Paper Chromatography of Glutathione and Its Hydrolysis Products

Gutcho, Marcia; Laufer, Louis

doi:10.1016/b978-1-4832-2900-3.50011-0

Search citation statements

Order By: Relevance

Paper Sections

Select...

Methods3

A Thiol Peptide Fromn Rat Livers1

Citation Types

Supporting

Mentioning

Contrasting

Year Published

1959

2020

Publication Types

Select...

Article7

Book1

Relationship

Self Cite0

Independent8

Authors

Journals

Cited by 10 publications

(10 citation statements)

References 4 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Descending chromatography of the N-ethylmaleimide derivatives of sulfhydryl compounds (Gutcho and Laufer, 1954) was performed on Whatman no. 1 paper, using tertiary butanolformic acid-water (70:15:15) as the solvent.…”

Section: Methodsmentioning

confidence: 99%

DECOMPOSITION OF NATIVE KERATIN BY STREPTOMYCES FRADIAE

1959

View full text Add to dashboard Cite

Section: Methodsmentioning

confidence: 99%

DECOMPOSITION OF NATIVE KERATIN BY STREPTOMYCES FRADIAE

1959

View full text Add to dashboard Cite

“…These were quantitated by treating extracts (14) (15) in butanol-concentrated formic acid-H20 (7:2:1, voL/vol/vol), localization of markers with ninhydrin, fractionation, and scintillation counting. JTG10 had <0.4% of the wild-type GSH level, compared to 1.4% residual GSH in strain BS53.…”

mentioning

confidence: 99%

Glutathione in Escherichia coli is dispensable for resistance to H2O2 and gamma radiation

Greenberg¹,

Demple²

1986

J Bacteriol

141

View full text Add to dashboard Cite

show abstract

“…Cysteinylglycine (CG) and cystinyl-bis-glycine (CbG) were synthesized by a modification of the method reported by Gutcho and Laufer (CAS Registry, CG [19246–18–5]; CbG [7729–20–6]) …”

Section: Methodsmentioning

confidence: 99%

Sequestration and Elimination of Toxic Aldehydes

Nagasawa¹,

Valentekovich²,

Nagasawa³

et al. 2020

Chem. Res. Toxicol.

View full text Add to dashboard Cite

It is well-known that aldehydes resulting from the in vivo oxidation of primary alcohols are toxic. Here, we experimentally demonstrate in rat models that the dipeptide cysteinylglycine (CG), formed in vivo from its oxidized product, cystinyl-bis-glycine (CbG), will sequester acetaldehyde and isoamyl aldehyde, two model aldehydes resulting from the oxidation of ethanol and isoamyl alcohol, respectively, and excrete them in urine as their respective conjugation products with CG. These data suggest that a whole series of toxic aldehydes can be sequestered and detoxified by CG and may prevent the flushing syndrome exhibited by individuals with a defective enzyme that converts acetaldehyde to acetate. The data also suggest the possibility of alleviating the hangover syndrome we believe to be caused by aldehydes, such as isoamyl aldehyde derived from short, branched-chain alcohols, present as congeners in certain alcoholic beverages. The sequestration of other toxic agents, such as cyanide, that can react with CG can also be envisioned.

show abstract

Paper Chromatography of Glutathione and Its Hydrolysis Products

Cited by 10 publications

References 4 publications

DECOMPOSITION OF NATIVE KERATIN BY STREPTOMYCES FRADIAE

DECOMPOSITION OF NATIVE KERATIN BY STREPTOMYCES FRADIAE

Glutathione in Escherichia coli is dispensable for resistance to H2O2 and gamma radiation

Sequestration and Elimination of Toxic Aldehydes

Contact Info

Product

Resources

About