Paracoccus chinensis sp. nov., isolated from sediment of a reservoir

Abstract

A Gram-negative, short ovoid-to coccus-shaped, aerobic, motile, non-spore-forming bacterium (designated strain KS-11 T ) was isolated from sediment of the eutrophic Guanting reservoir in Beijing, China. Colonies grown on R2A agar plates were circular, convex and colourless to orange. The strain grew in the presence of up to 1 % NaCl (optimum, 0 % NaCl). Growth occurred at 25-40 6C (optimum, 28-37 6C) and at pH 6.0-9.5 (optimum, pH 7.5-9.0). On the basis of 16S rRNA gene sequence similarity, strain KS-11 T was shown to belong to the class Alphaproteobacteria, being closely related to Paracoccus marinus (96.9 % 16S rRNA gene sequence similarity), followed by Paracoccus koreensis (96.8 %), Paracoccus solventivorans (96.8 %), Paracoccus alkenifer (96.2 %) and Paracoccus kocurii (95.8 %). The major fatty acids of strain KS-11 T were summed feature 7 (C 18 : 1 v7c/v9t/v12t) (83.8 %) and C 18 : 0 (6.5 %) and the G+C content of the genomic DNA was 69.0 mol%. Based on comparative analysis of physiological and chemotaxonomic data, it is proposed that strain KS-11 T represents a novel species of the genus Paracoccus, named Paracoccus chinensis sp. nov. The type strain is KS-11 T (5CGMCC 1.7655 T 5NBRC 104937 T ).The Guanting reservoir lies in the north-west of Beijing, China and has been eutrophic for several decades. Several novel bacterial strains have been isolated from the reservoir sediment (Qu & Yuan, 2008a;Qu et al., 2008b). One of the isolates, designated strain KS-11 T , was found to be a member of the genus Paracoccus in the Alphaproteobacteria according to the 16S rRNA gene sequence phylogenetic analysis.The genus Paracoccus was first reported by Davis et al. (1969). To date, the genus is composed of more than 20 species with validly published names. The Gram-negative, catalase-and oxidase-positive, coccus or short-rod members of this genus show a variety of metabolic properties. One species is able to grow methylotrophically with methyl compounds such as methylamine or N,N-dimethylformamide (Urakami et al., 1990). Another species can oxidize reduced sulfur compounds (Rainey et al., 1999), whereas some strains are able to reduce nitrate to N 2 under anaerobic conditions (Siller et al., 1996) Strain KS-11 T was isolated from surface sediment of the Guanting reservoir. The samples were suspended and serially diluted in 50 mM phosphate buffer (pH 7.0) and spread on R2A agar plates (containing, l 21 distilled water: 0.5 g glucose, 0.5 g soluble starch, 0.5 g casein hydrolysate, 0.5 g yeast extract, 0.5 g peptone, 0.05 g MgSO 4 . 7H 2 O, 0.3 g KH 2 PO 4 and 16 g agar, with the pH adjusted to 7.2 prior to autoclaving). After incubation at 28 u C for 3 weeks, well-separated colonies were picked randomly and purified by subculture on new plates. This procedure was performed several times. Strain KS-11 T comprised one of these colonies and was cultured routinely on R2A agar plates and maintained as a glycerol suspension (20 %, v/v) at 280 u C. The temperature range and optimum for growth were determined at 4, 20, 28, 3...

Paracoccus limosus sp. nov., isolated from activated sludge in a sewage treatment plant

“…Many novel species have been described in recent years from environments including soil, sediment and activated sludge, e.g. Paracoccus stylophorae (Sheu et al , 2011), P. chinensis (Li et al , 2009), P. aestuarii (Roh et al , 2009) and P. caeni (Lee et al , 2011).…”

Paracoccus rhizosphaerae sp. nov., isolated from the rhizosphere of the plant Crossostephium chinense (L.) Makino (Seremban)

“…At the time of writing, the genus Paracoccus comprised the following recognized species: P. aestuarii (Roh et al , 2009), P. alcaliphilus (Urakami et al , 1989), P. alkenifer (Lipski et al , 1998), P. aminophilus and P. aminovorans (Urakami et al , 1990), P. bengalensis (Ghosh et al , 2006), P. carotinifaciens (Tsubokura et al , 1999), P. chinensis (Li et al , 2009), P. denitrificans (Beijerinck & Minkman, 1910; Davis et al , 1969; Skerman et al , 1980 emend. Nokhal & Schlegel, 1983; Ludwig et al , 1993), P. fistulariae (Kim et al , 2010), P. haeundaensis (Lee et al , 2004), P. halodenitrificans (Robinson & Gibbons, 1952), P. halophilus (Liu et al , 2008), P. homiensis (Kim et al , 2006), P. isoporae (Chen et al , 2011), P. kocurii (Ohara et al , 1990), P. kondratievae (Doronina & Trotsenko, 2000 emend.…”

Rhizocarpon geographicumlichen discloses a highly diversified microbiota carrying antibiotic resistance and Persistent Organic Pollutants tolerance

“…Most of the species isolated from media reproducing the lichen environment are known to evolve in water reservoirs and polluted environments. For instance, Aquaspirillum arcticum, Erythrobacter sp, Klenkia taihuensis, Paracoccus chinensis, Microterricola gilva, and Kocuria polaris (Table 1), were previously isolated from marine environments [39][40][41][42], sediments [43,44], seaweed [45] or cyanobacteria [46]. It is to note that various Erythrobacter, Paracoccus, Kocuria, Pseudomonas or Gordonia species were already isolated from marine or maritime Brittany lichens and P. helmanticensis from an island Austrian lichen [42].…”