Paper Chromatography Using Capillary Ascent

Williams, Roger J.; Kirby, Helen

doi:10.1126/science.107.2784.481

Cited by 344 publications

(60 citation statements)

References 7 publications

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“…For descending solvent movement air-tight galvanized iron tanlcs were used, the filter paper being suspended from stainless steel troughs. For ascending solvent migration cylindrical glass museum jars were used in the manner of Williams and Kirby (1948). Whatman No.…”

Section: Methodsmentioning

confidence: 99%

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The Amino‐acid Composition of Certain Seed Proteins

Holmes¹

1953

Aust J Exp Biol Med

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A survey of the literature based on the accumulated data of Block and Boiling (1951) indicates that knowledge of the amino-acid composition of the proteins of legume seeds is limited. There is an almost complete lack of comprehensive data for the seeds of legumes of particular importance to Australian agriculture, such as lupins, vetches, peas and subterranean clover. Investigations of the digestibility and biological value of the protein of some of these seeds, using nitrogen balance methods, have been reported from this laboratory for the growth of sheep (Williams and Moir, 1951) and for the growth of the rat (Smythe, 1950) but they were not supported by any extensive data on their amino-acid composition. The arginine content of these seed proteins was, however, reported by the author (Holmes, 1951) and the cyst(e)ine and methionine contents of some of them by Johanson (1948).In recent years considerable attention has been devoted to the sulphurcontaining amino-acids in seed proteins (Lugg, 1945 and 194C; Johanson and Lugg, 1946). As a result it has beeii establislied that legume seeds and their bulk proteins are of very low or low methionine content and their cyst(e)ine values range from very low to moderately high. Numerous feeding trials indicate that the methionine contents of legume seeds may constitute a first limiting factor in nutrition, at least in the rat and the chick.' On the other hand, in spite of the not unexpected emphasis placed on the sulphur-containing amino-acids in wool growth (Marston, 1935) no correlation could be found by Johanson (loc. cit.) between cyst(e)ine + methionine fed and wool growth in the series of feeding trials carried out by Stewart and Moir (1947). In these trials marked differences were found between different protein sources, including several legume seeds, in promoting wool growth in Merino sheep when these proteins were fed at the same overall level of nitrogen intake and constituted almost the whole of the nitrogen of otherwise similar rations. The possibility of other essential amino-acids limiting the wool-growing capacity of these proteins, particularly those occurring in wool keratin in high proportions,, namely arginine, leucine and threonine, could not be excluded. The present study of the amino-acid composition of certain legume seed proteins and of linseed protein.

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Section: Methodsmentioning

confidence: 99%

“…4 filter paper was used throughout. Phenol was purified by the method of Williams and Kirby (1948), coUidine by the method of Partridge (1948) and alcohols by fractional distillation. Water saturated solvents were allowed to come to equilibrium until clear solutions were obtained at the chromatographic temperature.…”

Section: Methodsmentioning

confidence: 99%

The Amino‐acid Composition of Certain Seed Proteins

Holmes¹

1953

Aust J Exp Biol Med

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“…(e) Chromatographic Technique (i) Solvents.-The ascending method of Williams and Kirby (1948) was used as modified by Wolfson, Cohn, and Devaney (1949). For two-dimensional chromatograms 80 per cent.…”

Section: (D) Protein Hydrolysismentioning

confidence: 99%

The Physiology of Growth in Apple Fruits V. Soluble Nitrogen Constituents

McKee¹,

Urbach²

1953

Aust. Jnl. Of Bio. Sci.

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SummaryThe ninhydrin-reacting compounds of the apple fruit have been studied during its development on the tree. The compounds present show little variation, but glutamine, conspicuous in very young and in over-mature apples, disappears at intermediate stages. The increase in soluble nitrogenous compounds occurring in over-mature apples left on the tree (Pearson and Robertson 1953) is confirmed qualitatively. An account is given of the chromatographic behaviour of several unidentified ninhydrin-reacting substances found in extracts of apple fruits, leaves, and stems.

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“…An apparatus for the partition chromatography method using paper was designed after suggestions of Consden et al (10) and Goodall and Levi (9). The method of paper chromatography using ascending flow, described by Williams and Kirby (11), was adapted to the work discussed here by using the butanol-15% acetic acid-water solvent system and developing in one dimension only. Both intact materials and hydrolyzates were similarly analyzed on paper (12).…”

Section: Methodsmentioning

confidence: 99%

Chemical Studies on Polymyxin B 1

Regna

Solomons²,

Forscher³

et al. 1949

J. Clin. Invest.

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Polymyxin is a generic term for chemotherapeutic antibiotics elaborated in fermentations of various media by strains of Bacillus polymyxa (1). The known polymyxin polypeptides (A, B, C, D, and E) have closely similar chemical and biological properties, and have in common L-a, y-diaminobutyric acid, L-threonine and an unidentified C9 optically-active fatty acid. In addition to these acids, polymyxin A (aerosporin) contains D-leucine (2). Polymyxin A, first described by Ainsworth, Brown and Brownlee, was isolated by Brownlee and Bushby, and Catch and Friedmann (3) from broth cultures by adsorption onto a suitable carbon and elution with aqueous acetone containing sulfuric acid. Further purification was obtained by converting the antibiotic to the helianthate and subsequently to the hydrochloride. Polymyxin D, which is composed of the constituents of polymyxin A plus serine, was described by Benedict and Langlykke (4) and During the course of studies on polymyxin B designed to eliminate toxic impurities and prepare high potency material, a procedure was developed involving adsorption of the active principle on cotton sodium succinate, elution with dilute sulfuric acid, precipitation of the antibiotic with 1-(4-chloro-o-sulfophenyl)-5-hydroxy-3-1

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Paper Chromatography Using Capillary Ascent

Cited by 344 publications

References 7 publications

The Amino‐acid Composition of Certain Seed Proteins

The Amino‐acid Composition of Certain Seed Proteins

The Physiology of Growth in Apple Fruits V. Soluble Nitrogen Constituents

Chemical Studies on Polymyxin B 1

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