Paper-based diagnostic devices for clinical paraquat poisoning diagnosis

Kuan, Chen-Meng; Lin, Szu‐Ting; Yen, Tzung‐Hai; Wang, Yulin; Cheng, Chao‐Min

doi:10.1063/1.4953257

Cited by 19 publications

(23 citation statements)

References 50 publications

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“…For this experiment, we designed a simple microfluidic pattern using Microsoft PowerPoint software (Microsoft, Redmond, WA, USA)3637. The full length of the pattern is 2.8 mm; the circular diameter is 0.4 mm.…”

Section: Methodsmentioning

confidence: 99%

“…We printed the pattern on chromatography paper (Grade 1 Qualitative Filter Papers, GE Healthcare Life Sciences, Little Chalfont, Bucks, UK), as shown in Fig. 1 using wax-printer (Phaser 8560 wax printer, Xerox, Norwalk, CT, USA)3637. After baking the paper using the patterned wax at 105 °C for 3 min, hydrophilic-hydrophobic regions formed inside the chromatography paper.…”

Section: Methodsmentioning

confidence: 99%

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Relationship between Porcine Sperm Motility and Sperm Enzymatic Activity using Paper-based Devices

Matsuura

Huang

Chen

et al. 2017

Sci Rep

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Mammalian sperm motility has traditionally been analyzed to determine fertility using computer-assisted semen analysis (CASA) systems. To develop low-cost and robust male fertility diagnostics, we created a paper-based MTT assay and used it to estimate motile sperm concentration. When porcine sperm motility was inhibited using sperm enzyme inhibitors for sperm enzymes related to mitochondrial activity and glycolysis, we simultaneously recorded sperm motility and enzymatic reactivity using a portable motility analysis system (iSperm) and a paper-based MTT assay, respectively. When using our paper-based MTT-assay, we calculated the area mean value signal intensity (AMV) to evaluate enzymatic reactivity. Both sperm motility and AMV decreased following treatment with iodoacetamide (IODO) and 3-bromopyruvic acid (3BP), both of which are inhibitors of glycolytic enzymes including glyceraldehyde-3-phosphate dehydrogenase (GAPDH). We found a correlation between recorded motility using iSperm and AMV from our paper-based assay (P < 0.05), suggesting that a sperm-related enzymatic reaction is involved in sperm motility. Under this protocol, MTT reduction was coupled with catalysis of GAPDH and was promoted by electron transfer from NADH. Based on this inhibitor study, sperm motility can be estimated using our paper-based MTT-assay.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Relationship between Porcine Sperm Motility and Sperm Enzymatic Activity using Paper-based Devices

Matsuura

Huang

Chen

et al. 2017

Sci Rep

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“…The HEMT biosensor can be used in personal health care products as it is inexpensive, easy to integrate into a portable system, and can be used by anyone without prior training or knowledge. (29) In the future, this technology can pave the way for rapid diagnostics of a panel of CVD biomarkers for better disease risk assessment and prevention.…”

Section: Discussionmentioning

confidence: 99%

Aptamer-functionalized AlGaN/GaN High-electron-mobility Transistor for Rapid Diagnosis of Fibrinogen in Human Plasma

Tai

Sinha

Sarangadharan

et al. 2018

Sensors and Materials

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In this research, we have developed a rapid diagnostic assay using a high-electron-mobility transistor (HEMT)-based biosensor to screen for fibrinogen in clinical plasma samples. High sensitivity over a wide detection range (0-5 g/L), good selectivity, low-cost fabrication, portability, and simplicity of use are key features of our biosensor. Early diagnosis of heart disease in low-, medium-, and high-risk patients is possible with an assay turnaround time of 5 min and a plasma sample volume of 5-10 µL. High sensitivity and low detection limits (0.5 g/L) are made possible in environments with high salt concentrations because the HEMT sensor design overcomes the charge screening effect associated with a very small Debye length. This biosensor technology demonstrates the potential for development as a point-of-care or homecare diagnostic device for quick and easy assessment of cardiovascular disease (CVD) risk.

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“…We then loaded 8 µL of sample onto the test zone and allowed it to rest for 10 min at 25 • C. Detection results were recorded using a digital camera and the signal was analyzed using ImageJ software. The RGB color value of each detection result was obtained, and the R value was used for further calculation [13,36]. We subtracted original test zone background values for each detection result.…”

Section: Performing Origami-based Detection Of Paraquatmentioning

confidence: 99%

“…However, successful detection of multiple analytes using a single PAD relied on maintaining particular, sometimes critical, conditions such as moisture level. In a previous study, we showed that paraquat detection in a PAD required a wet environment [13,36]. Traditional ELISA, however, requires application of multiple reagents, and the time to complete such a process using paper increases the likelihood of any moisture-sensitive components drying out.…”

Section: O-pad For Paraquat Detectionmentioning

confidence: 99%

Two Potential Clinical Applications of Origami-Based Paper Devices

et al. 2019

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Detecting small amounts of analyte in clinical practice is challenging because of deficiencies in specimen sample availability and unsuitable sampling environments that prevent reliable sampling. Paper-based analytical devices (PADs) have successfully been used to detect ultralow amounts of analyte, and origami-based PADs (O-PADs) offer advantages that may boost the overall potential of PADs in general. In this study, we investigated two potential clinical applications for O-PADs. The first O-PAD we investigated was an origami-based enzyme-linked immunosorbent assay (ELISA) system designed to detect different concentrations of rabbit IgG. This device was designed with four wing structures, each of which acted as a reagent loading zone for pre-loading ELISA reagents, and a central test sample loading zone. Because this device has a low limit of detection (LOD), it may be suitable for detecting IgG levels in tears from patients with a suspected viral infection (such as herpes simplex virus (HSV)). The second O-PAD we investigated was designed to detect paraquat levels to determine potential poisoning. To use this device, we sequentially folded each of two separate reagent zones, one preloaded with NaOH and one preloaded with ascorbic acid (AA), over the central test zone, and added 8 µL of sample that then flowed through each reagent zone and onto the central test zone. The device was then unfolded to read the results on the test zone. The three folded layers of paper provided a moist environment not achievable with conventional paper-based ELISA. Both O-PADs were convenient to use because reagents were preloaded, and results could be observed and analyzed with image analysis software. O-PADs expand the testing capacity of simpler PADs while leveraging their characteristic advantages of convenience, cost, and ease of use, particularly for point-of-care diagnosis.

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Paper-based diagnostic devices for clinical paraquat poisoning diagnosis

Cited by 19 publications

References 50 publications

Relationship between Porcine Sperm Motility and Sperm Enzymatic Activity using Paper-based Devices

Relationship between Porcine Sperm Motility and Sperm Enzymatic Activity using Paper-based Devices

Aptamer-functionalized AlGaN/GaN High-electron-mobility Transistor for Rapid Diagnosis of Fibrinogen in Human Plasma

Two Potential Clinical Applications of Origami-Based Paper Devices

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