Paradoxical effects of interleukin‐10 on the maturation of murine myeloid dendritic cells

Commeren, Dianne L.; Soest, P. L. Van; Karimi, Khalil; Löwenberg, Bob; Cornelissen, Jan J.; Braakman, Eric

doi:10.1046/j.1365-2567.2003.01730.x

Cited by 26 publications

(21 citation statements)

References 28 publications

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“…As described in other viral infections, IFN-α and TNF-α expressions by pDC could have an inhibitory effect in IL-12 expression by cDC becoming unable to induce efficient cellular immune responses [2]. IL-10 expression in cDC was also shown to stimulate the inhibition of both DC subset functions [17,46].…”

Section: Discussionmentioning

confidence: 94%

Classical swine fever virus induces activation of plasmacytoid and conventional dendritic cells in tonsil, blood, and spleen of infected pigs

et al. 2007

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-Classical swine fever virus (CSFV) compromises the host immune system, causing indirect leucopoenia and disruption of in vitro T cell stimulation capacity. In order to explore the potential role of dendritic cells (DC) in such phenomena, the activation of conventional DC (cDC) and plasmacytoid DC (pDC) in blood and secondary lymphoid organs of infected pigs was investigated in the early time course post-inoculation (pi), together with viral components dissemination and cytokine production in serum. Whereas CD11R1 + CD172a + cDC frequencies were markedly reduced in blood and spleen, analysis of CD4 + CD172a + pDC numbers revealed a rapid turn-over of this DC subset in tissues pi. Both subsets matured and were activated after infection, as demonstrated by down-regulation of CD1a, up-regulation of the co-stimulation molecule CD80/86 and expression of cytokines. cDC essentially expressed tumor necrosis factor alpha (TNF-α) and interleukin (IL)-10, whereas pDC produced alpha interferon (IFN-α) and IL-12. IFN-α and TNF-α productions revealed an enhancement of innate anti-viral immune responses. Detection of antigen activated B lymphocytes in tonsil T-cell areas at 72 h pi, subsequently to the transient translocation of the viral E2 protein within germinal centres at 48 h pi, indicates the initiation of humoral response. This response was also evidenced by an important IL-10 production in serum one week pi. IL-12 expression in organs, as well as transient detection of IL-18 and IFN-γ in serum, reflected the initiation of cellular immune responses. However, the uncommonly high levels of TNF-α and IFN-α produced by DC and measured in serum early post-infection, together with IL-10 expression in spleen, could play a role in the disruption of immune system cells, either inducing apoptosis or impairing DC functionalities themselves.antigen presenting cells / secondary lymphoid organs / interferon / E2 antigen / pestivirus

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Section: Discussionmentioning

confidence: 94%

Classical swine fever virus induces activation of plasmacytoid and conventional dendritic cells in tonsil, blood, and spleen of infected pigs

et al. 2007

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“…IL-10 inhibits DC maturation and differentiation from monocyte precursors [38]. Thus, immuno-inhibitory properties of IL-10 is mainly due to their effect on antigen-presenting cells (APC) to prevent production of Th1 and Th2-associated cytokines [39].…”

Section: Introductionmentioning

confidence: 99%

Role of interleukin-10 in breast cancer

Ullah

Changkija

Konwar

2011

Breast Cancer Res Treat

155

113

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Cytokines are low molecular weight regulatory proteins or glycoprotein that modulates the intensity and duration of immune response by stimulating or inhibiting the activation, proliferation, and/or differentiation of target cells. Different cytokines are known to have diverse role in breast cancer initiation and progression. Interleukin-10 (IL-10), a pleiotropic anti-inflammatory cytokine, induces immunosuppression and assists in escape from tumor immune surveillance. Like several other cytokines, IL-10 also can exert dual proliferative and inhibitory effect on breast tumor cells indicating a complex role of IL-10 in breast cancer initiation and progression. In this review, we tried to put together a comprehensive current view on significance of IL-10 in promotion, inhibition, and importance as prognosticator in breast cancer based on in vitro, in vivo, and clinical evidences. For literature collection, we conducted PubMed search with keywords "IL-10" and "breast cancer".

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“…Whereas splenic DC treated with IL-10 induce a Th2 response when injected into mice, antigen presentation is generally impaired by IL-10-treated bone marrow-derived dendritic cells (BMDC) [6, 9,12,13,14]. In an ovalbumin (OVA) T-cell receptor transgenic mouse model, IL-10-treated BMDC also inhibit delayed type hypersensitivity reactions in the footpads of prophylactically or therapeutically vaccinated animals [15].…”

Section: Introductionmentioning

confidence: 99%

Interleukin-10-Treated Dendritic Cells Do Not Inhibit Th2 Immune Responses in Ovalbumin/Alum-Sensitized Mice

Bellinghausen¹,

Sudowe²,

König³

et al. 2006

Int Arch Allergy Immunol

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Background: It is well known that the immunoregulatory cytokine interleukin (IL)-10 inhibits the accessory function of human dendritic cells (DC) in vitro. Recently, we have shown that these IL-10 DC inhibit the production of T helper cell 1 (Th1) and T helper cell 2 (Th2) cytokines by T cells from atopic individuals in vitro. The current study was set out to analyze whether IL-10 DC also exert inhibitory effects in vivo in a murine model of allergy to ovalbumin adsorbed to the adjuvant aluminium hydroxide (OVA/alum). Methods: OVA-pulsed or unpulsed bone marrow-derived DC, treated with IL-10 or left untreated during generation, were injected intravenously into BALB/c mice prior to and during OVA/alum sensitization, and sera and immune responses of mesenterial lymph node cells were analyzed. Additionally, bronchoalveolar lavage was performed after intranasal challenge with OVA. Results: Treatment of BALB/c mice with OVA-pulsed DC led to a significantly enhanced proliferation as well as Th2 (IL-4, IL-5), Th1 (interferon-γ) and IL-10 cytokine production after restimulation of lymph node cells with OVA in vitro compared with OVA immunization alone. In contrast, using OVA-pulsed IL-10 DC for transfer, proliferation and cytokine production by lymph node cells were not enhanced. OVA-specific immunoglobulin G1 (IgG1) and IgG2a production were significantly increased after transfer of OVA-pulsed DC and OVA-pulsed IL-10 DC, respectively, whereas anti-OVA IgE production and airway eosinophilia remained unchanged. Conclusions: Our data indicate that IL-10 treatment of DC decreases the Th1 and Th2 stimulatory capacity of DC but does not actually inhibit systemic (IgE) and local (airway inflammation) allergen-specific immune responses in a murine model of allergy.

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Paradoxical effects of interleukin‐10 on the maturation of murine myeloid dendritic cells

Cited by 26 publications

References 28 publications

Classical swine fever virus induces activation of plasmacytoid and conventional dendritic cells in tonsil, blood, and spleen of infected pigs

Classical swine fever virus induces activation of plasmacytoid and conventional dendritic cells in tonsil, blood, and spleen of infected pigs

Role of interleukin-10 in breast cancer

Interleukin-10-Treated Dendritic Cells Do Not Inhibit Th2 Immune Responses in Ovalbumin/Alum-Sensitized Mice

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