Emergence and re-emergence
of pathogens bearing the risk of becoming
a pandemic threat are on the rise. Increased travel and trade, growing
population density, changes in urbanization, and climate have a critical
impact on infectious disease spread. Currently, the world is confronted
with the emergence of a novel coronavirus SARS-CoV-2, responsible
for yet more than 800 000 deaths globally. Outbreaks caused
by viruses, such as SARS-CoV-2, HIV, Ebola, influenza, and Zika, have
increased over the past decade, underlining the need for a rapid development
of diagnostics and vaccines. Hence, the rational identification of
biomarkers for diagnostic measures on the one hand, and antigenic
targets for vaccine development on the other, are of utmost importance.
Peptide microarrays can display large numbers of putative target proteins
translated into overlapping linear (and cyclic) peptides for a multiplexed,
high-throughput antibody analysis. This enabled for example the identification
of discriminant/diagnostic epitopes in Zika or influenza and mapping
epitope evolution in natural infections versus vaccinations. In this
review, we highlight synthesis platforms that facilitate fast and
flexible generation of high-density peptide microarrays. We further
outline the multifaceted applications of these peptide array platforms
for the development of serological tests and vaccines to quickly encounter
pandemic threats.