Paper-based colorimetric sensor for easy and simple detection of polygalacturonase activity aiming for diagnosis of Allium white rot disease

Choi, Young-Soo; Lee, Mi Rha; Yang, Kwang-Yeol; Kim, Cheol Soo; Lee, Kyeong–Hwan

doi:10.1016/j.aca.2020.04.008

Cited by 8 publications

(1 citation statement)

References 23 publications

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“…The sample is put onto a test strip, and the result is displayed within 5 to 10 minutes, using the paper-based lateral flow immunochromatographic analysis test, which analyzes the quality and quantity of the material in a complicated mixture [75]. A paper-based colorimetric sensor was used to predict plant diseases in Allium crops for the easy and simple measurement of PG activity with the help of the principle of the ruthenium red (RR) dye method [149]; however, the drawback of hindrance can be developed by blurry signaling at the detection point due to capillary reaction forces on the liquid and its corresponding membrane [150]. Figure 4 illustrates the fundamental functioning of a microfluid paper-based biosensor, implemented with the colorimetric methodology for the observation of color change in case of any targeted toxins detection.…”

Section: Paper-based Sensingmentioning

confidence: 99%

Scope of Onsite, Portable Prevention Diagnostic Strategies for Alternaria Infections in Medicinal Plants

et al. 2023

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Medicinal plants are constantly challenged by different biotic inconveniences, which not only cause yield and economic losses but also affect the quality of products derived from them. Among them, Alternaria pathogens are one of the harmful fungal pathogens in medicinal plants across the globe. Therefore, a fast and accurate detection method in the early stage is needed to avoid significant economic losses. Although traditional methods are available to detect Alternaria, they are more time-consuming and costly and need good expertise. Nevertheless, numerous biochemical- and molecular-based techniques are available for the detection of plant diseases, but their efficacy is constrained by differences in their accuracy, specificity, sensitivity, dependability, and speed in addition to being unsuitable for direct on-field studies. Considering the effect of Alternaria on medicinal plants, the development of novel and early detection measures is required to detect causal Alternaria species accurately, sensitively, and rapidly that can be further applied in fields to speed up the advancement process in detection strategies. In this regard, nanotechnology can be employed to develop portable biosensors suitable for early and correct pathogenic disease detection on the field. It also provides an efficient future scope to convert innovative nanoparticle-derived fabricated biomolecules and biosensor approaches in the diagnostics of disease-causing pathogens in important medicinal plants. In this review, we summarize the traditional methods, including immunological and molecular methods, utilized in plant-disease diagnostics. We also brief advanced automobile and efficient sensing technologies for diagnostics. Here we are proposing an idea with a focus on the development of electrochemical and/or colorimetric properties–based nano-biosensors that could be useful in the early detection of Alternaria and other plant pathogens in important medicinal plants. In addition, we discuss challenges faced during the fabrication of biosensors and new capabilities of the technology that provide information regarding disease management strategies.

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Section: Paper-based Sensingmentioning

confidence: 99%