Parallel force measurement with a polymeric microbeam array using an optical microscope and micromanipulator

Sasoglu, F. Mert; Bohl, Andrew J.; Allen, Kathleen B.; Layton, Bradley E.

doi:10.1016/j.cmpb.2008.07.008

Cited by 5 publications

(2 citation statements)

References 34 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Parallelized stimulation with an array of microbeams actuated by positioning stages Sasoglu et al (2007Sasoglu et al ( , 2008) manufactured a comparable array of compliant microposts for stretching axons of multiple neurons aligned in a regular pattern. Pillars were, however, larger, with a diameter of 40 mm, a length of 120 mm.…”

Section: 2mentioning

confidence: 99%

Actuation means for the mechanical stimulation of living cells via microelectromechanical systems: A critical review

Desmaële

Boukallel

Régnier

2011

Journal of Biomechanics

View full text Add to dashboard Cite

Within a living body, cells are constantly exposed to various mechanical constraints. As a matter of fact, these mechanical factors play a vital role in the regulation of the cell state. It is widely recognized that cells can sense, react and adapt themselves to mechanical stimulation. However, investigations aimed at studying cell mechanics directly in vivo remain elusive. An alternative solution is to study cell mechanics via in vitro experiments. Nevertheless, this requires implementing means to mimic the stresses that cells naturally undergo in their physiological environment. In this paper, we survey various microelectromechanical systems (MEMS) dedicated to the mechanical stimulation of living cells. In particular, we focus on their actuation means as well as their inherent capabilities to stimulate a given amount of cells. Thereby, we report actuation means dependent upon the fact they can provide stimulation to a single cell, target a maximum of a hundred cells, or deal with thousands of cells. Intrinsic performances, strengths and limitations are summarized for each type of actuator. We also discuss recent achievements as well as future challenges of cell mechanostimulation.

show abstract

Section: 2mentioning

confidence: 99%

Actuation means for the mechanical stimulation of living cells via microelectromechanical systems: A critical review

Desmaële

Boukallel

Régnier

2011

Journal of Biomechanics

View full text Add to dashboard Cite

show abstract

“…However, micropipette aspiration can be challenging to apply to adherent cells. Sasoglu et al proposed a microfabrication technique to produce arrays of poly(dimethylsiloxane) (PDMS) cylindrical posts, which can be maneuvered by micromanipulators to simultaneously indent multiple adhered cells (11). However, to our knowledge, no parallel cell perturbation device has been demonstrated on live cells.…”

mentioning

confidence: 99%

Parallelized Cytoindentation Using Convex Micropatterned Surfaces

Jia¹,

Wee²,

Boudreau³

et al. 2016

BioTechniques

View full text Add to dashboard Cite

Here we present a high-throughput, parallelized cytoindentor for local compression of live cells. The cytoindentor uses convex lens-induced confinement (CLiC) to indent micrometer-sized areas in single cells and/or populations of cells with submicron precision. This is accomplished using micropatterned poly(dimethylsiloxane) (PDMS) films that are adhered to a convex lens to create arrays of extrusions referred to here as "posts." These posts caused local deformation of subcellular regions without any evidence of cell lysis upon CLiC indentation. Our micropost arrays were also functionalized with glycoproteins, such as fibronectin, to both pull and compress cells under customized confinement geometries. Measurements of Chinese hamster ovary (CHO-K1) cell migration trajectories and oxidative stress showed that the CLiC device did not damage or significantly stress the cells. Our novel tool opens a new area of investigation for visualizing mechanobiology and mechanochemistry within living cells, and the high-throughput nature of the technique will streamline investigations as current tools for mechanically probing material properties and molecular dynamics within cells, such as traditional cytoindentors and atomic force microscopy (AFM), are typically restricted to single-cell manipulation.

show abstract