Parallel comparison of fibroblast-like synoviocytes from the surgically removed hyperplastic synovial tissues of rheumatoid arthritis and osteoarthritis patients

Abstract

BackgroundFibroblast-like synoviocytes (FLS) are essential cellular components in inflammatory joint diseases such as osteoarthritis (OA) and rheumatoid arthritis (RA). Despite the growing use of FLS isolated from OA and RA patients, a detailed functional and parallel comparison of FLS from these two types of arthritis has not been performed.MethodsIn the present study, FLS were isolated from surgically removed synovial tissues from twenty-two patients with OA and RA to evaluate their basic cellular functions.ResultsPure populations of FLS were isolated by a sorting strategy based on stringent marker expression (CD45−CD31−CD146−CD235a−CD90+PDPN+). OA FLS and RA FLS at the same passage (P2-P4) exhibited uniform fibroblast morphology. OA FLS and RA FLS expressed a similar profile of cell surface antigens, including the fibroblast markers VCAM1 and ICAM1. RA FLS showed a more sensitive inflammatory status than OA FLS with regard to proliferation, migration, apoptosis, inflammatory gene expression and pro-inflammatory cytokine secretion. In addition, the responses of OA FLS and RA FLS to both the pro-inflammatory cytokine tumor necrosis factor-alpha (TNF-α) and the anti-inflammatory drug methotrexate (MTX) were also evaluated here.ConclusionThe parallel comparison of OA FLS and RA FLS lays a foundation in preparation for when FLS are considered a potential therapeutic anti-inflammatory target for OA and RA.

Antioxidant Carbon Nanoparticles Inhibit Fibroblast-Like Synoviocyte Invasiveness and Reduce Disease Severity in a Rat Model of Rheumatoid Arthritis

“…However, healthy individuals do not undergo therapeutic joint surgery, it is therefore difficult to obtain healthy FLS. As control FLS, we and others [ 39 , 40 , 41 ] therefore use FLS from patients with osteoarthritis (OA) which are less aggressive than RA-FLS in terms of migration, invasiveness, and production of cytokine, chemokines and growth factors [ 42 , 43 ]. Concentrations of 10 μg/mL PEG-HCCs and higher induce the death of both RA-FLS and OA-FLS ( Figure 1 C).…”

“…Given that PEG-HCCs can enter FLS and reduce intracellular O 2 •− levels without affecting their survival at low doses, we next determined the effects of PEG-HCCs on FLS function. RA-FLS are more invasive and produce more pro-inflammatory cytokines and growth factors than do OA-FLS [ 42 ]. We therefore focused our analysis on the function of RA-FLS.…”

New Insights From Single-Cell Sequencing Data: Synovial Fibroblasts and Synovial Macrophages in Rheumatoid Arthritis

“…In addition, the proportion of CD34-THY1+ fibroblasts was correlated with the proportion of leukocyte infiltration, synovitis and synovial hypertrophy, which indicated that the altered proportion of fibroblast subsets in RA reflected the molecular level and clinical level of tissue inflammation. Huang et al (89) showed that some common fibroblast markers, consisting of procollagen I (COL1A1), Prolyl-4-hydroxylase (P-4-H), Vimentin, along with procollagen III (COL3A1), are different in OA FLS and RA FLS. Besides, RA FLS exhibits more severe cellular behavior in contrast with OA FLS, entailing a more rapid rate of proliferation, stronger invasive potential, and elevated expression as well as secretion of inflammatory cytokines.…”

Studies of non-autonomous effects of apoptosis in the course of in vitro apoptotic cell death initiation in healthy persons and patients with rheumatoid arthritis

“…Ревматоидный артрит (РА) -хроническое аутоиммунное заболевание, приводящее к поражению суставов и системным поражениям внутренних органов [17]. Известно, что РА характеризуется выраженным воспалительным статусом в отношении пролиферации, миграции, апоптоза, экспрессии генов и секреции провоспалительных цитокинов [23].…”

“…Это определяется типом клеток-мишеней, степенью их дифференцированности, а также особенностями функционирования внутриклеточных механизмов. Так, например, IL-4 и IL-10 ингибируют апоптоз Т-и В-лимфоцитов и индуцируют апоптоз циркулирующих моноцитов [23].…”