Pandrug-resistant Pseudomonas sp. expresses New Delhi metallo-β-lactamase-1 and consumes ampicillin as sole carbon source

Pseudomonas aeruginosa is the most frequent cause of infection among non-fermenting Gram-negative bacteria, predominantly affecting immunocompromised patients, but its pathogenic role should not be disregarded in immunocompetent patients. These pathogens present a concerning therapeutic challenge to clinicians, both in community and in hospital settings, due to their increasing prevalence of resistance, and this may lead to prolonged therapy, sequelae, and excess mortality in the affected patient population. The resistance mechanisms of P. aeruginosa may be classified into intrinsic and acquired resistance mechanisms. These mechanisms lead to occurrence of resistant strains against important antibiotics—relevant in the treatment of P. aeruginosa infections—such as β-lactams, quinolones, aminoglycosides, and colistin. The occurrence of a specific resistotype of P. aeruginosa, namely the emergence of carbapenem-resistant but cephalosporin-susceptible (Car-R/Ceph-S) strains, has received substantial attention from clinical microbiologists and infection control specialists; nevertheless, the available literature on this topic is still scarce. The aim of this present review paper is to provide a concise summary on the adaptability, virulence, and antibiotic resistance of P. aeruginosa to a readership of basic scientists and clinicians.

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“…Recently, a PDR strain of Pseudomonas sp. strain has been described, which was capable of using ampicillin as a sole carbon source [158].…”

Section: Antibiotic Resistance In P Aeruginosa: Therapeutic Alternatmentioning

confidence: 99%

It’s Not Easy Being Green: A Narrative Review on the Microbiology, Virulence and Therapeutic Prospects of Multidrug-Resistant Pseudomonas aeruginosa

2021

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“…To explore potential ITC‐tolerance mechanisms, we tested if ITC tolerance correlated with tolerance to ampicillin beta‐lactam antibiotic (growth assays), which is commonly produced by various soil bacteria (Ranjan et al, 2021). Moreover, we specifically tested for ampicillin tolerance as we identified potential antibiotic‐linked insertion sequence movement in our evolved clones, which has previously been shown to confer beta‐lactam antibiotic tolerance in clinical settings (Boutoille et al, 2004; Poirel et al, 2003).…”

Section: Methodsmentioning

confidence: 99%

Plant pathogenic bacterium can rapidly evolve tolerance to an antimicrobial plant allelochemical

Greenrod

Friman

2022

Evolutionary Applications

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Crop losses to plant pathogens are a growing threat to global food security and more effective control strategies are urgently required. Biofumigation, an agricultural technique where Brassica plant tissues are mulched into soils to release antimicrobial plant allelochemicals called isothiocyanates (ITCs), has been proposed as an environmentally friendly alternative to agrochemicals. Whilst biofumigation has been shown to suppress a range of plant pathogens, its effects on plant pathogenic bacteria remain largely unexplored. Here, we used a laboratory model system to compare the efficacy of different types of ITCs against Ralstonia solanacearum plant bacterial pathogen. Additionally, we evaluated the potential for ITC‐tolerance evolution under high, intermediate, and low transfer frequency ITC exposure treatments. We found that allyl‐ITC was the most efficient compound at suppressing R. solanacearum growth, and its efficacy was not improved when combined with other types of ITCs. Despite consistent pathogen growth suppression, ITC tolerance evolution was observed in the low transfer frequency exposure treatment, leading to cross‐tolerance to ampicillin beta‐lactam antibiotic. Mechanistically, tolerance was linked to insertion sequence movement at four positions in genes that were potentially associated with stress responses (H‐NS histone like protein), cell growth and competitiveness (acyltransferase), iron storage ([2‐Fe‐2S]‐binding protein) and calcium ion sequestration (calcium‐binding protein). Interestingly, pathogen adaptation to the growth media also indirectly selected for increased ITC tolerance through potential adaptations linked with metabolism and antibiotic resistance (dehydrogenase‐like protein) and transmembrane protein movement (Tat pathway signal protein). Together, our results suggest that R. solanacearum can rapidly evolve tolerance to allyl‐ITC plant allelochemical which could constrain the long‐term efficiency of biofumigation biocontrol and potentially shape pathogen evolution with plants.

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“…To explore potential ITC-tolerance mechanisms, we tested if ITC tolerance correlated with tolerance to ampicillin beta-lactam antibiotic (growth assays), which is commonly produced by various soil bacteria (51). Overall, both low (15 g/ml) and high (30 g/ml) ampicillin concentrations had negative effects on R. solanacearum growth relative to the no-ampicillin control treatment (Ampicillin concentration: F 2, 93 = 50.12, p< 0.001; Tukey: p< 0.05; high concentration relatively more inhibitory, Fig.…”

Section: (D) Evolution Of Itc-tolerance Confers Cross-tolerance To Ampicillin Betalactam Antibioticmentioning

confidence: 99%

Plant pathogenic bacterium can rapidly evolve tolerance to an antimicrobial plant allelochemical

Friman

2021

Preprint

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Crop losses to plant pathogens are a growing threat to global food security, and hence, more effective control strategies are urgently required. Biofumigation, an agricultural technique, where Brassica plant tissues are mulched into soils to release antimicrobial plant allelochemicals called isothiocyanates (ITCs), has been proposed as an environmentally friendly alternative to synthetic agrochemicals. While biofumigation has been shown to suppress a range of plant pathogens, its effects on plant pathogenic bacteria remain largely unexplored. Here we used a laboratory model system to compare the efficacy of different types of ITCs against Ralstonia solanacearum plant bacterial pathogen. Additionally, we evaluated the potential for ITC-tolerance evolution under high, intermediate and low transfer frequency ITC exposure treatments. We found that allyl-ITC was the most efficient compound at suppressing R. solanacearum growth, and its efficacy was not improved when combined with other types of ITCs. Despite consistent pathogen growth suppression, ITC tolerance evolution was observed in the low transfer frequency exposure treatment. Mechanistically, tolerance was associated with parallel mutations in a gene linked to glucose/sorbonose dehydrogenase, resulting in cross-tolerance to ampicillin beta-lactam antibiotic. Interestingly, pathogen adaptation to the growth media also indirectly selected for increased ITC tolerance through potential metabolic adaptations linked with cell wall structure (serine/threonine kinase) and DNA replication, recombination and repair (deoxyribonucleases). Together, our results suggest that R. solanacearum can rapidly evolve tolerance to allyl-ITC plant allelochemical, which could constrain the long-term efficiency of biofumigation biocontrol and potentially shape pathogen evolution with plants.

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Pandrug-resistant Pseudomonas sp. expresses New Delhi metallo-β-lactamase-1 and consumes ampicillin as sole carbon source

Cited by 6 publications

References 18 publications

It’s Not Easy Being Green: A Narrative Review on the Microbiology, Virulence and Therapeutic Prospects of Multidrug-Resistant Pseudomonas aeruginosa

It’s Not Easy Being Green: A Narrative Review on the Microbiology, Virulence and Therapeutic Prospects of Multidrug-Resistant Pseudomonas aeruginosa

Plant pathogenic bacterium can rapidly evolve tolerance to an antimicrobial plant allelochemical

Plant pathogenic bacterium can rapidly evolve tolerance to an antimicrobial plant allelochemical

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