In this research, we study an extended version of the joint order batching and scheduling optimization for manual vegetable order picking and packing lines with consideration of workers’ fatiguing effect. This problem is faced by many B2C fresh produce grocers in China on a daily basis which could severely decrease overall workflow efficiency in distribution center and customer satisfaction. In this order batching and sequencing problem, the setup time for processing each batch is volume-dependent and similarity dependent, as less ergonomic motion is needed in replenishing and picking similar orders. In addition, each worker’s fatiguing effect, usually caused by late shift and repetitive operation, which affects order processing times, is assumed to follow a general form of logistic growth with respect to the start time of order processing. We develop a heuristic approach to solve the resultant NP-hard problem for minimization of the total completion time. For order batching, a revised similarity index takes into account not only the number of common items in any two orders but also the proportion of these items based on the vegetable order feature. To sequence batches, the genetic algorithm is adapted and improved with proposed several efficient initialization and precedence rules. Within each batch, a revised nondecreasing item quantity algorithm is used. The performance of the proposed heuristic solution approach is evaluated using numerical instances generated from practical warehouse operations of our partnering B2C grocer. The efficiency of the proposed heuristic approach is demonstrated.