Pantoea allii sp. nov., isolated from onion plants and seed

Abstract

Eight yellow-pigmented, Gram-negative, rod-shaped, oxidase-negative, motile, facultatively anaerobic bacteria were isolated from onion seed in South Africa and from an onion plant exhibiting centre rot symptoms in the USA. The isolates were assigned to the genus Pantoea on the basis of phenotypic and biochemical tests. 16S rRNA gene sequence analysis and multilocus sequence analysis (MLSA), based on gyrB, rpoB, infB and atpD sequences, confirmed the allocation of the isolates to the genus Pantoea. MLSA further indicated that the isolates represented a novel species, which was phylogenetically most closely related to Pantoea ananatis and Pantoea stewartii. Amplified fragment length polymorphism analysis also placed the isolates into a cluster separate from P. ananatis and P. stewartii. Compared with type strains of species of the genus Pantoea that showed .97 % 16S rRNA gene sequence similarity with strain BD 390 T , the isolates exhibited 11-55 % whole-genome DNA-DNA relatedness, which confirmed the classification of the isolates in a novel species. The most useful phenotypic characteristics for the differentiation of the isolates from their closest phylogenetic neighbours are production of acid from amygdalin and utilization of adonitol and sorbitol. A novel species, Pantoea allii sp. nov., is proposed, with type strain BD 390 T (5LMG 24248 T ).

A wide diversity ofPantoealineages are engaged in mutualistic symbiosis and cospeciation processes with stinkbugs

“…We observed here the presence of novel Pantoea strains in symbiotic midgut crypts of 10 pentatomid stinkbug species. On the basis of an outstanding genetic framework using multi‐locus gene sequencing (Brady et al ., ; Brady et al ., ; Brady et al ., ; Popp et al ., ), we show that a wide genetic diversity exists beyond the 20 known Pantoea species: we evidence 10 additional Pantoea lineages, all highly differentiated and potentially constituting novel and separate Pantoea species, in diverse pentatomid stinkbug species. Although these bacteria remain to be formally described and named, some of our results, along with those of a few previous studies (Prado and Almeida, ; Bistolas et al ., ; Hosokawa et al ., ), are indicative of their remarkable biological features within the Pantoea genus.…”

“…Some Pantoea species are also consistently linked with human infections as well exemplified by Pantoea brenneri and Pantoea septica isolated only from clinical sources (Kratz et al, 2003;Schmid et al, 2003;De Baere et al, 2004;Lim et al, 2006;Van Rostenberghe et al, 2006;Fullerton et al, 2007). A substantial part of research effort in recent years has also focused on providing an accurate multi-locus typing scheme for the systematic characterisation of different Pantoea strains, leading to the identification of at least 20 distinct Pantoea species (Brady et al, 2008;Brady et al, 2009;Brady et al, 2010a;Popp et al, 2010;Brady et al, 2011;Brady et al, 2012). Nowadays, it is obvious that members of Pantoea genus are ecologically diverse, with species exhibiting a variety of niche-specific adaptations and opportunism (Walterson and Stavrinides, 2015).…”

Pantoea theicola sp. nov., isolated from black tea

“…The phenotypic characteristics of strain QC88-366 T generally corresponded to the emended description of the genus Brady et al (2008Brady et al ( , 2009Brady et al ( , 2010Brady et al ( , 2011Brady et al ( , 2012 and Popp et al (2010). Table 1.…”

“…indologenes. Data were taken from the following sources: taxa 1-3 from this study; taxa 4-8 from Brady et al (2010); taxa 9-11 from Brady et al (2012); taxa 12-15 from Brady et al (2009); taxa 16 and 17 are type strain data from Gavini et al (1989) and Grimont & Grimont (2005); taxon 18 from Brady et al (2011); taxa 19-21 are type strains data from Mergaert et al (1993).+, 90-100 % strains positive in 1-2 days; (+), slow reaction (between 2 and 30 days); 2, negative; D, 11-89 % strains positive in 1-4 days; ND, not determined. Citrate utilization 2 + + D + + + + 2 2 + 2 2 ND 2 + + + + 2 + Indol production 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 + + 2 + Acetoin production 2 + + ND ND ND ND ND + ND ND + + + + + + + + 2 + Gelatinase 2 2 + ND ND ND ND ND ND ND ND 2 2 ND 2 + + 2 2 2 2 Acid production from:…”

Effects of postharvest onion curing parameters on bulb rot caused by Pantoea agglomerans, Pantoea ananatis and Pantoea allii in storage

“…can be regarded as epiphytes (Smith et al, 2013), endophytes, plant pathogens or opportunistic human pathogens (Cruz et al, 2007). Three different species of Pantoea have been reported to be pathogenic on onion: Pantoea ananatis, Pantoea agglomerans, and the recently described Pantoea allii (Brady et al, 2011).…”