Paper-based microfluidic devices based on 3D network polymer hydrogel for the determination of glucose in human whole blood

He, Rong-Yu; Tseng, Hsin-Yi; Lee, Hsia-An; Liu, Yu-Ci; Koshevoy, Igor O.; Pan, Shiann; Ho, Mei‐Lin

doi:10.1039/c9ra04278d

Cited by 15 publications

(6 citation statements)

References 38 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…He et al also found that 2 μl of blood is the best blood volume. [77] Using this method, analysis of the sample can be completed in 1 min, and the detection results are consistent with the results obtained using the blood glucometer, proving the feasibility of the analytical device. To prove the validity of this method, a sample with a blood glucose concentration of 3-200 mM was used to form a comparison with a human whole blood sample in the experiment.…”

Section: Microfluidic Applications In Blood Testingsupporting

confidence: 79%

“…(D) Sample experiments of whole blood on μPAD based on different papers. [77] (Color figure can be viewed at the Royal Society of Chemistry) plasma from red blood cells agglutination. Recently, Tseng et al [84] developed a 3D μPAD to detect the human whole blood creatinine.…”

Section: Microfluidic Applications In Blood Testingmentioning

confidence: 99%

“…The equipment was realized by heating an alkaline Jaffe reaction and then performing colorimetric detection. He et al [77] proposed an optical microfluidic paper analysis device on a single sheet of paper for detecting glucose from a small sample size (2 μl) of whole blood samples. The device is a mushroom-shaped analytical device, which includes a sample inlet area and detection area, as shown in Figure 3C.…”

Section: Microfluidic Applications In Blood Testingmentioning

confidence: 99%

See 2 more Smart Citations

Polymer‐based microfluidic devices: A comprehensive review on preparation and applications

Han

Zhang

Tian

et al. 2021

Polymer Engineering & Sci

View full text Add to dashboard Cite

As key components in life science, microfluidics devices play increasingly important roles in the fields of biomedicine, pharmaceuticals, and biology due to its high detection efficiency. Also, microfluidics devices have many applications in label-free detection, thanks to their low cost and ease of operation.Traditionally, microfluidic devices are fabricated with glass and silicon materials. As an alternative, polymer materials are commonly used in the fabrication of microfluidic devices for their lower cost, versatile fabrication methods, biocompatibility, and repeatability. In this review, the methods for fabricating polymer microfluidic systems are described in detail, including lithography, hot embossing and imprinting, soft lithography, laser ablation, 3D printing, and other technologies. In particular, the merits and demerits of each of the methods are discussed comprehensively. This review also focuses on the applications of polymer-based microfluidic systems in the areas of biology, medicine and production, typical application scenarios including blood, tumor cell and nucleic acid testing, oil adsorption detection, and pesticide detection. This review emphasizes the device preparation methods and application scenarios of polymer-based microfluidic systems and was expected to arise more extensive discussions in this field.

show abstract

Section: Microfluidic Applications In Blood Testingsupporting

confidence: 79%

Section: Microfluidic Applications In Blood Testingmentioning

confidence: 99%

Section: Microfluidic Applications In Blood Testingmentioning

confidence: 99%

See 1 more Smart Citation

Polymer‐based microfluidic devices: A comprehensive review on preparation and applications

Han

Zhang

Tian

et al. 2021

Polymer Engineering & Sci

View full text Add to dashboard Cite

show abstract

“…Immobilization of glucose sensing reagents in the gel material is important for ensuring the operational stability of glucose biosensor response . A number of papers have reported the immobilization of glucose-sensing reagents in gel to perform glucose sensing and applied such methods to various sensing mechanisms, including electrochemical, fluorescence, , surface plasmon resonance, , luminescence, , and colorimetry , methods. Colorimetry is one of the simplest forms of detection and thus commonly used in many glucose POC diagnosis methods.…”

Section: Resultsmentioning

confidence: 99%

A Gel-Based Separation-Free Point-of-Care Device for Whole Blood Glucose Detection

et al. 2020

View full text Add to dashboard Cite

This article introduces a gel-based separation-free point-ofcare (POC) device for whole blood glucose colorimetric detection. Enzymes and a chromogenic substrate needed for colorimetric detection of glucose were entrapped in a photopolymerized poly(ethylene) glycol diacrylate (PEG-DA) hydrogel that was cast-molded into a circular shape. Our method enables colorimetric detection without the need for preseparation of blood plasma as the nanometer-scale three-dimensional porous structure of the hydrogel allows the diffusion of small analytes such as glucose while blocking the much larger blood cells. Our method requires less enzymatic concentration and, hence, offers a cost-saving benefit. In addition, PEG-DA also acts as an enzyme stabilizer, and the shelf-life testing result shows that enzyme activity can be maintained in PEG-DA over a long period of time. The concept of this simple, cost-effective method was demonstrated by the colorimetric detection of blood glucose directly from human whole bloodthout any sample preparation steps. The results were compared with those of a spectrophotometry method and showed relative error ranging from 5 to 19%, and less than 9% when compared with a commercial glucose meter. The presented method has the potential to be broadly utilized for other whole blood biomolecule analyses in POC testing applications.

show abstract

“…In addition to the devices for glucose detection in skin ISF including fluorescence [122], frequency [77,83], electrical [124] etc. and tear including structure color [74,87,[125][126][127], etc., there are many studies suitable for blood including structure color [115,126], fluorescence [39,40,104], electrical [109], etc. and urine including structure color [128,129], electrical [93] etc.…”

Section: Othersmentioning

confidence: 99%

Recent Applications of Point-of-Care Devices for Glucose Detection on the Basis of Stimuli-Responsive Volume Phase Transition of Hydrogel

Gao

You

2021

BioChip J

View full text Add to dashboard Cite

Diabetes is a serious global disease that threatens more than 400 million people's health. Therefore, timely detection of body's glucose level becomes extremely important for control, diagnosis and treatment of diabetes. Based on the feature of stimuli-responsive volume phase transition of hydrogel materials, this review will provide a systematic summary of glucose detection devices in recent years, including hydrogel preparation methods based on glucose-sensitive pattern, detection mechanisms based on signal transduction, current and emerging devices based on different body fluids and discuss the challenge, prospect the future development trend in the end.

show abstract

Paper-based microfluidic devices based on 3D network polymer hydrogel for the determination of glucose in human whole blood

Abstract: In this study, optical microfluidic paper analytical devices (μPADs) for glucose detection from whole blood samples with a small sample volume (2 μL) have been developed on a single paper.

Cited by 15 publications

References 38 publications

Polymer‐based microfluidic devices: A comprehensive review on preparation and applications

Polymer‐based microfluidic devices: A comprehensive review on preparation and applications

A Gel-Based Separation-Free Point-of-Care Device for Whole Blood Glucose Detection

Recent Applications of Point-of-Care Devices for Glucose Detection on the Basis of Stimuli-Responsive Volume Phase Transition of Hydrogel

Contact Info

Product

Resources

About