Paper Chromatographic Techniques for the Determination of Cephalothin and Desacetylcephalothin in Body Fluids

Abstract

Paper chromatographic procedures may be used to detect cephalothin and its metabolite desacetylcephalothin in urine, plasma, synovial fluid, and cerebrospinal fluid. Protein-bound antibiotics are released from plasma, cerebrospinal fluid, and synovial fluid by dilution with an equal volume of dimethylformamide. Data are presented on the sample preparation, paper chromatographic system, and other specific techniques.

Chromatography

“…Chromatographic Systems. Many different types of layers have been employed in addition to the usual homemade 250-µ silica gel layers: charcoal with polyethylene binder (1118); magnesium oxide for lipids (693), wax esters (1007), sterols (688), and plant constituents (687); commercial precoated cellulose and silica gel sheets for aflatoxins (1149) and trace metals (926); silicic acid mixed with glass fibers and cationic starch (872); porous glass for organic sulfur compounds (1590) and for steroids, drugs, and alkaloids (887); 5-µ silica gel layers on cellophane sheets (886); microcrystalline cellulose for metal-EDTA complexes (896); silica gel with added kaolin and montmorillonite for clay minerals (690); magnesium hydroxide for azaaromatic compounds (700); pH-gradient layers for phenothiazines (769); ZnS for metal complexes (776); Synachrom porous organic polymer beads developed with solvents of varying pH for quinolines and phenols ( 633); prefabricated roll preparations of silica gel and cellulose for steroids, amino acids, and dyes (582), polyamide for flavonoids (1462), estrogens (597), and amino acid dervatives (1154); silica gel fiber glass sheets for serum barbiturates (618) and steroids (1247); superfine Sephadex G-25 to G-200 for dansylated proteins (44I); calcium carbonate with gypsum binder for morphine and morphinan derivatives (350); cellulose layers on aluminum foil for amino acids (373); poly (vinyl acetate) for 2,4-dinitrophenyIhydrazones (328); silica gel impregnated with basic lead acetate for sugars, anthocyanins, and anthocyanidins (1081); cellulose mixed with cortical cells from wool for tannin extracts (1188); silica gel containing 5% starch and 5% NazCOs for inorganic anions (696); silanized silica gel for phydroxybenzoic acid and its esters (1140); 4:1 microcrystalline nylon Aviamide -6 -polytetrafluoroethylene Fluoroglide 200 mixed layer on Mylar for hydrocarbons (1128); silica gel containing 2% disodium EDTA for xanthones (43); silica gel impregnated with 15% thallium nitrate for monoterpene hydrocarbons (56); silica gel containing silver nitrate for Cannabis constituents (494), pyridine homologs (1389), triglycerides (151,180), and amines and carboxylic acids (1388); keratin layers for inorganic ions (155); esterified keratin for DNP-amino acids and heterocyclic bases…”

“…Gentamycin sulfate has been studied by thin-layer chromatography and the antibiotic-protein complex by zonal gel filtration on Sephadex (1471)• Amphoteric and acidic polyene antibiotics have been tested on silica gel thin-layer plates (1025). Cephalothin and its metabolite deacetylcephalothin in urine and biological fluids have been studied by paper chromatography (582). The production of cyathin has been monitored by thin-layer chromatography on silica gel G (648).…”