Para amino benzoic acid-derived self-assembled biocompatible nanoparticles for efficient delivery of siRNA

Abstract

A number of diseases can result from abnormal gene expression. One of the approaches for treating such diseases is gene therapy to inhibit expression of a particular gene in a specific cell population by RNA interference. Use of efficient delivery vehicles increases the safety and success of gene therapy. Here we report the development of functionalized biocompatible fluorescent nanoparticles from para amino benzoic acid nanoparticles for efficient delivery of short interfering RNA (siRNA). These nanoparticles were non-toxic and did not interfere with progression of the cell cycle. The intrinsic fluorescent nature of these nanoparticles allows easy tracking and an opportunity for diagnostic applications. Human Bcl-2 siRNA was complexed with these nanoparticles to inhibit expression in cells at both the transcriptional and translational levels. Our findings indicated high gene transfection efficiency. These biocompatible nanoparticles allow targeted delivery of siRNA, providing an efficient vehicle for gene delivery.

Simultaneous delivery of Paclitaxel and Bcl-2 siRNA via pH-Sensitive liposomal nanocarrier for the synergistic treatment of melanoma

“…Several materials including polymeric micelles7, dendrimers8, various nanoparticles9 and liposomes10 are being used in drug delivery systems. Research over a period of time has shown the delivery of small molecule therapeutics and its subsequent release in response to external stimuli such as temperature, light1112, redox reagents13, ultrasound10, pH, enzymes, etc.…”