Pancreaticoduodenal Graft in the Rat: An Original Microsurgical Technique

Abstract

The authors describe an original surgical technique for pancreatic transplantation carried out on an experimental rat model which duplicates as closely as possible the anatomic-functional situation of a pancreatic graft in man (one arterovenous vascular peduncle with one functioning kidney only). The technique entails a microanastomosis between the mesenteric artery and the portal vein of the donor organ with the left renal artery and vein of the recipient. This method appears to be technically encouraging and particularly useful in studying the early phases of ischemic graft injuries, a field in which the authors are particularly interested.

Ischemia/reperfusion-induced pancreatitis in rats: a new model of complete normothermic in situ ischemia of a pancreatic tail-segment

“…Because ex vivo reperfused models [23] show no physiological regulation of perfusion, they do not seem to be suitable for microcirculatory investigations. In models of pancreatic transplantation [24], immunological reactions can also influence the changes induced by ischemia/reperfusion injury. Models of in situ ischemia/reperfusion of the pancreas where the ischemia is induced by clamping only the main supplying vessels [19,25] do not allow flushing of the pancreatic tail-segment before ischemia.…”

Surgical Morbidity of Simultaneous Kidney and Pancreas Transplantation: A Single-Centre Experience in the Tacrolimus Era

“…e �ve-year patient, pancreas, and kidney survival aer SPK transplantation in this series are 94%, 77%, and 89%, respectively. is compares favourably with United States data in which �ve-year patient and pancreas-gra survival rates were 87% and 71-73%, respectively, for patients who received a SPK transplant [26,27].…”

Microsurgical Technique in Simultaneous Pancreas-/Kidney Transplantation of the Rat – Clinical Experience and Review of Literature

“…Continuing research in microsurgery has considerably expanded experimental surgery and given new impulses to clinical surgery [1][2][3][4] . Whole-organ pancreas transplantation in the rat was first described by Lee et al [3,4] in 1972.…”

“…Simultaneous en bloc pancreaticoduodenal kidney transplantation is being applied with increasing frequency in the treatment of diabetes mellitus type I and selected cases of type II [5][6][7][8] . A review of the literature of isolated pancreas transplantation in rat models showed that different techniques or modifications can be applied [2,4,9,[11][12][13] . However, pancreas transplantation in the Key Words Transplantation ؒ Pancreas ؒ Kidney ؒ Allograft ؒ Surgical technique ؒ Rat model Abstract Background: For experimental basic research, standardized transplantation models reflecting technical and immunologic aspects are necessary.…”