Paper chromatographic separation of multi-component antibiotic mixtures

Lees, T.M.; DeMuria, P.J.; Boegemann, W.H.

doi:10.1016/s0021-9673(01)92830-4

Cited by 11 publications

(5 citation statements)

References 3 publications

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“…In experiments with penicillin amides and methyl esters, the amount of substrate in the test system was reduced to 5 mg. Paper chromatography. The procedure employed in running paper chromatograms and bioautographs on Bacillus subtilis plates is described in detail by Lees, DeMuria, and Boegemann (1961). 6-APA and related compounds (i.e., 6-APA amide, 6-APA methyl ester, and 7-ACA) on fully developed chromatograms were made bioactive by phenylacetylation (Batchelor et al, 1959).…”

Section: 25mentioning

confidence: 99%

Distribution and Substrate Specificity of Benzylpenicillin Acylase1

Huang¹,

Seto²,

Shull³

1963

Applied Microbiology

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substrate specficity of benzylpenicillin acylase. Appl. Microbiol. 11:1-6. 1963.-Benzylpenicillin acylase, which hydrolyzes benzylpenicillin to 6-aminopenicillanic acid, was found to be widely distributed among members of the Schizoinycetes, particularly in gram-negative bacteria, and in the genus Nocardia. The hydrolysis of a series of biosynthetic and semisynthetic penicillins by freeze-dried cells of a strain of Nocardia and of Proteus was studied. Benzylpenicillin was the preferred substrate; all departures from the benzylpenicillin side-chain structure led to reduction of substrate activity (the greater the departure, the greater the reduction in activity). Penicillin amides and methyl esters were also hydrolyzed, as were suitable N-acyl derivatives of 7-aminocephalosporanic acid. Occurrence of an enzyme activity which hydrolyzes benzylpenicillinamide to benzylpenicillin was detected in certain strains of yeasts.

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Section: 25mentioning

confidence: 99%

Distribution and Substrate Specificity of Benzylpenicillin Acylase1

Huang¹,

Seto²,

Shull³

1963

Applied Microbiology

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“…Erythromycin occurs as white or slightly yellow, odorless or almost odorless, slightly hygroscopic, crystals, or powder. Unlike azithromycin, erythromycin has a higher melting point, 135-140 • C. It is optically active with [α] 25 D of −78 • at concentration of 0.0199 mg mL −1 in methanol. It exhibits characteristic UV absorption maxima when dissolved in water (pH 6.3) at 280 nm (ε = 50).…”

Section: Physicochemical Properties Of Macrolidesmentioning

confidence: 99%

“…Azithromycin has a melting point of 113-115 • C. It is levorotatory with [α] 25 D of −37 • at concentration of 0.01 mg mL −1 in chloroform. It has no characteristic UV absorption maximum in any solvent.…”

Section: Physicochemical Properties Of Macrolidesmentioning

confidence: 99%

“…Clarithromycin exhibits a higher melting point, 217-220 • C, and it is levorotatory with [α] 25 D of −90.4 • at concentration of 1 mg/mL in chloroform. Its UV-spectrum in chloroform exhibits absorbance maxima at 288 nm (ε = 27.9) and the solubility profile is like azithromycin [17].…”

Section: Physicochemical Properties Of Macrolidesmentioning

confidence: 99%

“…Lees et al [25] described the use of a paper chromatographic analysis for the separation of the acetylated oleandomycins in multicomponent antibiotic mixtures. Highpolar solvent systems were found to be suitable for the separation of oleandomycin base and its acetylated derivatives, including triacetyl-oleandomycin.…”

Section: Analytical Techniques For the Determinationnewline Of Macrolmentioning

confidence: 99%

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Application of Different Analytical Techniques and Microbiological Assays for the Analysis of Macrolide Antibiotics from Pharmaceutical Dosage Forms and Biological Matrices

Bekele

Gebeyehu

2012

ISRN Analytical Chemistry

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Macrolides are a group of drugs whose activity stems from the presence of a macrolide ring, a large macrocyclic lactone ring to which one or more deoxy sugars may be attached. They are produced by Streptomyces species and used primarily against gram-positive bacteria. The determination of antibiotics, including macrolides, is mainly carried out by microbiological assays. However, microbiological assays tended to lack specificity. And hence to overcome this problem, lots of chemical and instrumental methods have been developed to determine macrolides separately as well as simultaneously. Different chromatographic, spectrophotometric, and electrochemical methods used for the determination of macrolides have been reviewed in this paper.

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Paper chromatography of antibiotics

Betina

1965

Chromatographic Reviews

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Paper chromatographic separation of multi-component antibiotic mixtures

Cited by 11 publications

References 3 publications

Distribution and Substrate Specificity of Benzylpenicillin Acylase1

Distribution and Substrate Specificity of Benzylpenicillin Acylase1

Application of Different Analytical Techniques and Microbiological Assays for the Analysis of Macrolide Antibiotics from Pharmaceutical Dosage Forms and Biological Matrices

Paper chromatography of antibiotics

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