Pancreatic lipase‐related protein 2 is essential for egg hatching in the brown planthopper, Nilaparvata lugens

Abstract

In this study, we identified a pancreatic lipase-related protein 2 (PLRP2) gene through searching the transcriptome data of the brown planthopper, Nilaparvata lugens, a monophagous rice pest. PLRP2 mRNAs were first isolated in the human pancreas and play an important role in hydrolysis of galactolipids from the diet. Although homologous PLRP2 genes have been identified in many insect species, their physiological functions remain unknown. The present study for the first time reports the functional role of PLRP2 in an insect species. Differing from mammal PLRP2s, N. lugens PLRP2 was highly expressed in developing oocytes of the ovaries of female adults and little expressed in laid eggs. Suppression of N. lugens PLRP2 expression using RNA interference significantly inhibited egg hatching in rice seedlings, implying that N. lugens PLRP2 was important for oocyte maturation and development. This novel finding will improve our understanding of the reproductive strategies in insects and provides a potential target for future management of crop pests.

A Novel Digestive Proteinase Lipase Member H-A in Bombyx mori Contributes to Digestive Juice Antiviral Activity against B. mori Nucleopolyhedrovirus

“…Lipases play an important role in lipid metabolism and energy homeostasis because fatty acids, mostly stored as triacylglycerides (TAG), are the major endogenous source of energy [37]. Insect lipases have key roles in lipid acquisition, storage and mobilization, and they are also fundamental to many physiological processes, such as insect reproduction, development, defense against pathogens, oxidative stress and pheromone signaling [25][26][27][28]30]. Additionally, the lipase-like gene (ORF19) from Heliothis virescens ascovirus (HvAV-3e) has been shown to be essential for virus replication and cell cleavage [38].…”

“…They play a crucial role in fat metabolism by catalyzing the hydrolysis of triacylglycerol to free fatty acids and glycerol [23,24]. In insects, the lipase and lipase homages are related to survivability, reproductive capacity [25], oocyte maturation and development [26], and sex pheromone biosynthesis [27]. Additionally, the lipase-related protein mRNA in midgut showed different expression patterns after challenge with different microorganisms in the Chinese oak silkworm, Antheraea pernyi [28].…”

NADPH Oxidase 5 Is Essential for Molting and Oviposition in a Rice Planthopper Nilaparvata lugens

“…Oviposition and egg hatching experiments were carried out according to Xu et al [ 21 ] and Shen et al [ 22 ]. Briefly, the 5th-instar nymphs were microinjected with ds Nox5 and ds GFP , respectively, and were reared on fresh rice seedlings until emergence.…”

Recent advances in molecular biology research of a rice pest, the brown planthopper

“…It was found that an annexin-like protein and a salivary sheath protein are necessary for salivary sheath formation (Huang et al 2015b(Huang et al , 2016a. Several salivary proteins, including salivap3, carbonic anhydrase, and the catalase-like protein Kat-1, play key roles in the feeding behavior and survival of N. lugens on rice plants (Petrova and Smith 2015;Huang et al 2016aHuang et al , 2017. More recently, an endo-β-1,4-glucanase and an EFhand calcium-binding protein were identified to function as effectors in N. lugens saliva, mediating defense responses in rice (Ji et al 2017;Ye et al 2017).…”

“…More recently, an endo-β-1,4-glucanase and an EFhand calcium-binding protein were identified to function as effectors in N. lugens saliva, mediating defense responses in rice (Ji et al 2017;Ye et al 2017). Huang et al (2017b) found that a saliva component called mucin-like protein was essential for N. lugens virulence and host adaptation (Petrova and Smith 2015;Huang et al 2016aHuang et al , 2017; later, it was found that the protein can induce the immunity response in plants (Petrova and Smith 2015;Huang et al 2016aHuang et al , 2017 . These new findings provide insights into the evolutional adaptation of N. lugens to its unique plant host, rice.…”