Paper Spray Tandem Mass Spectrometry for Assessing Oleic, Linoleic and Linolenic Acid Content in Edible Vegetable Oils

Abstract

Oleic, linoleic and linolenic acids exert several beneficial effects on human health, some of which are also certified by recent European and U.S. regulations. The goal of the presented work was to develop an innovative methodology to evaluate their content in edible vegetable oils, in order to increase the value of oils from a nutraceutical perspective. The protocol is based on the use of paper spray ionization coupled with tandem mass spectrometry experiments, which allowed the recording of data very quickly and with high specificity. All investigated compounds gained a good linear relation (r2 higher than 0.98). Accuracy values are near 100% for all concentration levels examined, and the repeatability and reproducibility data result lower than 15%, highlighting the consistence of the methodology. The developed approach was successfully applied for the analysis of different real samples, and its robustness was confirmed by comparing the results obtained with those coming from the classical and official methodology.

Rapid and simultaneous determination of curcuminoids and gingerols in food products containing turmeric and ginger using paper spray mass spectrometry

“…Paper spray mass spectrometry has found widespread application in many areas; in food analysis, for example, it has already been used to carry out molecular screening and quantification of several nutraceutical compounds for assessing products quality and authenticity. [26][27][28][29][30][31][32][33] Furthermore, it has been demonstrated that paper spray ionization may provide comprehensive MS profiles by detecting a large number of molecules, comparable to those achieved by direct infusion analysis. With respect to other commonly used ambient sources, PS-MS seems to be excellent for the analysis of polar compounds, such as organic acids and phenols in food matrices.…”

“…These latter are transported and released into an ionized spray by the simultaneous application of a polar solvent and a high voltage to the paper. Paper spray mass spectrometry has found widespread application in many areas; in food analysis, for example, it has already been used to carry out molecular screening and quantification of several nutraceutical compounds for assessing products quality and authenticity 26–33 . Furthermore, it has been demonstrated that paper spray ionization may provide comprehensive MS profiles by detecting a large number of molecules, comparable to those achieved by direct infusion analysis.…”

Cymbopogon citratus Water Extract and Methyl Jasmonate Improve Polyunsaturated Fatty Acid Metabolism in Taiwanofungus camphoratus Mycelia

“…The great variety of edible mushrooms found worldwide and their consistent production of fatty acids, regardless of the geographic source, make them an important source of essential fatty acids within a healthy human diet. Their lipid profiles include essential fatty acids such as the linoleic acids (LAs), oleic acids (OAs), and linolenic acids, which act against cardiovascular diseases [36]. LA is a PUFA that has anti-tumor activity against cytotoxic T lymphocytes (CD8+T) cells in vitro and in vivo and can be considered an enhancer of adoptive T cell therapy in cancer therapy [37].…”