Paracnuella aquatica gen. nov., sp. nov., a member of the family Chitinophagaceae isolated from a hot spring

Paraflavisolibacter caeni gen. nov., sp. nov., a novel taxon within the family Chitinophagaceae isolated from sludge

“…The major fatty acids of strain LB-8 T (≥10 %) were iso-C 15 : 0 (25.3 %), anteiso-C 15 : 0 (16.9 %), iso-C 17 : 0 3-OH (10.0 %) and summed feature 3 (C 16 : 1 ω7c and/or C 16 : 1 ω6c, 11.6 %). Among them, the percentage proportions of iso-C 15 : 0 and iso-C 17 : 0 3-OH were consistent with those of the related type strains of the family Chitinophagaceae [6,7,[29][30][31] (Table 2). However, strain LB-8 T could be differentiated from its near relatives by producing large amount of anteiso-C 15 : 0 and failing to produce iso-C 15 : 1 G. Compared to its closest relative F. tropicus LCS9 T , iso-C 15 : 1 G and anteiso-C 15 : 1 A were the major fatty acids in LCS9 T , but not detected in strain LB-8 T (Table 2).…”

“…However, strain LB-8 T could be differentiated from its near relatives by producing large amount of anteiso-C 15 : 0 and failing to produce iso-C 15 : 1 G. Compared to its closest relative F. tropicus LCS9 T , iso-C 15 : 1 G and anteiso-C 15 : 1 A were the major fatty acids in LCS9 T , but not detected in strain LB-8 T (Table 2). The only respiratory quinone of strain LB-8 T was menaquinone-7 (MK-7), which is also reported for the genera Flavisolibacter, Paracnuella, Cnuella and other members of the family Chitinophagaceae [1,[4][5][6][7][8][29][30][31].…”

“…Strain LB-8 T was sensitive to erythromycin (15 µg), lincomycin (2 µg), streptomycin (10 µg), chloramphenicol (30 µg), rifampicin (5 µg), ofloxacin (5 µg), novobiocin (30 µg), cefradine (10 µg), clindamycin (10 µg), vancomycin (15 µg) and tetracycline (30 µg), while resistant to gentamicin (10 µg), polymyxin B (30 µg), amoxicillin (10 µg), penicillin (10 µg), ampicillin (10 µg), kanamycin (15 µg), neomycin (30 µg) and spectinomycin (100 µg). Strain LB-8 T exhibited the common phenotypic characteristics of the family Chitinophagaceae , such as positive for activities of catalase, alkaline phosphatase, leucine arylamidase and valine arylamidase, and negative for oxidase, H 2 S and indole production [7]. Compared with the reference strains Flavisolibacter tropicus LCS9 T [29], Cnuella takakiae RG-1 T [6], Paracnuella aquatica N24 T [7] and Niastella populi KCTC 22560 T [30], strain LB-8 T did not hydrolyse gelatin, but assimilated potassium gluconate, adipic acid and malic acid ().…”

“…According to the List of Prokaryotic Names with Standing in Nomenclature (https://lpsn.dsmz.de/family/chitinophagaceae) [2], there are 53 validly published species (including synonyms) in the family Chitinophagaceae with Chitinophaga [3] as the type genus. Members of the family Chitinophagaceae are Gram-negative, aerobic or facultatively anaerobic, usually non-motile, rod-shaped and contain MK-7 as the isoprenoid quinone and iso-C 15 : 0 , iso-C 17 : 0 3-OH and iso-C 15 : 1 G as the major fatty acids [1, 3–8]. During the course of a study on the bacterial diversity in an activated sludge collected from an agricultural chemical factory in Binzhou city, Shandong province, PR China, a pale yellow-pigmented bacterium, named LB-8 T , was isolated.…”

The Role of Thermal Water in Chronic Skin Diseases Management: A Review of the Literature

“…In particular, a novel species of Desulfovibrio was isolated from the waters of a Tunisian thermal spring, proposed as Desulfovibrio biadhensis [ 74 ]. Moreover, from a radioactive thermal spring in Budapest, a novel species of the genus Deinococcus was isolated, named Deinococcus fonticola [ 83 ], and from thermal spring water sampled at Xi’an (PR China) a novel species of a new genus in the family Chitinophagaceae, phylum Bacteroidetes, for which the name Paracnuella aquatica was proposed [ 84 ].…”