Pancreatic α-Amylase Controls Glucose Assimilation by Duodenal Retrieval through N-Glycan-specific Binding, Endocytosis, and Degradation

Date, Kimie; Satoh, Ayano; Iida, Kaoruko; Ogawa, Haruko

doi:10.1074/jbc.m114.594937

Cited by 45 publications

(39 citation statements)

References 32 publications

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“…Alpha-amylase activities of rats in the diabetic control groups increased significantly (p≤0.05) when compared to those in the normal group which is consistent with the reports of Date et al, [26] showing that induction of diabetes caused damage on the pancreas leading to the release of α-amylase in pancreatic juice thereby metabolizing carbohydrate (maltose) to glucose resulting to rise in blood glucose level. Treatment with aqueous extract of Citrullus lanatus seeds significantly (p≤0.05) reduced α-amylase activities in all the treated groups.…”

Section: Discussionsupporting

confidence: 89%

Effect of Aqueous Extract of Citrullus lanatus (Water Melon) Seeds on Alloxan Induced - Diabetic Wistar Rats

Ogbeifun

Peters

Monanu

2020

AJRB

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This study was designed to evaluate the effect of aqueous extract of Citrullus lanatus (water melon) seeds on alloxan induced-diabetic wistar rats. The Wistar rats were grouped into 11 of 3 rats in each. Groups 2-11 received single dose of 120 mg/kg b.wt of alloxan monohydrate intraperitoneally. Groups 4 and 5 orally received a dose of 100 mg/kg b.wt of metformin for 7 and 21 days respectively. Groups 6, 8 and 10 orally received 200 mg/kg b.wt, 400 mg/kg b.wt, and 600 mg/kg b.wt. of the extract respectively for 7 days while groups 7, 9 and 11 orally received 200 mg/kg b.wt., 400 mg/kg b.wt and 600 mg/kg b.wt of the extract respectively for 21 days. Blood samples were collected 24 hours after 7 and 21 days of treatment and selected biochemical parameters were determined; glucose determination, pancreatic α- amylase activities and lipid profile, while histopathology examination of the pancreas was also examined. Qualitative phytochemical result showed the presence of flavonoids, alkaloids, saponin, tannin, oxalate and phytate. Flavonoids (176.87 ug/g) were most abundant and phytate was the least occurring anti nutrient (6.75±0.06). Citrullus lanatus seeds extract significantly (p≤0.05) decreased the plasma glucose levels, pancreatic α-amylase activities, total cholesterol, triglycerides and lipoproteins while there was a significant (p≤0.05) increase in high density lipoproprotein levels on 7 and 21 days in all the extract treated groups. The histopathological examination of the pancreas showed regeneration of pancreatic cells in all the extract treated groups. The aqueous extract of Citrullus lanatus (water melon) seeds is rich in phytochemicals with antioxidant properties hence could serve as novel herbal antidiabetic agent.

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Section: Discussionsupporting

confidence: 89%

Effect of Aqueous Extract of Citrullus lanatus (Water Melon) Seeds on Alloxan Induced - Diabetic Wistar Rats

Ogbeifun

Peters

Monanu

2020

AJRB

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“…Salivary amylase passing through the stomach in substrate bound form comes into contact with a very low level of pancreatic enzyme proteolytic activity in the intestine of bariatric pigs and thus it is not destroyed. This hypothesis is supported by recent experiments by Date et al 55 , 56 as well as by some earlier findings 65 .…”

Section: Pancreatic Enzymes In Glycaemic Controlsupporting

confidence: 79%

“…Taking into account other studies where reduced blood amylase concentrations were associated with glucose intolerance, we speculate that the mechanism behind these findings is based on the specific signal transduction of enteral or parenteral amylase or amylase-derived components/peptides, which interact with the glycoconjugates—possibly receptors on the apical or basolateral surface of the enterocyte 48 . Pancreatic amylase has been reported to bind to N -glycans in intestinal brush border membrane and inhibit intestinal glucose absorption by SGLT1 55 , 56 . Furthermore, amylase and/or amylase derivatives could enhance glucose uptake in insulin-independent tissues such as enterocytes via GLUT1 or GLUT2, and in this case could serve as a defensive mechanism against the development of hyperglycemia.…”

Section: Pancreatic Enzymes In Glycaemic Controlmentioning

confidence: 99%

Glucose homeostasis dependency on acini–islet–acinar (AIA) axis communication: a new possible pathophysiological hypothesis regarding diabetes mellitus

Pierzynowski

Gregory²,

Filip

et al. 2018

Nutr & Diabetes

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Studies have highlighted the existence of two intra-pancreatic axes of communication: one involved in the regulation of enzyme production by insulin—the insular–acinar axis; and another involved in the regulation of insulin release by pancreatic enzymes—the acini–insular axis. Previous studies by our laboratory show that pancreatic enzymes can affect blood glucose homeostasis and insulin secretion independently of their digestive functions, both from the gut lumen and probably from the blood. As a result we would like to introduce here the concept of acini–islet–acinar (AIA) axis communication (feedback), which could play an important role in the development of obesity and diabetes type 2. The AIA feedback links the endocrine and exocrine parts of the pancreas and emphasizes the essential role that the pancreas plays, as a single organ, in the regulation of glucose homeostasis by amylase most probably in gut epithelium and by insulin and glucagon in peripheral blood.

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“…In animals, α-amylases (α-1,4 glucan-4-gluconohydrolase, EC 3.2.1.1) have a central role in the initial steps of the digestion of key glycogenic substrates such as dietary starch and dietary and/or storage glycogen (Singh and Kayastha, 2014;Date et al, 2015). However, in spite of their inherent physiological importance, the current knowledge about forms, modulation by environmental factors, and biochemical features of amylase in the hepatopancreas (the main site of digestive enzyme synthesis and of digestion and absorption of nutrients and storage of energy substrates) of groups of ecological importance such as intertidal euryhaline crabs is still scarce and fragmentary (Blandamer and Beechey, 1966;Van Wormhoudt et al, 1995;Asaro et al, 2011;Zeng et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

Amylase in the hepatopancreas of a euryhaline burrowingcrab: characteristics and modulation

Asaro¹,

Paggi²,

Castro³

et al. 2017

Turk J Zool

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In spite of its inherent physiological importance, studies on the occurrence, characteristics, and modulation of amylase in euryhaline crabs are lacking. We investigated the occurrence of amylase forms and the effect of acclimation to different salinities on their number and made a partial purification and characterization of the major form present in the hepatopancreas of Neohelice granulata. Zymogram analysis revealed 5 amylase forms in crabs acclimated to 35 psu (seawater) and 37 psu, and an additional band at 10 psu, but with a major form (29 kDa) in all cases, which was partially purified and characterized. Amylolytic activity was maximal between 30 and 40 °C; maintained at high NaCl concentrations (up to 4 M); increased by 5 mM K + , Li + , Co 2+ , and Mg 2+ (36%-45%); inhibited by Cu 2+ , Zn 2+ , Cd 2+ , Fe 2+ , and Mn 2+ (92.4% and 23.7%); not affected by Ni 2+ or Ba 2+ ; and enhanced almost 100% by Ca 2+ . Amylase exhibited Michaelis-Menten kinetics (starch: K m = 1.24 mg mL -1 ; glycogen: K m = 16.19 mg mL -1 ). The potential physiological significance and relationship to habitat conditions of the extra form in low salinity and the biochemical characteristics of the partially purified amylolytic activity (halotolerant, differential sensitivity to ions, capability to hydrolyze starch and glycogen) are discussed.

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Pancreatic α-Amylase Controls Glucose Assimilation by Duodenal Retrieval through N-Glycan-specific Binding, Endocytosis, and Degradation

Cited by 45 publications

References 32 publications

Effect of Aqueous Extract of Citrullus lanatus (Water Melon) Seeds on Alloxan Induced - Diabetic Wistar Rats

Effect of Aqueous Extract of Citrullus lanatus (Water Melon) Seeds on Alloxan Induced - Diabetic Wistar Rats

Glucose homeostasis dependency on acini–islet–acinar (AIA) axis communication: a new possible pathophysiological hypothesis regarding diabetes mellitus

Amylase in the hepatopancreas of a euryhaline burrowingcrab: characteristics and modulation

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