Parabiosis Between Avian Embryos Resistant and Susceptible to Rous Sarcoma Virus

Abstract

Parabiosis of chicken embryos was used to determine if resistance or susceptibility to Rous sarcoma virus (RSV) tumors could be transferred between RSV-resistant (R) and RSV-susceptible (S) embryos. Eggs from RSV-R and RSV-S lines were parabiosed at 12 days of incubation. One week after hatching the chicks were inoculated in the wing web with RSV. Birds were examined for tumor lesions through 9 weeks of age. Lesion scores of the nonparabiosed RSV-S line were significantly higher than nonparabiosed RSV-R lines. Lesion scores of RSV-R chicks that had been parabiosed to RSV-S chicks were higher than nonparabiosed RSV-R chicks. Lesion scores of RSV-S chicks parabiosed to RSV-R chicks were not different from nonparabiosed RSV-S chicks. These results suggest that the resistance to RSV was not transferred between parabionts but that there was significant transfer of susceptibility from the RSV-S embryos to the RSV-R embryos.