Pancreatic α-amylase and lipase inhibitory activity of polyphenolic compounds present in the extract of black chokeberry (Aronia melanocarpa L.)

Comparison of in vitro anti-lipase and antioxidant activities, and composition of commercial chokeberry juices

“…The major low molecular phenolic compounds of chokeberry juice were chlorogenic acids such as 3-and 5-O-caffeoylquinic acid. The results published by Worsztynowicz et al [13] used p-nitrophenol palmitate as substrate demonstrated that chlorogenic acids and anthocyanins, except for cyanidin 3-glucoside, isolated from chokeberry fruit did not inhibit the pancreatic lipase. Unfortunately, authors of the above paper have not determined proanthocyanidins content in the extracts tested.…”

“…Reduced absorption of dietary fat from the intestine by the inhibition of digestive lipases, especially pancreatic lipase, is an approach to decreasing the risk of obesity [15]. The presence of pancreatic lipase inhibitors has recently been reported in some edible fruits, including black chokeberry [12,13,21,[25][26][27][28][29]. Inhibition of this enzyme by fruits is attributed to the phenolic compounds, such as proanthocyanidins [12,21,27], ellagitannins [25,26], anthocyanins [13,28], and flavones [29].…”

“…Black chokeberry constituents may help in reducing dietary fat absorption by inhibiting digestive lipases activity. Pancreatic lipase inhibitory activity of black chokeberry extracts has been shown in in vitro studies using simple, different p-nitrophenyl esters or 4-methylumbelliferyl oleate as substrates [12,13]. The cited studies have shown anti-lipase activity dependence on the method of lipase activity determination [12] and on type of extraction agent [13].…”

Interactions of digestive enzymes and milk proteins with tea catechins at gastric and intestinal pH

“…The inhibition of digestive enzymes might be explained by the formation of insoluble complexes of tea catechins with proteins in the gastrointestinal tract (He et al, 2007). This inevitably results in changes to the enzyme's molecular configuration and thus to the loss of its catalytic activity (Mcdougall et al, 2009;Zhang et al, 2010;Worsztynowicz et al, 2014). Some researchers have reported that the interaction between milk proteins and tea catechins promoted the absorption of catechins, which increased the bioavailability of catechins (Green et al, 2007;Xie et al, 2013), but reduced the antioxidant activity and digestibility of milk proteins during in vitro digestion (Stojadinovic et al, 2013;He et al, 2015c).…”

Polyphenols: Food Sources and Health Benefits

“…A survey conducted by Basu et al [24] showed that using a freeze-dried blueberry beverage in obese people with metabolic syndrome for 8 weeks decreased blood pressure and the concentrations of oxidized LDL cholesterol and products of lipid peroxidation. Some researchers suggested that polyphenols may inhibit lipase activity and consequently reduce lipid absorption [25,26]. Uchiyama et al [27] have shown that black tea polyphenols in rats with diet-induced obesity can inhibit intestinal lipase activity and suppress the increase of triglyceride levels.…”