Papillary Architecture and Functional Characterization of Mucosubstances in the Sheep Tongue

Abstract

This research aimed to reveal the general morphology and topographic distribution of lingual papillae, epithelial characteristics, mucosal structure, and glands with their mucin content in the sheep tongue, with consideration of species-specific characteristics. The tongues of ten sheep were analyzed for this purpose. Filiform and fungiform papillae existed within the borders of the ventral surface of the lingual apex. The majority of the filiform papillae had multiple secondary projections. Fungiform papillae were also seen on the lingual torus among lenticular papillae, as well as 6 to 10 circumvallate papillae arranged on its caudal border. The species-specific details of the general anatomical structure of the tongue were determined and, in general, the papillary organization in the sheep was similar to goats, while the papillary organization also was similar to features with deer species, specifically the filiform papilla from the mechanical papillae and fungiform papilla from the gustatory papillae. Neutral and weak sulfated mucins and N-acetyl sialomucins were located in seromucous glands, salivary duct epithelium and von Ebner's glands. Carboxylated acid mucins and N-acetyl sialomucins were not present in seromucous and von Ebner's glands. In seromucous glands, MUC1, MUC5AC and MUC6 localized only in epithelial cells of ducts, whereas MUC2 localized in both glandular and ductal epithelial cells. All MUCs were present in both von Ebner's glands and salivary ducts. We showed that this mucin composition, may serve as a physical barrier in the initial section of the digestive system. This article is protected by copyright. All rights reserved.

Morphology of the Atlantic salmon (Salmo salar)tongue

“…Morphological data, particularly on tongue of different species of fish, are present in literature (Abbate et al., 2006, 2017; Abbate, Guerrera, Montalbano, Ciriaco, & Germanà, 2012; Abbate, Guerrera, Montalbano, De Carlos, et al, 2012; Collar, O'Meara, Wainwright, & Near, 2009; Dos Santos, Arantes, Santiago, & Santos, 2015; El Bakary, 2014; Elgendy, Alsafy, & Tanekhy, 2016; Guerrera et al., 2015; Ikpegbu, Ibe, & Nlebedum, 2019; Levanti et al., 2017; Mahmoud, Essa, & Sayed, 2016; Montalbano et al, 2016; Price et al., 2010; Sadeghinezhad, Rahmati‐Holasoo, Fayyaz, & Zargar, 2015; Yashpal, Kumari, Mittal, & Mittal, 2009). Nevertheless, recent data regarding the tongue anatomy in upper vertebrates are significative and could be very important in comparative anatomy (Abbate et al., 2008, 2009, ; Emura, 2016a, 2016b, 2016c, 2017, 2018a, 2018b, 2018c, 2018d; Emura & Sugiyama, 2016; Erdogan & Alan, 2012; Erdoğan & Iwasaki, 2014; Erdoğan, Lima, & Pérez, 2016; Erdoğan & Sağsöz, 2018; Erdoğan, Villar Arias, & Pérez, 2016; Erdoğan, Villar, König, & Pérez, 2018; Herrel, Redding, Meyers, & Nishikawa, 2014; Jackowiak, Skieresz‐Szewczyk, Godynicki, Iwasaki, & Meyer, 2011; Jackowiak, Skieresz‐Szewczyk, Kwiecińsk, Trzcielińska‐Lorych, & Godynicki, 2010; Sadeghinezhad et al., 2015; Sadeghinezhad, Sheibani, Memarian, & Chiocchetti, 2017; Sadeghinezhad, Tootian, & Javadi, 2018; Skieresz‐Szewczyk, Cornillie, & Jackowiak, 2018; Skieresz‐Szewczyk & Jackowiak, 2016, 2017; Skieresz‐Szewczyk, Jackowiak, & Ratajczak, 2018, 2019). The objective of this study is to investigate, by light, laser confocal and scanning electron microscopy, the morphological characteristics of the tongue, considering the importance of the correlations between feeding habits and...…”

“…Morphological data, particularly on tongue of different species of fish, are present in literature (Abbate et al, 2006(Abbate et al, , 2017Abbate, Guerrera, Montalbano, Ciriaco, & Germanà, 2012; Abbate, Guerrera, Montalbano, De Carlos, et al, 2012;Collar, O'Meara, Wainwright, & Near, 2009;Dos Santos, Arantes, Santiago, & Santos, 2015;El Bakary, 2014;Elgendy, Alsafy, & Tanekhy, 2016;Guerrera et al, 2015;Ikpegbu, Ibe, & Nlebedum, 2019;Levanti et al, 2017;Mahmoud, Essa, & Sayed, 2016;Montalbano et al, 2016;Price et al, 2010;Sadeghinezhad, Rahmati-Holasoo, Fayyaz, & Zargar, 2015;Yashpal, Kumari, Mittal, & Mittal, 2009). Nevertheless, recent data regarding the tongue anatomy in upper vertebrates are significative and could be very important in comparative anatomy (Abbate et al, 2008(Abbate et al, , 2009Emura, 2016aEmura, , 2016bEmura, , 2016cEmura, , 2017Emura, , 2018aEmura, , 2018bEmura, , 2018cEmura, , 2018dEmura & Sugiyama, 2016;Erdogan & Alan, 2012;Erdoğan & Iwasaki, 2014;Erdoğan, Lima, & Pérez, 2016;Erdoğan & Sağsöz, 2018;Erdoğan, Villar Arias, & Pérez, 2016;Erdoğan, Villar, König, & Pérez, 2018;Herrel, Redding, Meyers, & Nishikawa, 2014;Jackowiak, Skieresz-Szewczyk, Godynicki, Iwasaki, & Meyer, 2011;…”

Anatomical, histological and immunohistochemical study of the tongue in the rainbow trout (Oncorhynchus mykiss)

“…The most of the functional processes inside the oral cavity are carried out by the tongue, whose presence and description have been showed in different species of fish, demonstrating the variation in the presence of anatomical structures like taste buds, mucous cells and teeth in teleosts, also of commercial interest (Abbate et al., 2006,2017,2020a,2020b; Abbate, Guerrera, Montalbano, Ciriaco, et al., 2012; Abbate, Guerrera, Montalbano, De Carlos, et al., 2012; Alsafy, Bassuoni, & Hanafy, 2018; Amato et al., 2012; Dos Santos, Arantes, Santiago, & Dos Santos, 2015; El Bakary, 2014; Elgendy, Alsafy, & Tanekhy, 2016; Germanà et al., 2009; Guerrera et al., 2015; Ikpegbu, Ibe, & Nlebedum, 2019; Kasumyan, 2019; Kettratad, Senarat, Boonyoung, & Jiraungkoorsku, 2017; Levanti et al., 2017; Mahmoud, Essa, & Sayed, 2016; Sadeghinezhad, Rahmati‐holasoo, Fayyaz, & Zargar, 2015; Sayed, Mahmoud, & Essa, 2019; Yu et al., 2019). Several significant papers, important as comparative data, are present in the literature in upper vertebrates like birds, reptiles and mammals (Abbate et al, 2008, 2009, 2010, 2020b; Abumandour & Kandyel, 2020; Barbosa et al., 2020; Bels et al., 2020; Cizek, Hamouzova, Goździewska‐Harłajczuk, Klećkowska‐Nawrot, & Kvapil, 2020; Cizek, Hamouzova, Kvapil, & Kyllar, 2019; Emura, 2016a,2016b,2016c,2017,2018a,2018b,2018c,2018d,2019; Erdoğan & Alan, 2012; Erdoğan & Iwasaki, 2014; Erdoğan Lima & Pérez, 2016; Erdoğan Villar Arias & Pérez, 2016; Erdoğan, Villar Arias, et al., 2016; Erdoğan et al., 2018a; Erdoğan and Sağsöz, 2018b; Freire et al., 2019; Gonçalves et al., …”

Microstructure of the Surface of the Tongue and Histochemical Study of the Lingual Glands of the Lowland Tapir (Tapirus terrestris Linnaeus, 1758) (Perissodactyla: Tapiridae)

“…Histochemical analysis of the lingual glands of lowland tapir showed the similarity especially of von Ebner's glands secretion to the same glands in sheep [49]. On the other hand, there were differences in secretion nature of the mucoserous glands of the root of tongue between lowland tapir and sheep [49]. However, the mucous units of posterior lingual glands were dominant both in sheep [49] as well as in examined lowland tapir.…”

“…The number and shape of the vallate papillae differs between the lowland tapir and the Artiodactyla, where there are from 1 to 5 vallate papillae on both sides of the caudolateral aspect of the tongue [45] in the alpaca, 26 vallate papillae [42] in the goitered gazelle, and 6-8 on both sides of the caudolateral aspect in the bactrian camel [47]. Histochemical analysis of the lingual glands of lowland tapir showed the similarity especially of von Ebner's glands secretion to the same glands in sheep [49]. On the other hand, there were differences in secretion nature of the mucoserous glands of the root of tongue between lowland tapir and sheep [49].…”