“Paraffin wax-overlay of pour plate”, a method for the isolation and enumeration of purple non-sulfur bacteria

Archana, A.; Sasikala, Ch.; Arunasri, Kotakonda

doi:10.1016/j.mimet.2004.08.006

Cited by 18 publications

(9 citation statements)

References 8 publications

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“…First, the organisms in the sludge sample were enriched with the modified RCVBN medium (Weaver et al 1975). Then the enriched sample was plated on a paraffin wax-overlay plate for providing an anaerobic setting (Archana et al 2004). The modified RCVBN medium plus 1.5% agar was used as the solid medium.…”

Section: Methodsmentioning

confidence: 99%

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ISOLATION AND CHARACTERIZATION OF CHLORELLA SOROKINIANA GXNN01 (CHLOROPHYTA) WITH THE PROPERTIES OF HETEROTROPHIC AND MICROAEROBIC GROWTH¹

Qiao

Wang

Zhang

2009

Journal of Phycology

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Heterotrophic and anaerobic microalgae are of significance in both basic research and industrial application. A microalga strain was isolated from a wastewater treatment pond and identified as Chlorella sorokiniana Shihira et W. R. Krauss GXNN01 in terms of morphology, physiology, and phylogeny. The strain grows rapidly in heterotrophic or mixotrophic conditions with addition of various carbon sources, and even in anaerobic conditions. The maximum growth rate reached 0.28 d(-1) when using d,l-malate as the carbon source, and the protein content of the microalgae was 75.32% in cell dry weight. The strain was shown to be capable of (1) utilizing d,l-malate only with light, (2) inhibiting photosynthesis in mixotrophic growth, and (3) growing in anaerobic conditions with regular photosynthesis and producing oxygen internally. This study demonstrates the influence of oxygen (aerobic vs. anaerobic) and metabolic regime (autotrophy, mixotrophy, heterotrophy) on the physiological state of the cell.

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Section: Methodsmentioning

confidence: 99%

“…1975). Then the enriched sample was plated on a paraffin wax‐overlay plate for providing an anaerobic setting (Archana et al. 2004).…”

mentioning

confidence: 99%

ISOLATION AND CHARACTERIZATION OF CHLORELLA SOROKINIANA GXNN01 (CHLOROPHYTA) WITH THE PROPERTIES OF HETEROTROPHIC AND MICROAEROBIC GROWTH¹

Qiao

Wang

Zhang

2009

Journal of Phycology

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“…They were grown on the modified Biebl and Pfennig's medium (Archana et al, 2004). The PNSB cultures were incubated under anaerobic conditions in the presence light (light intensity of 280~325 µE m -2 s -1 from fluorescent lamps) in 1 L screw…”

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confidence: 99%

Enhancement of growth and yield of tomato by Rhodopseudomonas sp. under greenhouse conditions

2008

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A greenhouse test was carried out to examine the effects on tomato growth of application of purple non-sulfur bacterium Rhodopseudomonas sp. which had enhanced germination and growth of tomato seed under axenic conditions. The shoot length of tomato plant inoculated by Rhodopseudomonas sp. KL9 increased by 34.6% compared to that of control in 8 weeks of cultivation. During the same period, this strain increased 120.6 and 78.6% of dry weight of shoot and root of tomato plants, respectively. The formation ratio of tomato fruit from flower was also raised by inoculation of KL9. In addition, Rhodopseudomonas sp. KL9 treatment enhanced the fresh weight and lycopene content in the harvested tomato fruits by 98.3 and 48.3%, respectively compared to those of the uninoculated control. When the effect on the indigenous bacterial community and fate of the inoculated Rhodopseudomonas sp. KL9 were monitored by denaturing gradient gel electrophoresis analysis, its application did not affect the native bacterial community in tomato rhizosphere soil, but should be repeated to maintain its population size. This bacterial capability may be applied as an environment-friendly biofertilizer to cultivation of high quality tomato and other crops including lycopene-containing vegetables and fruits.

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“…The hydrogen-producing photosynthetic bacterial strain was isolated using a method of anaerobic plating to maintain anaerobic conditions, as described by Archana et al [31]. Single colonies obtained on agar plates were re-streaked more than five times to ensure the purity of the culture.…”

Section: Strain Isolation and Identificationmentioning

confidence: 99%

Hydrogen production by a marine photosynthetic bacterium, Rhodovulum sulfidophilum P5, isolated from a shrimp pond

Cai

Wang

2012

International Journal of Hydrogen Energy

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“Paraffin wax-overlay of pour plate”, a method for the isolation and enumeration of purple non-sulfur bacteria

Cited by 18 publications

References 8 publications

ISOLATION AND CHARACTERIZATION OF CHLORELLA SOROKINIANA GXNN01 (CHLOROPHYTA) WITH THE PROPERTIES OF HETEROTROPHIC AND MICROAEROBIC GROWTH¹

ISOLATION AND CHARACTERIZATION OF CHLORELLA SOROKINIANA GXNN01 (CHLOROPHYTA) WITH THE PROPERTIES OF HETEROTROPHIC AND MICROAEROBIC GROWTH¹

Enhancement of growth and yield of tomato by Rhodopseudomonas sp. under greenhouse conditions

Hydrogen production by a marine photosynthetic bacterium, Rhodovulum sulfidophilum P5, isolated from a shrimp pond

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“Paraffin wax-overlay of pour plate”, a method for the isolation and enumeration of purple non-sulfur bacteria

Cited by 18 publications

References 8 publications

ISOLATION AND CHARACTERIZATION OF CHLORELLA SOROKINIANA GXNN01 (CHLOROPHYTA) WITH THE PROPERTIES OF HETEROTROPHIC AND MICROAEROBIC GROWTH1

ISOLATION AND CHARACTERIZATION OF CHLORELLA SOROKINIANA GXNN01 (CHLOROPHYTA) WITH THE PROPERTIES OF HETEROTROPHIC AND MICROAEROBIC GROWTH1

Enhancement of growth and yield of tomato by Rhodopseudomonas sp. under greenhouse conditions

Hydrogen production by a marine photosynthetic bacterium, Rhodovulum sulfidophilum P5, isolated from a shrimp pond

Contact Info

Product

Resources

About

ISOLATION AND CHARACTERIZATION OF CHLORELLA SOROKINIANA GXNN01 (CHLOROPHYTA) WITH THE PROPERTIES OF HETEROTROPHIC AND MICROAEROBIC GROWTH¹

ISOLATION AND CHARACTERIZATION OF CHLORELLA SOROKINIANA GXNN01 (CHLOROPHYTA) WITH THE PROPERTIES OF HETEROTROPHIC AND MICROAEROBIC GROWTH¹