Paper-based colorimetric enzyme linked immunosorbent assay fabricated by laser induced forward transfer

Katis, Ioannis; Holloway, Judith A.; Madsen, Jens; Faust, Saul N; Garbis, Spiros D.; Smith, Peter J. S.; Voegeli, David; Bader, Dan L.; Eason, R.W.; Sones, C.L.

doi:10.1063/1.4878696

Cited by 24 publications

(12 citation statements)

References 36 publications

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“…This makes them particularly challenging, since the alteration of that structure (denaturing) completely deprives proteins of their functionality. Nevertheless, LIFT has been extensively proved feasible to print a broad range of proteins without altering their selectivity: horseradish peroxidase, bovine serum albumin, enzymes, antigens, streptavidin, biotin‐avidin, immunoglobulin G, and even the gigantic protein titin . In addition, FF‐LIFT (Section ) has also been used to print biomolecules, namely immunoglobulin G and DNA …”

Section: Applicationsmentioning

confidence: 99%

Laser‐Induced Forward Transfer: Fundamentals and Applications

Serra

Piqué

2018

Adv Materials Technologies

274

191

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Laser‐induced forward transfer (LIFT) is a digital printing technique that uses a pulsed laser beam as the driving force to project material from a donor thin film toward the receiving substrate whereon that material will be finally deposited as a voxel. This working principle allows LIFT to operate with both solid and liquid donor films, which provides the technique with an unprecedented broad spectrum of printable materials, and thus makes it very competitive over other digital technologies, like inkjet printing. It is not only that LIFT can access a much wider range of ink viscosities and loading particle sizes; the possibility of printing from solid films allows the single‐step printing of multilayers and entire devices, and even makes possible 3D printing. This versatility translates, in turn, into a broad field of applications, from graphics production to printed electronics, from the fabrication of chemical sensors to tissue engineering. This monograph provides an extensive review of the LIFT technique, from its origins to the most recent achievements, focusing on the fundamental aspects of both its working principle and transfer dynamics, as well as on its broad range of applications.

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Section: Applicationsmentioning

confidence: 99%

Laser‐Induced Forward Transfer: Fundamentals and Applications

Serra

Piqué

2018

Adv Materials Technologies

274

191

View full text Add to dashboard Cite

show abstract

“…Among the various types of LOC available, microfluidic paper-based analytical devices (lPADs), first proposed by Whitesides' group 5 in 2007, have attracted particular attention due to their low cost and ease of fabrication, which render them ideally suited to disposable POC applications. 6,7 Reviews on recent work and perspectives in the field of lPADs are given by Li et al 8 and Yetisen et al 9 The detection and analysis of proteins and DNA play a key role in disease diagnosis, and makes possible the delivery of preventative treatment if conducted sufficiently early. [10][11][12][13][14] However, the target proteins and DNA are generally diluted in sample solutions during the early stage of a disease, and thus collecting a sufficient number of molecules or concentration for analysis purposes is problematic.…”

Section: Introductionmentioning

confidence: 99%

Ion concentration polarization on paper-based microfluidic devices and its application to preconcentrate dilute sample solutions

Yang

Wang

2015

Biomicrofluidics

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Microfluidic paper-based analytical devices (μPADs) are a promising solution for a wide range of point-of-care applications. The feasibility of inducing ion concentration polarization (ICP) on μPADs has thus far attracted little attention. Accordingly, this study commences by demonstrating the ICP phenomenon in a μPAD with a Nafion ion-selective membrane. We are the first to measure the current-voltage curve on a Nafion-coated μPAD in order to indicate that the ion depletion occurs and the ICP is triggered when the current reaches the limiting current. The ICP effect is then exploited to preconcentrate fluorescein on μPADs incorporating straight and convergent channels. By an optimal geometric design, it is shown that the convergent channel results in a greater preconcentration effect than the straight channel. Specifically, a 20-fold enhancement in the sample concentration is achieved after 130 s given an initial concentration of 10−5 M and an external potential of 50 V. By contrast, the straight channel yields only a 10-fold improvement in the concentration after 180 s. Further, the practical feasibility of the proposed convergent-channel μPAD is demonstrated using fluorescein isothiocyanate labeled bovine serum albumin. The experimental results show that a 15-fold enhancement of the initial sample concentration (10−5 M) is obtained after 120 s given an external potential of 50 V.

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“…[1][2][3][4] lPADs differ from traditional Lab-on-a-Chip (LoC) technologies in that the fluid is transported by capillary forces rather than a pump. Moreover, lPADs can be fabricated with either hydrophobic or hydrophilic channels using a simple wax printer.…”

Section: Introductionmentioning

confidence: 99%

Colored wax-printed timers for two-dimensional and three-dimensional assays on paper-based devices

et al. 2014

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Microfluidic paper-based analytical devices (lPADs) are widely used for performing diagnostic assays. However, in many assays, time-delay valves are required to improve the sensitivity and specificity of the results. Accordingly, this study presents a simple, low-cost method for realizing time-delay valves using a color wax printing process. In the proposed approach, the time-delay effect is controlled through a careful selection of both the color and the saturation of the wax content. The validity of the proposed method is demonstrated by performing nitrite and oxalate assays using both a simple two-dimensional lPAD and a three-dimensional lPAD incorporating a colored wax-printed timer. The experimental results confirm that the flow time can be controlled through an appropriate selection of the color and the wax content. In addition, it is shown that nitrite and oxalate assays can be performed simultaneously on a single device. In general, the results presented in this study show that the proposed lPADs provide a feasible low-cost alternative to conventional methods for performing diagnostic assays. V C 2014 AIP Publishing LLC.

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Paper-based colorimetric enzyme linked immunosorbent assay fabricated by laser induced forward transfer

Cited by 24 publications

References 36 publications

Laser‐Induced Forward Transfer: Fundamentals and Applications

Laser‐Induced Forward Transfer: Fundamentals and Applications

Ion concentration polarization on paper-based microfluidic devices and its application to preconcentrate dilute sample solutions

Colored wax-printed timers for two-dimensional and three-dimensional assays on paper-based devices

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