seminal work by Lumelsky et al.

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Pancreatic Islets from Non-Heart-Beating Donor Pig: Two-layer Preservation Method in an in Vitro Porcine Model

Cited by 9 publications

References 26 publications

Histologic Effects of University of Wisconsin Two-Layer Method Preservation of Rat Pancreas

Histologic Effects of University of Wisconsin Two-Layer Method Preservation of Rat Pancreas

Towards an ideal source of mesenchymal stem cell isolation for possible therapeutic application in regenerative medicine

Beyond Islet Transplantation in Diabetes Cell Therapy: From Embryonic Stem Cells to Transdifferentiation of Adult Cells

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