Pantoea vagans sp. nov., Pantoea eucalypti sp. nov., Pantoea deleyi sp. nov. and Pantoea anthophila sp. nov.

Abstract

Bacteria isolated from eucalyptus leaves and shoots showing symptoms of blight and die-back collected in Uganda, Uruguay and Argentina and from maize displaying brown stalk rot symptoms in South Africa were tentatively placed in the genus Pantoea on the basis of phenotypic and biochemical tests. These isolates, together with two strains (LMG 2558 and LMG 2560) previously assigned to Pantoea agglomerans based on protein electrophoregrams but later excluded from this species, were further investigated using molecular techniques. 16S rRNA gene sequencing and multilocus sequence analyses (MLSA) revealed that the strains were phylogenetically closely related to Pantoea agglomerans, Pantoea stewartii and Pantoea ananatis. P. ananatis is the causal agent of bacterial blight and dieback of eucalyptus in South Africa (Coutinho et al., 2002). In 2001, Pantoea-like strains were isolated from eucalyptus trees exhibiting a similar disease in Uganda, Argentina and Uruguay and were thought to belong to P. ananatis. P. ananatis was also found to cause brown stalk rot of maize in South Africa (Goszczynska et al., 2007). Isolated simultaneously with P. ananatis from maize were additional Pantoea-like strains which also caused brown stalk rot. Multilocus sequence analysis (MLSA) has been shown to separate the isolates from eucalyptus and maize into three clusters, indicating that these strains probably constitute three novel species of Pantoea (Brady et al., 2008). Also included in the MLSA study were two strains, LMG 2558 (5NCPPB 1682) and LMG 2560 (5NCPPB 1941), mentioned by Beji et al. (1988) as belonging to protein profiling group VII, which were assigned to P. agglomerans based on their electrophoregrams, despite never being included in DNA-DNA hybridization experiments with the type strain of P. agglomerans. It was suggested by Gavini et al. (1989) that strains of protein Abbreviations: AFLP, amplified fragment length polymorphism; MLSA, multilocus sequence analysis.The GenBank/EMBL/DDBJ accession numbers for the 16S rRNA gene sequences of P. eucalypti R-25678 T (5LMG 24197 T ), P. anthophila LMG 2558 T , P. deleyi R-31523 T (5LMG 24200 T ) and P. vagans R-21566 T (5LMG 24199 T ) are EF688009-EF688012, respectively, and those of strains LMG 5345, LMG 5343, LMG 2781 and R-35496 are respectively EU216734-EU216737.Detailed DNA-DNA hybridization results and a UPGMA dendrogram based on FAFLP analysis are available as supplementary material with the online version of this paper.

Pantoea theicola sp. nov., isolated from black tea

“…The phenotypic characteristics of strain QC88-366 T generally corresponded to the emended description of the genus Brady et al (2008Brady et al ( , 2009Brady et al ( , 2010Brady et al ( , 2011Brady et al ( , 2012 and Popp et al (2010). Table 1.…”

“…indologenes. Data were taken from the following sources: taxa 1-3 from this study; taxa 4-8 from Brady et al (2010); taxa 9-11 from Brady et al (2012); taxa 12-15 from Brady et al (2009); taxa 16 and 17 are type strain data from Gavini et al (1989) and Grimont & Grimont (2005); taxon 18 from Brady et al (2011); taxa 19-21 are type strains data from Mergaert et al (1993).+, 90-100 % strains positive in 1-2 days; (+), slow reaction (between 2 and 30 days); 2, negative; D, 11-89 % strains positive in 1-4 days; ND, not determined. Citrate utilization 2 + + D + + + + 2 2 + 2 2 ND 2 + + + + 2 + Indol production 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 + + 2 + Acetoin production 2 + + ND ND ND ND ND + ND ND + + + + + + + + 2 + Gelatinase 2 2 + ND ND ND ND ND ND ND ND 2 2 ND 2 + + 2 2 2 2 Acid production from:…”

Application of Whole-Cell Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry for Rapid Identification and Clustering Analysis ofPantoeaSpecies

“…Confirming the uncertainty of P. agglomerans identification, only 20 of the 53 strains received from culture collections as P. agglomerans, Pantoea spp., or E. agglomerans clustered with type strain LMG 1286 T according to gyrB sequencing. Seven strains previously assigned to P. agglomerans were found to fit in the MLSA groups of Pantoea recently described as new species (i.e., C9-1 as P. vagans; EM13cb and SC-1 as P. anthophila; LMG 5343 and ATCC 29001 as P. brenneri; and EM17cb as P. conspicua), or to belong to a novel subspecies of P. agglomerans (Eh252) (4,6,7,45). The remaining strains were reassigned to other Pantoea species or Enterobacteriaceae, although precise identification was not possible in all cases.…”

Enfermedades bacterianas de eucaliptos: Estado actual del conocimiento

“…Se especula que E. cowanii no sea un patógeno primario, sino que se comporte como endófito y que en condiciones ambientales favorables para la enfermedad contribuya con ella (Brady et al 2009a). Por último, Brady et al (2009b) identificaron tres especies nuevas, cercanamente emparentadas con P. ananatis, que provocan la misma sintomatología de tizón apical bacteriano en eucalipto: P. eucalypti Brady, P. vagans Brady y P. deleyi Brady (cuadro 2).…”