Pancreatic Metabolism of Se76 Selenomethionine in Dogs

Goidsenhoven, Guy E. Van; Denk, Alan F.; Pfleger, Barbara; Knight, W. Andy

doi:10.1016/s0016-5085(19)34206-4

Cited by 14 publications

(5 citation statements)

References 29 publications

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“…Presence of the isotope has been recorded in pancreatic juice (Hansson and Blau, 1963), bile (Goidsenhoven, Denk, Pfleger, and Knight, 1967), gastric mucosa (Centi Colella and Pigorini, 1968), and has also been noted in material aspirated from the stomach during the present study. Whatever the mechanism involved allowance must be made for the presence of the isotope in extraglandular sites, particularly when reporting on abnormal scans.…”

Section: Discussionsupporting

confidence: 79%

Value of hypotonic duodenography as an adjunct to pancreatic scanning

McCarthy¹,

Kreel²,

Agnew³

et al. 1969

Gut

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LondonSUMMARY A technique has been developed which allows the position of the upper gastrointestinal tract, as determined radiographically, to be superimposed on the pancreatic photoscan, performed after an injection of 75Se-selenomethionine. Results of the technique applied to 27 'abnormal' and 23 'normal' subjects are described. The importance of the additional information gained by the combined technique is discussed in terms of normal variation and increased diagnostic yield, and the situations in which it was found most useful are described. The addition of correlated duodenography rendered scan reporting more objective in 50 % of cases.Comparisons of the usefulness of radiological and other techniques in solving specific diagnostic problems in pancreatic disease have been undertaken by a number of workers (Sullivan, Meaney, Rodriguez-Antunez, and : Kreel, 1967Eaton, Fleischli, Pollard, Nebesar, and Portsaid, 1968). The reports suggest that all the techniques have well defined 'blind spots'. Pancreatic scanning after injection of 75Se-selenomethionine (Rodriguez-Antu(nez, Filson, Sullivan, and Brown, 1966;Centi Colella and Pigorini, 1967;Brown, Sircus, Smith, Donaldson, Dymock, Falconer, and Small, 1968;Melmed, Agnew, and Bouchier, 1968) and hypotonic duodenography (Raia and Kreel, 1966;Bilbao, Frische, Dotter, and Rosch, 1967;Kreel, 1969;Martel, 1968) have proved to be simple, safe procedures particularly when 'tubeless' hypotonic duodenography is employed. Our experience is similar to that of Eaton et al (1968) and suggests that these are generally the most useful techniques employed in the diagnosis of pancreatitis and pancreatic carcinoma, though distinction between the two conditions is often difficult.A technique, described below, has been developed which allows the position of the duodenal loop, as determined by duodenography, to be superimposed on the photoscan. Using this technique a study was undertaken aimed primarily at answering the question, 'Does the addition of correlated hypotonic duodenography as a routine procedure in patients subjected to pancreatic scanning yield additional information helpful to the interpretation of the scan?'The study also allowed close examination of the scan in difficult interpretive situations. These arise when glandular uptake of the isotope is poor and not greatly in excess of background activity; when the isotope appears in abnormal sites or concentrations; or when the liver or bowel overlaps the pancreas. Under such conditions it may be difficult if not impossible to delineate precise boundaries of the gland. For such reasons one-tenth of scans are unreadable and as many more are interpreted with little confidence. The technique was therefore employed in the hope of reducing this uncertainty and it permitted close study of isotope distribution in the context of known anatomical relations of the pancreas. As no similar studies had been previously reported, interpretation of the results of the study was carried out at a communal reporting session for the first 3...

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Section: Discussionsupporting

confidence: 79%

Value of hypotonic duodenography as an adjunct to pancreatic scanning

McCarthy¹,

Kreel²,

Agnew³

et al. 1969

Gut

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“…Two hours after an intravenous injection of labelled methionine about 85% of the radioactivity lies in the protein fraction of pancreatic juice obtained by cannulation of the pancreatic duct in the cat and dog (Hansson, 1959;Goidsenhoven et al, 1967). Our preliminary experiments showing that about half the radioactivity lies in the protein fraction of the duodenal juice at 120 minutes suggest that not all the radioactivity of the duodenal aspirate originates from the pancreas.…”

Section: Discussionmentioning

confidence: 92%

“…Animal experiments indicate that the time taken to reach the peak 75Se activity in pancreatic juice lies between 120 and 240 minutes (Hansson, 1959;Hansson and Blau, 1963;Goidsenhoven et al, 1967). We have not yet determined the timing of peak radioactivity in the duodenal aspirate in man; nevertheless, our results indicate that the measurement of duodenal radioactivity at 120 or 150 minutes after the injection of 75Se-selenomethionine correlates significantly with duodenal tryptic activity and that the agreement between these two measurements was poor in very few subjects.…”

Section: Discussionmentioning

confidence: 99%

Radioselenium in Duodenal Aspirate as an Assessment of Pancreatic Exocrine Function

Youngs¹,

Agnew²,

Levin³

et al. 1971

BMJ

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“…In monkeys, 4 hr after intravenous injection of methionine-75 Se, the pancreas still contains 90% of the amount of radioactivity present 1 hr after the injection (Zuidema et al ., 1963) . In dogs, only 7 % of the amount of radio-activity present in the gland 2 hr after intravenous injection of methionine-75Se is secreted during the two subsequent hours (Goidsenhoven et al ., 1967) .…”

Section: Discussionmentioning

confidence: 99%

Unstimulated Secretion of Protein From Rat Exocrine Pancreas Cells

Kramer

Poort

1972

The Journal of Cell Biology

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Our earlier work demonstrated that the rate of protein synthesis in the exocrine cells of the rat pancreas is constant in different physiological states, including prolonged fasting . In this study we have followed the fate of the protein in the pancreatic cells of the fasting animal in vivo as well as in vitro . The data were obtained by quantitative radioautography and by biochemical determinations . In nonanesthesized, fasting rats, without cannulated pancreatic duct, some 80% of the proteins synthesized at a given time leaves the cell within 12 hr by way of secretion, intracellular breakdown not being important . Two mechanisms of fasting secretion exist . The first, starting at a slow rate after 20 min, is inferred to result from fortuitous contacts of young secretory granules with the apical cell membrane . The rate of secretion is the same in vivo as in vitro, at least during the first 4 hr after pulse labeling. Within 7 hr about 20% of the total amount of newly synthesized protein has left the cell . The second mechanism consists of an orderly movement of the mass of secretory granules towards the apical cell membrane as caused by the continuous assembly of new granules. The granules that come into contact with the cell membrane are discharged . It takes about 7-12 hr for secretory protein transported in this way to reach the cell membrane . The addition of new secretory granules to those present is essential for the second mechanism, for the blockade of protein synthesis by cycloheximide decreases the rate of this phase of secretion without interfering with the secretory process proper . Atropin does not inhibit the fasting secretion in vitro, nor does extensive washing of the tissue slices, excluding possible secretagogues as important factors in fasting secretion .

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Pancreatic Metabolism of Se76 Selenomethionine in Dogs

Cited by 14 publications

References 29 publications

Value of hypotonic duodenography as an adjunct to pancreatic scanning

Value of hypotonic duodenography as an adjunct to pancreatic scanning

Radioselenium in Duodenal Aspirate as an Assessment of Pancreatic Exocrine Function

Unstimulated Secretion of Protein From Rat Exocrine Pancreas Cells

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