Paracrine and endocrine functions of osteocytes

Michigami, Toshimi

doi:10.1297/cpe.2022-0053

Cited by 3 publications

(2 citation statements)

References 99 publications

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“…In mature bone tissue, OCYs account for about 90% to 95% of all bone tissue cells, and they are also the longest-living cells in bone [25,26]. However, previous research on bone tissue has primarily focused on OBs due to the fact that OCYs are deeply embedded in hard bone tissue and extremely difficult to isolate and extract [27]. This article describes the initial isolation and culture of primary OCYs, where the identification of primary OCYs was based on cell morphology with slender synapses, E11-specific protein expression, and weak ALP expression.…”

Section: Discussionmentioning

confidence: 99%

C-C Motif Chemokine Ligand 5 (CCL5) Promotes Irradiation-Evoked Osteoclastogenesis

Wang,

Zhao,

et al. 2023

IJMS

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The imbalance that occurs in bone remodeling induced by irradiation (IR) is the disruption of the balance between bone formation and bone resorption. In this study, primary osteocytes (OCYs) of femoral and tibial origin were cultured and irradiated. It was observed that irradiated OCY showed extensive DNA damage, which led to the initiation of a typical phenotype of cellular senescence, including the secretion of senescence-associated secretory phenotype (SASP), especially the C-C motif chemokine ligand 5 (CCL5). In order to explore the regulation of osteoclastogenic potential by IR-induced senescent OCYs exocytosis factor CCL5, the conditioned medium (CM) of OCYs was co-cultured with RAW264.7 precursor cells. It was observed that in the irradiated OCY co-cultured group, the migration potential increased compared with the vehicle culture group, accompanied by an enhancement of typical mature OCs; the expression of the specific function of enzyme tartrate-resistant acid phosphatase (TRAP) increased; and the bone-destructive function was enhanced. However, a neutralizing antibody to CCL5 could reverse the extra-activation of osteoclastogenesis. Accordingly, the overexpression of p-STAT3 in irradiated OCY was accompanied by CCL5. It was concluded that CCL5 is a potential key molecule and the interventions targeting CCL5 could be a potential strategy for inhibiting osteoclastogenesis and restoring bone remodeling.

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Section: Discussionmentioning

confidence: 99%

C-C Motif Chemokine Ligand 5 (CCL5) Promotes Irradiation-Evoked Osteoclastogenesis

Wang,

Zhao,

et al. 2023

IJMS

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“…[8,[11][12][13][14][15][16] The gut microbiome may impact osteocytes directly by changing paracrine and endocrine signaling from trafficking immune cells. [17] However, whether the microbiome has sexually dimorphic effects on bone cells, bone tissue metabolism, and multiscale bone quality is still uncertain; therefore, important interactions between the gut and the skeleton may be masked.…”

Section: Introductionmentioning

confidence: 99%

Germ‐Free C57BL/6 Mice Have Increased Bone Mass and Altered Matrix Properties but Not Decreased Bone Fracture Resistance

Vahidi

Moody

Welhaven

et al. 2023

J of Bone & Mineral Res

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The gut microbiome impacts bone mass, which implies a disruption to bone homeostasis. However, it is not yet clear how the gut microbiome affects the regulation of bone mass and bone quality. We hypothesized that germ‐free (GF) mice have increased bone mass and decreased bone toughness compared with conventionally housed mice. We tested this hypothesis using adult (20‐ to 21‐week‐old) C57BL/6J GF and conventionally raised female and male mice (n = 6–10/group). Trabecular microarchitecture and cortical geometry were measured from micro–CT of the femur distal metaphysis and cortical midshaft. Whole‐femur strength and estimated material properties were measured using three‐point bending and notched fracture toughness. Bone matrix properties were measured for the cortical femur by quantitative back‐scattered electron imaging and nanoindentation, and, for the humerus, by Raman spectroscopy and fluorescent advanced glycation end product (fAGE) assay. Shifts in cortical tissue metabolism were measured from the contralateral humerus. GF mice had reduced bone resorption, increased trabecular bone microarchitecture, increased tissue strength and decreased whole‐bone strength that was not explained by differences in bone size, increased tissue mineralization and fAGEs, and altered collagen structure that did not decrease fracture toughness. We observed several sex differences in GF mice, most notably for bone tissue metabolism. Male GF mice had a greater signature of amino acid metabolism, and female GF mice had a greater signature of lipid metabolism, exceeding the metabolic sex differences of the conventional mice. Together, these data demonstrate that the GF state in C57BL/6J mice alters bone mass and matrix properties but does not decrease bone fracture resistance. © 2023 The Authors. Journal of Bone and Mineral Research published by Wiley Periodicals LLC on behalf of American Society for Bone and Mineral Research (ASBMR).

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High-Throughput Preosteoblastic Spheroids Elevate Fibroblast Growth Factor 23 via Parathyroid Hormone Signaling Pathway

Jiang,

Zhu,

Lin

et al. 2024

Tissue Engineering Part C: Methods

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Paracrine and endocrine functions of osteocytes

Abstract: Highlightsl Osteocytes embedded in bone matrix produce paracrine and endocrine factors.l Osteocytes control bone mass through the secretion of sclerostin and RANKL.l Osteocytes play a central role in phosphate metabolism by producing FGF23.

Cited by 3 publications

References 99 publications

C-C Motif Chemokine Ligand 5 (CCL5) Promotes Irradiation-Evoked Osteoclastogenesis

C-C Motif Chemokine Ligand 5 (CCL5) Promotes Irradiation-Evoked Osteoclastogenesis

Germ‐Free C57BL/6 Mice Have Increased Bone Mass and Altered Matrix Properties but Not Decreased Bone Fracture Resistance

High-Throughput Preosteoblastic Spheroids Elevate Fibroblast Growth Factor 23 via Parathyroid Hormone Signaling Pathway

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