Panton-Valentine leukocidin (PVL) gene carriage among Staphylococcus aureus strains with SCCmec types I, III, IV, and V recovered from cystic fibrosis pediatric patients in Brazil

Lima, Danielle Ferreira; Brazão, Nathalia Brito Veloso; Folescu, Tânia Wrobel; Neves, Felipe Piedade Gonçalves; Ferreira, Alex Guerra; Santos, Erica Aparecida de Oliveira; Marques, Elizabeth Andrade; Leão, Robson Souza

doi:10.1016/j.diagmicrobio.2013.10.004

Cited by 24 publications

(31 citation statements)

References 37 publications

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“…Carriage of MRSA and infection are becoming increasingly common among CF patients; the prevalence of MRSA in CF patients in the United States is approximately 25%. The molecular epidemiology and Panton-Valentine leukocidin gene carriage are complex (107)(108)(109). MRSA possess an intrinsic ability to generate highly methicillin-resistant subclones at a frequency of 10 Ϫ4 to 10 Ϫ8 , so any MRSA population represents a heterogeneously ␤-lactamresistant population (110,111), even in the absence of ␤-lactam exposure.…”

Section: Heteroresistance a Resistance Reservoir Without Selective Pmentioning

confidence: 99%

Pharmacokinetics and Pharmacodynamics of Aerosolized Antibacterial Agents in Chronically Infected Cystic Fibrosis Patients

Dalhoff¹

2014

Clin Microbiol Rev

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SUMMARY Bacteria adapt to growth in lungs of patients with cystic fibrosis (CF) by selection of heterogeneously resistant variants that are not detected by conventional susceptibility testing but are selected for rapidly during antibacterial treatment. Therefore, total bacterial counts and antibiotic susceptibilities are misleading indicators of infection and are not helpful as guides for therapy decisions or efficacy endpoints. High drug concentrations delivered by aerosol may maximize efficacy, as decreased drug susceptibilities of the pathogens are compensated for by high target site concentrations. However, reductions of the bacterial load in sputum and improvements in lung function were within the same ranges following aerosolized and conventional therapies. Furthermore, the use of conventional pharmacokinetic/pharmacodynamic (PK/PD) surrogates correlating pharmacokinetics in serum with clinical cure and presumed or proven eradication of the pathogen as a basis for PK/PD investigations in CF patients is irrelevant, as minimization of systemic exposure is one of the main objectives of aerosolized therapy; in addition, bacterial pathogens cannot be eradicated, and chronic infection cannot be cured. Consequently, conventional PK/PD surrogates are not applicable to CF patients. It is nonetheless obvious that systemic exposure of patients, with all its sequelae, is minimized and that the burden of oral treatment for CF patients suffering from chronic infections is reduced.

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Section: Heteroresistance a Resistance Reservoir Without Selective Pmentioning

confidence: 99%

Pharmacokinetics and Pharmacodynamics of Aerosolized Antibacterial Agents in Chronically Infected Cystic Fibrosis Patients

Dalhoff¹

2014

Clin Microbiol Rev

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“…Epidemic MRSA clones have been found widespread in several regions around the world. Some of these clones were also found among patients with CF, including the Southern Germany (Molina et al 2008), USA300, UK-EMRSA-3, and USA800/paediatric clones (Cocchi et al 2011, Lima et al 2014). …”

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confidence: 99%

“…Until recently, there were no reports of another clone, ST398, which belongs to clonal complex (CC) 398, in patients with CF (Lima et al 2014). This clone, known as livestock-associated methicillin-resistant S. aureus (LA-MRSA), first emerged among animals; however, it has already caused humans infections, such as folliculitis, osteomyelitis, endocarditis, and skin and soft tissue infections (van Belkum et al 2008, Grisold et al 2010, Schijffelen et al 2010).…”

mentioning

confidence: 99%

“…In the same month, after returning from her holiday, she suffered a respiratory exacerbation that was treated with intravenous antibiotics. During a previous molecular characterisation of MRSA isolates, one was identified as an ST398 clone obtained from her sputum sample (Lima et al 2014). …”

mentioning

confidence: 99%

“…Vancomycin susceptibility was determined using broth microdilution and by molecular typing of staphylococcal cassette chromosome mec (SCC mec ) and Panton-Valentine leucocidin (PVL) genes (Lima et al 2014). …”

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confidence: 99%

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Genomic information on multidrug-resistant livestock-associated methicillin-resistant Staphylococcus aureus ST398 isolated from a Brazilian patient with cystic fibrosis

Lima

Cohen

Rocha

et al. 2017

Mem. Inst. Oswaldo Cruz

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Alarmingly, the isolation of methicillin-resistant Staphylococcus aureus (MRSA) has been increasing among patients with cystic fibrosis (CF). During a previous molecular characterisation of MRSA isolates obtained from patients with CF from Rio de Janeiro, Brazil, one isolate was identified as the ST398 clone, a livestock-associated (LA) MRSA. In this study, we report the draft genome sequence of an LA-MRSA ST398 clone isolated from a patient with CF.

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Epidemiological characterization of clinical isolates of meticillin resistant Staphylococcus aureus through multilocus sequence typing and staphylococcal cassette chromosome mec typing in Northwest Iran

Sheykhsaran,

Sadeghi,

Memar

et al. 2024

Mol Biol Rep

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Panton-Valentine leukocidin (PVL) gene carriage among Staphylococcus aureus strains with SCCmec types I, III, IV, and V recovered from cystic fibrosis pediatric patients in Brazil

Cited by 24 publications

References 37 publications

Pharmacokinetics and Pharmacodynamics of Aerosolized Antibacterial Agents in Chronically Infected Cystic Fibrosis Patients

Pharmacokinetics and Pharmacodynamics of Aerosolized Antibacterial Agents in Chronically Infected Cystic Fibrosis Patients

Genomic information on multidrug-resistant livestock-associated methicillin-resistant Staphylococcus aureus ST398 isolated from a Brazilian patient with cystic fibrosis

Epidemiological characterization of clinical isolates of meticillin resistant Staphylococcus aureus through multilocus sequence typing and staphylococcal cassette chromosome mec typing in Northwest Iran

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