Papaya shoot tip associated endophytic bacteria isolated from in vitro cultures and host–endophyte interaction in vitro and in vivo

Thomas, Pious; Kumari, Sima; Swarna, Ganiga K.; Gowda, T. K. S.

doi:10.1139/w06-141

Cited by 52 publications

(51 citation statements)

References 39 publications

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“…Moreover, previous studies reported that P. ananatis was isolated as an endophytic PGPR from ginseng, papaya, and maize Rijavec et al, 2007;Thomas et al, 2007). This study shows, for the first time, the successful treatment of pepper plants with newly identified P. ananatis B1-9 leading to an increased pepper fruit yield.…”

Section: Discussionmentioning

confidence: 55%

Growth Promotion of Pepper Plants by Pantoea ananatis B1-9 and its Efficient Endophytic Colonization Capacity in Plant Tissues

Kim¹,

Cho²,

Kim³

et al. 2012

The Plant Pathology Journal

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The bacteria B1-9 that was isolated from the rhizosphere of the green onion could promote growth of pepper, cucumber, tomato, and melon plants. In particular, pepper yield after B1-9 treatment on the seedling was increased about 3 times higher than that of control plants in a field experiment. Partial 16S rDNA sequences revealed that B1-9 belongs to the genus Pantoea ananatis. Pathogenecity tests showed non-pathogenic on kimchi cabbage, carrot, and onion. The functional characterization study demonstrated B1-9's ability to function in phosphate solubilization, sulfur oxidation, nitrogen fixation, and indole-3-acetic acid production. To trace colonization patterns of B1-9 in pepper plant tissues, we used DRAQ5™ fluorescent dye, which stains the DNAs of bacteria and plant cells. A large number of B1-9 cells were found on the surfaces of roots and stems as well as in guard cells. Furthermore, several colonized B1-9 cells resided in inner cortical plant cells. Treatment of rhizosphere regions with strain B1-9 can result in efficient colonization of plants and promote plant growth from the seedling to mature plant stage. In summary, strain B1-9 can be successfully applied in the pepper plantation because of its high colonization capacity in plant tissues, as well as properties that promote efficient plant growth.

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Section: Discussionmentioning

confidence: 55%

Growth Promotion of Pepper Plants by Pantoea ananatis B1-9 and its Efficient Endophytic Colonization Capacity in Plant Tissues

Kim¹,

Cho²,

Kim³

et al. 2012

The Plant Pathology Journal

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“…The third possible origin of the nonpathogenic clones whose RAPD patterns differ from those of the inoculated strain (option iii) is that they were present as endophytes in the inoculated plants or reached the plant from external sources, which is, in our opinion, the likeliest explanation. The occurrence of endophytic agrobacteria in otherwise healthy herbaceous and woody plants has been repeatedly shown by other authors (27,49,50,53,57). Conversely, it was reported that Agrobacterium populations apparently decline rapidly in water, although they can enter a viable but nonculturable state (26,46), and it is possible that they could recover from this state and colonize plants.…”

Section: Vol 75 2009 Virulence Of a Tumefaciens Strains From Tumormentioning

confidence: 60%

Recovery of Nonpathogenic Mutant Bacteria from Tumors Caused by Several Agrobacterium tumefaciens Strains: a Frequent Event?

Llop

Murillo

Lastra

et al. 2009

Appl Environ Microbiol

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We have evaluated the interaction that bacterial genotypes and plant hosts have with the loss of pathogenicity in tumors, using seven Agrobacterium tumefaciens strains inoculated on 12 herbaceous and woody hosts. We performed a screening of the agrobacteria present inside the tumors, looking for nonpathogenic strains, and found a high variability of those strains in this niche. To verify the origin of the putative nonpathogenic mutant bacteria, we applied an efficient, reproducible, and specific randomly amplified polymorphic DNA analysis method. In contrast with previous studies, we recovered a very small percentage (0.01%) of nonpathogenic strains that can be considered true mutants. Of 5,419 agrobacterial isolates examined, 662 were nonpathogenic in tomato, although only 7 (from pepper and tomato tumors induced by two A. tumefaciens strains) could be considered to derive from the inoculated strain. Six mutants were affected in the transferred DNA (T-DNA) region; one of them contained IS426 inserted into the iaaM gene, whereas the whole T-DNA region was apparently deleted in three other mutants, and the virulence of the remaining two mutants was fully restored with the T-DNA genes as well. The plasmid profile was altered in six of the mutants, with changes in the size of the Ti plasmid or other plasmids and/or the acquisition of new plasmids. Our results also suggest that the frequent occurrence of nonpathogenic clones in the tumors is probably due to the preferential growth of nonpathogenic agrobacteria, of either endophytic or environmental origin, but different from the bacterial strain inducing the tumor.Agrobacterium tumefaciens is the causal agent of crown gall, a neoplastic disease induced in a large number of dicotyledonous and some monocotyledonous plants, as well as in gymnosperms, in this case artificially (9,32,34,35). Many studies have focused on the molecular understanding of the process that involves the tumorous growth of plant tissue by means of the transfer of specific genes from a plasmid harbored by the bacterium, called tumor-inducing plasmid (pTi) (7,18,30,43). This transfer system has significant importance for pathogenicity and epidemiology studies, as well as for biotechnology, because this unique mechanism of pathogenicity has allowed the development of a diverse and efficient methodology for the transformation of eukaryotic cells. Other studies have concentrated on the ecology and epidemiology of Agrobacterium spp. to obtain a better knowledge of the spread and stability of the bacterium as a pathogen in tumors, hosts, and soil habitats (5,11,36).The pathogenicity of A. tumefaciens seems to be somewhat unstable both in association with plants and under free-living conditions. Several studies demonstrated that A. tumefaciens populations from tumors induced under natural conditions in several plants consisted mostly of nonpathogenic strains (2, 33, 38), and loss of pathogenicity has been proposed as a likely cause to explain the predominance of nonpathogens over pathogenic strains. Lik...

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“…Although some studies have reported P. ananatis isolation from economically important crops, such as coffee (Nunes and de Melo, 2006), ginseng , papaya (Thomas et al, 2007), maize (Rijavec et al, 2007), and rice (Mano and Morisaki, 2008), they have failed to ascertain the essential growth parameters of this bacterium.…”

Section: Discussionmentioning

confidence: 99%

Isolation of Pantoea ananatis from sugarcane and characterization of its potential for plant growth promotion

et al. 2015

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Papaya shoot tip associated endophytic bacteria isolated from in vitro cultures and host–endophyte interaction in vitro and in vivo

Cited by 52 publications

References 39 publications

Growth Promotion of Pepper Plants by Pantoea ananatis B1-9 and its Efficient Endophytic Colonization Capacity in Plant Tissues

Growth Promotion of Pepper Plants by Pantoea ananatis B1-9 and its Efficient Endophytic Colonization Capacity in Plant Tissues

Recovery of Nonpathogenic Mutant Bacteria from Tumors Caused by Several Agrobacterium tumefaciens Strains: a Frequent Event?

Isolation of Pantoea ananatis from sugarcane and characterization of its potential for plant growth promotion

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