2021
DOI: 10.4103/1673-5374.290911
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Pannexin 1, a large-pore membrane channel, contributes to hypotonicity-induced ATP release in Schwann cells

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Cited by 19 publications
(19 citation statements)
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“…In contrast, ATP release by airway epithelial cells was detectable within seconds after a sudden osmotic stimulus, was reversible, and was attenuated by the Panx1 inhibitors CBX or probenecid ( Ransford et al, 2009 ). Similarly, ATP release by Schwann cells induced by hypotonicity was detectable within 2 min after the stimulus, occurred in the absence of the cytoplasmic marker lactate dehydrogenase, and was blocked by the same Panx1 inhibitors ( Wei et al, 2021 ). Furthermore, ATP release by oocytes expressing WT Panx1 or Panx1Δ378 was detectable in the unstirred supernatant as early as 5 min after a K + stimulus ( Wang and Dahl, 2018 ).…”
Section: Activation Of Panx1 By Caspase Cleavagementioning
confidence: 99%
“…In contrast, ATP release by airway epithelial cells was detectable within seconds after a sudden osmotic stimulus, was reversible, and was attenuated by the Panx1 inhibitors CBX or probenecid ( Ransford et al, 2009 ). Similarly, ATP release by Schwann cells induced by hypotonicity was detectable within 2 min after the stimulus, occurred in the absence of the cytoplasmic marker lactate dehydrogenase, and was blocked by the same Panx1 inhibitors ( Wei et al, 2021 ). Furthermore, ATP release by oocytes expressing WT Panx1 or Panx1Δ378 was detectable in the unstirred supernatant as early as 5 min after a K + stimulus ( Wang and Dahl, 2018 ).…”
Section: Activation Of Panx1 By Caspase Cleavagementioning
confidence: 99%
“…Primary Schwann cell cultures were performed as previously described [ 18 ]. In brief, after isolation from the sciatic nerves, cells were incubated in DMEM containing 10% horse serum, and supplemented with 1 ng/ml heregulin β-1 (Peprotech, 100–03) and 0.5 μM forskolin (Sigma-Aldrich, F6886) for proliferation.…”
Section: Methodsmentioning
confidence: 99%
“…Moreover, Panx 1 activation in the dorsal root ganglion (DRG), astrocytes, and microglia participates in neuropathic pain development [ 15 17 ]. In addition, our previous study revealed that Panx 1 is highly expressed in Schwann cells compared with the other two family members and the inhibition of Panx 1 reduces hypotonicity-induced ATP release [ 18 ]. However, the contribution of Panx1 in Schwann cells regarding neuropathic pain is still unclear.…”
Section: Introductionmentioning
confidence: 99%
“…Consistently, the inhibition of Rho kinase, Ras homolog family member A (RhoA) or myosin light chain (MLC) kinase, which disrupts the actin cytoskeleton, significantly reduces ATP release via mechanosensitive PANX1 channels [38]. Further, the treatment of Schwann cells using a Rho GTPase inhibitor or small interfering RNA (siRNA) targeting Rho or cytochalasin D results in a decrease in hypotonicity-induced ATP release via PANX1 [61]. In addition, PANX1 is associated with collapsing response mediator protein 2 (Crmp2), which is a well-known microtubule-stabilizing protein [62].…”
Section: Force-from-filaments Modelmentioning
confidence: 95%