Human motion analysis is one of the important research directions in computer vision technology. Aiming at the problems of poor effect, accuracy, and efficiency in the current tennis serve trajectory capture process, a tennis serve trajectory capture algorithm based on wavelet multiscale decomposition is proposed. This article analyzes the related concepts and principles of wavelet transform and multiresolution analysis, uses the computer three-dimensional vision acquisition method to collect the tennis serve trajectory image, and uses the mean filter to smooth and denoise the collected tennis serve trajectory image. The wavelet multiscale decomposition algorithm is used to process the tennis serve trajectory image after smooth denoising, find the local maximum point through the modulus value and phase angle value, and obtain the initial tennis serve trajectory image. Using the local layer threshold method, the adaptive threshold is set, and the trajectory line under each scale is obtained to capture the trajectory line of tennis serve. It can be observed from the experimental and simulation results that the trajectory capture effect of the proposed algorithm is good, and it can effectively improve the accuracy and efficiency of trajectory capture.