Paracoccus xiamenensis sp. nov., isolated from seawater on the Xiamen

Abstract

Strain 12-3T was isolated from seawater of the Guanyinshan Coast, Xiamen, Fujian Province, PR China. The bacterium was Gram-stain-negative, rod-shaped, aerobic, oxidase-positive and catalase-negative. Growth of strain 12-3T occurred at 10–37 °C (optimum, 20–30 °C), at pH 5.0–11.0 (optimum, pH 7.0–8.0) and at a salinity range of 0–10 % (optimum, 3–5 %). The results of phylogenetic analysis based on its 16S rRNA gene sequence indicated that strain 12-3T belonged to the genus Paracoccus and had the highest sequence similarity to Paracoccus lutimaris HDM-25T (97.4 %), followed by Paracoccus isoporae SW-3T (96.9 %), Paracoccus caeni MJ17T (96.9 %), Paracoccus pacificus F14T (96.8 %) and other species in the genus Paracoccus (95.3–96.5 %). The average nucleotide identity (ANI) and DNA–DNA hybridization (DDH) values between strain 12-3T and P. lutimaris HDM-25T were 76.1 and 17.0 %, respectively. ANI and DDH values between strain 12-3T and P. isoporae SW-3T were 78.9 and 18.2 %, respectively. The principal fatty acid of strain 12-3T was summed feature 8 (C18 : 1 ω6c/ω7c) and C18 : 0. The respiratory quinone of strain 12-3T was Q10. The polar lipids included phosphatidylcholine, phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol and an unidentified glycolipid. The G+C content of the chromosomal DNA was 63.9 mol%. The combination of the results of the phylogenetic, phenotypic and chemotaxonomic analyses, and its low ANI and DDH values indicate that strain 12-3T represents a novel species of the genus Paracoccus , for which the name Paracoccus xiamenensis sp. nov. is proposed. The type strain is 12-3T (=MCCC 1A16381T=KCTC 72687T).

Paracoccus amoyensis sp. nov., isolated from the surface seawater along the coast of Xiamen Island, China

“…*Data taken from: a Lee et al . (2011) [17]; b Chen et al . (2017) [19]; c Lyu, Lina et al (2020) [18]; d Zhang et al .…”

“…However, PE was determined in strain 11-3 T , P. xiamenensis 12-3 T [18], P. caeni MJ17 T [46] and P. denitrificans DSM 413 T [37], not in P. pacificus F14 T [10]. GL was found in strain 11-3 T , P. xiamenensis 12-3 T [18] and P. caeni MJ17 T [46], not in P. pacificus F14 T [10] and P. denitrificans DSM 413 T [37]. The respiratory quinones of strain 11-3 T were Q9 (1 %) and Q10 (99 %), and the major component of Q10 was consistent with that in other reference strains.…”

“…xiamenensis 12-3 T (66.9 mol%) and P. pacificus F14 T (61.4 mol%), with their respective differences of 1.4, 6.2, 6.8 and 1.3 % [1–10, 10–18, 37]. Based on differences <1.0 % (cut-off value for defining a new species), strain 11-3 T can be considered as a new species [38].…”

“…S4). Among them, DPG, PG and PC were also present in other strains of genus Paracoccus [17, 32, 46]. However, PE was determined in strain 11-3 T , P.…”

“…The purity of strain 11-3 T was confirmed by uniformity of cell morphology and 16S rRNA gene sequence. In this study, four type strains of Paracoccus caeni MJ17 T [17], Paracoccus xiamenensis 12-3 T [18], Paracoccus pacificus F14 T [10] and Paracoccus denitrificans DSM 413 T [19] were used as reference strains for phenotypic and fatty acid analysis. During the study, strain 11-3 T and reference strains were cultured on MA plates or in marine broth 2216 (MB, BD Difco) at 28 °C unless where indicated.…”