Paper-based microfluidic system for tear electrolyte analysis

Yetisen, Ali K.; Jiang, Nan; Tamayol, Ali; Ruiz–Esparza, Guillermo U.; Zhang, Yu Shrike; Medina-Pando, Sofía; Gupta, Aditi; Wolffsohn, James S.; Butt, Haider; Khademhosseini, Ali; Yun, Seok Hyun

doi:10.1039/c6lc01450j

Cited by 123 publications

(110 citation statements)

References 38 publications

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“…The fluorescence intensity increased by 7.4‐fold with increasing Ca 2+ ion concentration. The dissociation constant of the BAPTA for Ca 2+ ions was calculated to be 302 µmol L −1 (Equation ), which was comparable with the previously reported value (320 µmol L −1 ) …”

supporting

confidence: 90%

“…A potential approach to dry eye severity assessment and subtype differentiation is through the measurements of tear electrolytes, in which the composition differs based on the dry eye subtype. Sensing tear electrolytes can provide quantitative data to diagnose and track the course of dry eye syndrome, manage the eye drop medication dosage, as well as create a diagnostic basis for personalized eye drops …”

mentioning

confidence: 99%

“…The pH value of tear fluid increases up to 7.9 in patients with dry eye syndrome as compared to pH 7.4 in healthy individuals (Tables S1 and S2, Supporting Information) . Thus, determining the pH value of tear fluid has clinical utility in the diagnosis of dry eye syndrome.…”

mentioning

confidence: 99%

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Scleral Lens Sensor for Ocular Electrolyte Analysis

et al. 2019

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The quantitative analysis of tear analytes in point‐of‐care settings can enable early diagnosis of ocular diseases. Here, a fluorescent scleral lens sensor is developed to quantitatively measure physiological levels of pH, Na+, K+, Ca2+, Mg2+, and Zn2+ ions. Benzenedicarboxylic acid, a pH probe, displays a sensitivity of 0.12 pH units within pH 7.0–8.0. Crown ether derivatives exhibit selectivity to Na+ and K+ ions within detection ranges of 0–100 and 0–50 mmol L−1, and selectivities of 15.6 and 8.1 mmol L−1, respectively. A 1,2 bis(o‐aminophenoxy)ethane‐N,N,‐N',N'‐tetraacetic‐acid‐based probe allows Ca2+ ion sensing with 0.02–0.05 mmol L−1 sensitivity within 0.50–1.25 mmol L−1 detection range. 5‐Oxazolecarboxylic acid senses Mg2+ ions, exhibiting a sensitivity of 0.10–0.44 mmol L−1 within the range of 0.5–0.8 mmol L−1. The N‐(2‐methoxyphenyl)iminodiacetate Zn2+ ion sensor has a sensitivity of 1 µmol L−1 within the range of 10–20 µmol L−1. The fluorescent sensors are subsequently multiplexed in the concavities of an engraved scleral lens. A handheld ophthalmic readout device comprising light‐emitting diodes (LEDs) and bandpass filters is fabricated to excite as well as read the scleral sensor. A smartphone camera application and an user interface are developed to deliver quantitative measurements with data deconvolution. The ophthalmic system enables the assessment of dry eye severity stages and the differentiation of its subtypes.

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Scleral Lens Sensor for Ocular Electrolyte Analysis

et al. 2019

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“…[33] The stains on the chip are the preloaded indicators.When a4.8 mL droplet containing metal ions over the concentration of the ppm scale was dripped on the chip,i tw as divided into five microdroplets and reacted with the indicators in the sensing zones.T he specific colors in these sensing zones indicate the existence of Ni 2+ ,C u 2+ ,o rC r 6+ ions and the acidity of the sample.T his strategy can be extended to the multianalyte detection of biosamples,s uch as tears,b lood, and urine. [34,35]…”

Section: Resultsmentioning

confidence: 99%

Rücktitelbild: Droplet Precise Self‐Splitting on Patterned Adhesive Surfaces for Simultaneous Multidetection (Angew. Chem. 26/2020)

Fang

Zhao

et al. 2020

Angewandte Chemie

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Die präzise Trennung und Lokalisierung von Mikrotröpfchen sind grundlegende Arbeitsgänge in verschiedenen Forschungsbereichen, z. B. beim biologischen Screening, der kombinatorischen Analyse und bei der Erkennung komplexer Proben. In ihrem Forschungsartikel auf S. 10622 demonstrieren M. Li, Q. Li, Y. Song et al. eine Strategie zur Selbstaufspaltung von Tröpfchen, um unabhängige Mikrotröpfchen mit wohldefinierter Morphologie und Anordnung zu generieren und simultane Nachweise komplexer Analyte in einzelnen Probetröpfchen zu realisieren.

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“…Las desviaciones anormales de pH, además de predisponer a estas patologías, podrían afectar la respuesta terapéutica a antibióticos y la intolerancia a lentes de contacto. Por tanto, el monitoreo del pH en la práctica clínica podría ser de gran utilidad (Coles & Jaros, 1984;Pinna, Usai, Sechi, Carta, & Zanetti, 2011;Yetisen, Jiang, Tamayol, Ruiz-Esparza, & Zhang, 2017 Continuación de la tabla 5 la lágrima podría favorecer una adaptación más suave al lente, dado que la aceptación de este es más confortable en aquellos casos en que el pH es más alcalino (cercano a 8.0) (Motolko & Breslin, 1981).…”

Section: Discussionunclassified

Determinación de pH por colorimetría en muestras pequeñas de lágrima. Método simple para medición en enfermedades oftalmológicas de la superficie ocular anterior

Barba-Gallardo

Sandoval-Ortega

Parra

et al. 2019

IyCUAA

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No existen métodos sencillos en la práctica clínica para cuantificar el pH de lágrima. El objetivo fue estandarizar un método para la determinacióndel pH en pequeñas muestras de lágrima de pacientes. Se construyó una escala colorimétrica en un rango de pH de 6 a 8.0 utilizando como indicador azul de bromotimol, con KH2PO4 0.1M (constante) y volúmenes ascendentes de NaOH 0.1M. El método se validó en 20 uL de lágrima de73 sujetos, en quienes permitió distinguir cambios en el pH de 0.2 en 0.2 en un rango de 6.0 a 8.0 con un coeficiente de variación intrasujeto de 0.8 a 4.0%. El método aquí presentado, es preciso, con baja variación interensayo. Esto, aunado a su simpleza, bajo costo y poco volumen de muestra necesario, lo convierten en una alterativa para el monitoreo del pH de la lágrima, ya sea en la cama del paciente o el consultorio.

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Paper-based microfluidic system for tear electrolyte analysis

Abstract: This article describes a paper-based microfluidic system that quantifies the concentrations of tear electrolytes using a smartphone-based reader.

Cited by 123 publications

References 38 publications

Scleral Lens Sensor for Ocular Electrolyte Analysis

Scleral Lens Sensor for Ocular Electrolyte Analysis

Rücktitelbild: Droplet Precise Self‐Splitting on Patterned Adhesive Surfaces for Simultaneous Multidetection (Angew. Chem. 26/2020)

Determinación de pH por colorimetría en muestras pequeñas de lágrima. Método simple para medición en enfermedades oftalmológicas de la superficie ocular anterior

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