Parallel Epidemics of Community-Associated Methicillin-Resistant<i>Staphylococcus aureus</i>USA300 Infection in North and South America

Planet, Paul J.; Diaz, Lorena; Kolokotronis, Sergios‐Orestis; Narechania, Apurva; Reyes, Jinnethe; Xing, Galen; Rincón, Sandra; Smith, Howard W.; Panesso, Diana; Ryan, Chanelle; Smith, Dylan P.; Guzmán, Manuel; Zurita, Jeannete; Sebra, Robert; Deikus, Gintaras; Nolan, Robert L.; Tenover, Fred C.; Weinstock, George M.; Robinson, D. Ashley; Arias, César A.

doi:10.1093/infdis/jiv320

Cited by 115 publications

(206 citation statements)

References 46 publications

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“…The isolates recovered in Suriname carried the ACME element characteristic for the USA300-NA clade28. The isolates analyzed in this study also showed fluoroquinolone-resistance with the same mutations in grlA (S80Y) and gyrA (S84L) (Table 2) like those found in the USA300-NA clade, which has spread globally10.…”

Section: Discussionsupporting

confidence: 53%

“…Recent phylogenetic analyses28 revealed 2 dominant clades within the USA300 lineage that had a putative common ancestor in 1975 but segregated by geographical region, in North America (USA300 North American, USA300-NA) and in South America (USA300 Latin American variant, USA300-LV). The isolates recovered in Suriname carried the ACME element characteristic for the USA300-NA clade28.…”

Section: Discussionmentioning

confidence: 99%

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Complete-genome sequencing elucidates outbreak dynamics of CA-MRSA USA300 (ST8-spa t008) in an academic hospital of Paramaribo, Republic of Suriname

Sabat

Hermelijn

Akkerboom

et al. 2017

Sci Rep

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We report the investigation of an outbreak situation of methicillin-resistant Staphylococcus aureus (MRSA) that occurred at the Academic Hospital Paramaribo (AZP) in the Republic of Suriname from April to May 2013. We performed whole genome sequencing with complete gap closure for chromosomes and plasmids on all isolates. The outbreak involved 12 patients and 1 healthcare worker/nurse at the AZP. In total 24 isolates were investigated. spa typing, genome-wide single nucleotide polymorphism (SNP) analysis, ad hoc whole genome multilocus sequence typing (wgMLST), stable core genome MLST (cgMLST) and in silico PFGE were used to determine phylogenetic relatedness and to identify transmission. Whole-genome sequencing (WGS) showed that all isolates were members of genomic variants of the North American USA300 clone. However, WGS revealed a heterogeneous population structure of USA300 circulating at the AZP. We observed up to 8 SNPs or up to 5 alleles of difference by wgMLST when the isolates were recovered from different body sites of the same patient or if direct transmission between patients was most likely. This work describes the usefulness of complete genome sequencing of bacterial chromosomes and plasmids providing an unprecedented level of detail during outbreak investigations not being visible by using conventional typing methods.

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Section: Discussionsupporting

confidence: 53%

Section: Discussionmentioning

confidence: 99%

Complete-genome sequencing elucidates outbreak dynamics of CA-MRSA USA300 (ST8-spa t008) in an academic hospital of Paramaribo, Republic of Suriname

Sabat

Hermelijn

Akkerboom

et al. 2017

Sci Rep

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“…Methicillin‐resistant Staphylococcus aureus (MRSA) is widely distributed throughout the world, including mainland Ecuador (Planet et al, ). Originally, healthcare‐associated MRSA infections have emerged in the community and also from livestock in recent years (Stefani et al, ), becoming epidemic and causing severe disease and often fatal outcomes in humans (DeLeo & Chambers, ).…”

Section: Discussionmentioning

confidence: 99%

Antimicrobial resistance genes present in the faecal microbiota of free‐living Galapagos tortoises (Chelonoidis porteri)

Nieto-Claudín

Esperón

Blake

et al. 2019

Zoonoses and Public Health

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Antimicrobial resistance (AMR), encoded by plasmid‐mediated AMR genes (ARGs), is an increasing global public health threat. Wildlife play a fundamental role as sentinels, reservoirs and potential vectors of ARGs. For the first time in Galapagos, we have identified and quantified the presence of ARGs in free‐living giant tortoises (Chelonoidis porteri). We performed ARG analyses by quantitative PCR of faeces collected from the cloaca of 30 tortoises widely distributed across Santa Cruz Island. Validated samples (n = 28) were analysed by a panel of up to 21 different ARGs and all 28 tortoise samples were positive to one or more genes encoding resistance. Thirteen of 21 tested ARGs were present in at least one sample, and 10 tortoises (35.7%) had a multi‐resistant pattern. We recommend additional research so we may more fully understand resistance patterns across taxa and geographical locations throughout the Galapagos archipelago, and the implications of ARGs for the health of wildlife, domestic animals, and humans. In this study, we found 100% of sampled giant tortoises had ARGs present in their faeces, suggesting a large‐scale distribution of these genes within the archipelago.

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“…Genomic variants were mapped on the trees and correlated with phenotypic changes. For example, Planet and coworkers 39 worked on USA300 and USA300-LV clones, while Stinear and colleagues 40 on CA-MRSA ST93. Stegger et al 41 studied CC80 and Baines et al 42 focused their efforts on HA-MRSA ST239.…”

Section: Global Trends the Explosion Of Salmonella Typhi H58mentioning

confidence: 99%

Bacterial genomic epidemiology, from local outbreak characterization to species-history reconstruction

Gaiarsa

Marco

Comandatore

et al. 2015

Pathogens and Global Health

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Bacteriology has embraced the next-generation sequencing revolution, swiftly moving from the time of single genome sequencing to the age of genomic epidemiology. Hundreds and now even thousands of genomes are being sequenced for single bacterial species, allowing unprecedented levels of resolution and insight in the evolution and epidemic diffusion of the main bacterial pathogens. Here, we present a review of some of the most recent and groundbreaking studies in this field.

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Parallel Epidemics of Community-Associated Methicillin-ResistantStaphylococcus aureusUSA300 Infection in North and South America

Abstract: Our results reveal the existence of 2 parallel USA300 epidemics that shared a recent common ancestor. The simultaneous rapid dissemination of these 2 epidemic clades suggests the presence of shared, potentially convergent adaptations that enhance fitness and ability to spread.

Cited by 115 publications

References 46 publications

Complete-genome sequencing elucidates outbreak dynamics of CA-MRSA USA300 (ST8-spa t008) in an academic hospital of Paramaribo, Republic of Suriname

Complete-genome sequencing elucidates outbreak dynamics of CA-MRSA USA300 (ST8-spa t008) in an academic hospital of Paramaribo, Republic of Suriname

Antimicrobial resistance genes present in the faecal microbiota of free‐living Galapagos tortoises (Chelonoidis porteri)

Bacterial genomic epidemiology, from local outbreak characterization to species-history reconstruction

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