Parallel enzyme‐linked immunosorbent assay screening for human immunodeficiency virus among blood donors in five Chinese blood centres: a retrospective analysis

Zeng, Peibin; Liu, J.; Wang, J.; Dong, Xuejun; Li, J.; Bi, Xingang; Ma, Hongli; Wen, Xu; He, Mei; Liu, Y.; Ness, Paul M.; Shan, Hua; Study-II, Donor Evaluation

doi:10.1111/tme.12217

Cited by 4 publications

(2 citation statements)

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“…As a routine primary screening method for TTIs, EIA was widely used in clinical testing and blood station institutions [28]. However, EIA had high sensitivity and many interference factors, which cause false-positive reactions, such as sample addition, reaction temperature, reaction time, thoroughness of washing, hemolysis of specimens, and reagent factors.…”

Section: Discussionmentioning

confidence: 99%

Seroprevalence of Transfusion Transmissible Infections and Associated Risk Factors in Hospitalized Patients before Transfusion in Jinling Hospital Nanjing University: A Three-Year Retrospective Study

et al. 2022

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Transfusion-transmitted infections (TTIs), such as hepatitis B virus (HBV), hepatitis C virus (HCV), human immunodeficiency virus (HIV), and treponema pallidum (TP), must be detected before blood transfusion. However, few studies have been conducted on the prevalence and accuracy of positive results in hospitalized patients. The purpose of this study was to investigate the real seroprevalence of TTIs among patients before blood transfusion and analyze the characteristics of false-positive results in Jinling Hospital, Nanjing University, China. TTI results were collected from medical records and analyzed retrospectively. Additionally, we also used confirmatory assays to verify the accuracy of positive results. The overall prevalence of TTI was 8.96%, which was related to gender and age. The real positive rates were 86.67% (HBV), 35.09% (HCV), 20.75% (HIV), and 100% (TP). Our results also showed that high-speed centrifugation can reduce the false-positive rate of HBsAg. In summary, the results demonstrated that the positive rates of TTIs in hospitalized patients are higher than those in the general population. We also confirmed the existence of false-positive results in serological screening for TTIs. The method of processing specimens through high-speed centrifugation could reduce the false-positive results of detecting antigens effectively.

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Section: Discussionmentioning

confidence: 99%

Seroprevalence of Transfusion Transmissible Infections and Associated Risk Factors in Hospitalized Patients before Transfusion in Jinling Hospital Nanjing University: A Three-Year Retrospective Study

et al. 2022

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“…No analysis of donors could fail to mention infection. We plan to cover a series of topics related to TTIs next year, but in this issue Zeng et al () have reviewed parallel screening with different enzyme‐linked immunosorbent assay (ELISA) kits in the absence of nucleic acid testing (NAT) in nearly a million Chinese blood donors, Rouka et al () have described the epidemiology of Herpes viruses in Greek donors and Jarvis and a group from the Scottish Blood Service describe how Lyme borreliosis has increased considerably in Scotland, recently peaking at nearly 10 confirmed cases per 100 000 population and the serology of this infection in donors (Jarvis et al , ). Preventing recognised infection risk through the application of exclusion criteria is important.…”

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confidence: 99%