Panton-Valentine leukocidin genes and staphylococcal chromosomal cassette mec types amongst Finnish community-acquired methicillin-resistant Staphylococcus aureus strains, 1997–1999

Kardén-Lilja, M.; Ibrahem, Salha; Vuopio‐Varkila, Jaana; Salmenlinna, Saara; Lyytikäinen, Outi; Siira, Lotta; Virolainen, Anni

doi:10.1007/s10096-007-0334-0

Cited by 15 publications

(8 citation statements)

References 16 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Several studies have reported the pvl as a reliable marker for CA-MRSA strains 15 16 and a few studies 17 18 have reported the presence of the PVL toxin more in MSSA than MRSA. Our finding is in agreement with the reports from Ireland 19 and Finland 20 .…”

Section: Discussionsupporting

confidence: 94%

Molecular characterization of community- & hospital-acquired methicillin-resistant & methicillin-sensitive Staphylococcus aureus isolates in Sikkim

et al. 2015

View full text Add to dashboard Cite

Background & objectives:The two major genotypic markers that distinguish community acquired (CA) from hospital acquired (HA) methicillin resistant Staphylococcus aureus (MRSA) isolates are the architecture of mobile genetic element (SCCmec type) and presence of panton valentine leukocidin (PVL) toxin. This study was conducted to determine the molecular characteristics of CA- and HA- MRSA and methicillin sensitive S. aureus (MSSA) isolates in Sikkim.Methods:A total of 150 clinical isolates of S. aureus isolated from various clinical specimens were subjected to duplex (mec-A and pvl gene) and multiplex (SCCmec typing) PCR.Results:Of the 150 isolates, 53 (35.33%) and 66 (44%) were positive for mec-A (MRSA) and pvl genes, respectively. Thirty eight (25.33%) met the definition of CA-MRSA and 15 (10%) of HA-MRSA and the remaining 63 (42%) and 34 (22.66%) as CA- and HA-MSSA, respectively. No significant difference was seen in the distribution of PVL toxin in MRSA and MSSA isolates, but it was significantly (P<0.001) high in overall MRSA isolates than in MSSA. The majority of the MRSA isolates showed a double amplification band of SCCmec type III plus V (54.71%), and only a fewer isolates were amplified by single DNA fragments of type I (1.88%), III (3.77%), IVa (1.88%) and V (11.32%). SCCmec types I, III, IVa, were found only in HA-MRSA isolates, whereas type V in both the CA- and HA-MRSA. AST pattern showed that 18.42 per cent (7/38) and 46.66 per cent (7/15) were multidrug resistant (MDR)-CA-MRSA and MDR-HA-MRSA, respectively.Interpretation & conclusions:The present results show that SCCmec type V MRSA has been on the rise, and genotypic markers such as pvl gene detection used for the differentiation of these clinically distinct isolates of MRSA may not be reliable.

show abstract

Section: Discussionsupporting

confidence: 94%

Molecular characterization of community- & hospital-acquired methicillin-resistant & methicillin-sensitive Staphylococcus aureus isolates in Sikkim

et al. 2015

View full text Add to dashboard Cite

show abstract

“…Previous studies also have shown variations in SCCmec cassettes. These include (i) strains containing both SCCmec type IV and ccrC, (ii) strains carrying multiple ccr genes (3,7,14,26), (iii) strains carrying ccr genes without a mec complex, (iv) strains carrying a mec complex without ccr genes, and (v) a mecA-positive MRSA strain with neither ccr genes nor a mec complex (3,25,26). In our study, only mecA from three MR-CNS strains could be detected.…”

Section: Discussionmentioning

confidence: 48%

Carriage of Methicillin-Resistant Staphylococci and Their SCC mec Types in a Long-Term-Care Facility

Ibrahem¹,

Salmenlinna²,

Virolainen³

et al. 2009

J Clin Microbiol

View full text Add to dashboard Cite

Coagulase-negative staphylococci (CNS) belong to the normal microbial flora of the skin and mucous membranes of humans. The most frequently encountered CNS species in humans, in decreasing order of occurrence, are Staphylococcus epidermidis, Staphylococcus haemolyticus, Staphylococcus saprophyticus, and Staphylococcus lugdunensis (8). CNS are an important cause of nosocomial infections, particularly causing foreign device-related infections and infections among immunocompromised patients. In a recent prospective laboratorybased surveillance in four Finnish acute-care hospitals, 76% of the blood culture CNS isolates were resistant to methicillin (meticillin) (23

show abstract

“…During 1997 to 1999, only one PVL-positive strain type (FIN-11, ST80: IV, t044) was community associated (5). In the present study, a USA300 variant (FIN-25, ST8;IV, t008) which first appeared in 2004 was the third most common CA-MRSA strain type.…”

mentioning

confidence: 41%

Community-Associated Methicillin-Resistant Staphylococcus aureus Isolated in Finland in 2004 to 2006

et al. 2009

View full text Add to dashboard Cite

show abstract

Panton-Valentine leukocidin genes and staphylococcal chromosomal cassette mec types amongst Finnish community-acquired methicillin-resistant Staphylococcus aureus strains, 1997–1999

Cited by 15 publications

References 16 publications

Molecular characterization of community- & hospital-acquired methicillin-resistant & methicillin-sensitive Staphylococcus aureus isolates in Sikkim

Molecular characterization of community- & hospital-acquired methicillin-resistant & methicillin-sensitive Staphylococcus aureus isolates in Sikkim

Carriage of Methicillin-Resistant Staphylococci and Their SCC mec Types in a Long-Term-Care Facility

Community-Associated Methicillin-Resistant Staphylococcus aureus Isolated in Finland in 2004 to 2006

Contact Info

Product

Resources

About