Parallel origins of apomixis in two diverged evolutionary lineages in tribe Potentilleae (Rosaceae)

Dobeš, Christoph; Lückl, Andrea; Kausche, Lea; Scheffknecht, Susanne; Prohaska, Dagmar; Sykora, Christina; Paule, Juraj

doi:10.1111/boj.12239

Cited by 18 publications

(22 citation statements)

References 65 publications

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“…The genus Potentilla in its current taxonomic circumscription (Eriksson, Hibbs, Yoder, Delwiche, & Donoghue, 2003;Paule & Soják, 2009;Potter et al, 2007;Soják, 2008) expresses two principal modes of seed formation, regular sexuality and pseudogamous gametophytic apomixis (Dobeš et al, 2015). The origin of apomicts from sexual relatives is connected to polyploidization of the genome of apomicts.…”

Section: Discussionmentioning

confidence: 99%

Asymmetric reproductive interference: The consequences of cross‐pollination on reproductive success in sexual–apomictic populations of Potentilla puberula (Rosaceae)

Dobeš

Scheffknecht

Fenko

et al. 2017

Ecology and Evolution

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Apomixis evolves from a sexual background and usually is linked to polyploidization. Pseudogamous gametophytic apomicts, which require a fertilization to initiate seed development, of various ploidy levels frequently co‐occur with their lower‐ploid sexual ancestors, but the stability of such mixed populations is affected by reproductive interferences mediated by cross‐pollination. Thereby, reproductive success of crosses depends on the difference in ploidy levels of mating partners, that is, on tolerance of deviation from the balanced ratio of maternal versus paternal genomes. Quality of pollen can further affect reproductive success in intercytotype pollinations. Cross‐fertilization, however, can be avoided by selfing which may be induced upon pollination with mixtures of self‐ and cross‐pollen (i.e., mentor effects). We tested for reproductive compatibility of naturally co‐occurring tetraploid sexuals and penta‐ to octoploid apomicts in the rosaceous species Potentilla puberula by means of controlled crosses. We estimated the role of selfing as a crossing barrier and effects of self‐ and cross‐pollen quality as well as maternal: paternal genomic ratios in the endosperm on reproductive success. Cross‐fertilization of sexuals by apomicts was not blocked by selfing, and seed set was reduced in hetero‐ compared to homoploid crosses. Thereby, seed set was negatively related to deviations from balanced parental genomic ratios in the endosperm. In contrast, seed set in the apomictic cytotypes was not reduced in hetero‐ compared to homoploid crosses. Thus, apomictic cytotypes either avoided intercytotype cross‐fertilization through selfing, tolerated intercytotype cross‐fertilizations without negative effects on reproductive success, or even benefitted from higher pollen quality in intercytotype pollinations. Our experiment provides evidence for asymmetric reproductive interference, in favor of the apomicts, with significantly reduced seed set of sexuals in cytologically mixed populations, whereas seed set in apomicts was not affected. Incompleteness of crossing barriers further indicated at least partial losses of a parental genomic endosperm balance requirement.

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Section: Discussionmentioning

confidence: 99%

Asymmetric reproductive interference: The consequences of cross‐pollination on reproductive success in sexual–apomictic populations of Potentilla puberula (Rosaceae)

Dobeš

Scheffknecht

Fenko

et al. 2017

Ecology and Evolution

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“…Several seeds of both Onosma species showed larger P ind values, with the mean P ind = 3.22 for O. arenaria and 2.98 for O. pseudoarenaria. (42,(65)(66)(67)(68)). This is the first study evaluating the genome composition in ACAs and associating the genome composition with the FCSS results.…”

Section: Fcss Data Correspond To the Models Of Chromosome Inheritancementioning

confidence: 99%

“…Very high accuracy of FCSS measurements and the expected P ind values has previously been found for sexual or apomictic taxa in the cases when accurate FCSS data are reported (e.g., Refs. (42,(65)(66)(67)(68)).…”

Section: Fcss Data Correspond To the Models Of Chromosome Inheritancementioning

confidence: 99%

Flow cytometric seed screen data are consistent with models of chromosome inheritance in asymmetrically compensating allopolyploids

Kolarčik¹,

Kocová²,

Vašková³

2018

Cytometry Pt A

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Angiosperms have evolved a mechanism of double fertilization, which results in the production of a separate embryo (new individual) and endosperm (nutritive tissue). The flow cytometric seed screen (FCSS) was developed to infer plant reproduction modes based on endosperm-to-embryo DNA content ratio (P ). A ratio of 1.5 indicates sexual reproduction, whereas higher values of ≥2.0 are consistent with apomixis. Although FCSS has been successfully applied to the study of sexual and asexual plants, the limits of FCSS and particularly its potential for determination of reproduction modes in hemisexual plants have not been explored. Here, we evaluated the application of FCSS to the study of reproduction modes in two asymmetrically compensating allopolyploids (ACAs), Onosma arenaria and Rosa canina. These two species are characterized by the presence of asexually inherited univalent-forming and sexually inherited bivalent-forming chromosome sets. They both use asymmetric meiosis, which eliminates univalent-forming chromosome sets from the male gamete and retains them in the female gamete. Different chromosomal behavior in male and female meiosis in these plants is reflected in different theoretically derived P values, which deviate from a sexual 1.5 value. Here, we determined P FCSS-based values in seeds of ACAs, and compared the results to sexual species. As expected, we determined that the mean P is 1.51, 1.52, and 1.52 in the sexual plants, that is, Capsella bursa-pastoris, Crataegus monogyna, and O. pseudoarenaria, respectively. In the ACAs, different mean P values were determined for O. arenaria (1.61) and R. canina (1.82). These values are consistent with the theoretical P values determined based on models of chromosome inheritance. This study highlights the precision of flow cytometry in determining DNA content and it's utility in screening reproduction modes. Additionally, it advocates for more in-depth investigations into rapid screening of accessions where the P ratio has deviated from the 1.5 value typical of sexual species, which may indicate meiotic irregularities.

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“…Hybridization in the non-Maleae apomicts. -In Rosaceae subfamily Rosoideae the combination of gametophytic apomixis and polyploidy has been well known as an explanation for the taxonomic complexity of large genera like Alchemilla L. (summarized by Majeský & al., 2017) and Potentilla L. (tribe Potentilleae; Johri & al., 1992;Dobeš & al., 2015), and Rubus L. (tribe Rubeae; Kollmann & al., 2000). In Alchemilla, molecular phylogenetic studies (Gehrke & al., 2008) suggest that past hybridization is largely responsible for the incongruence between data from plastome and nrITS loci.…”

Section: Introductionmentioning

confidence: 99%

“…(Eriksen & Töpel, 2006;Feng & al., 2017). Gametophytic apomixis in Fragaria L. (also Potentilleae), however, appears to be evident only following hybridization (Asker & Jerling, 1992;Dziadczyk & al., 2011;Leszczuk & al., 2018), although even then evidence for its occurrence has been seen as equivocal (Nosrati & al., 2010;Dobeš & al., 2015). Confirmation of the hybrid status of several Fragaria species by Kamneva & al.…”

Section: Introductionmentioning

confidence: 99%

Sex and Rosaceae apomicts

Dickinson

2018

TAXON

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This paper updates earlier reviews of polyploidy and reproductive biology in the Rosaceae, and does so with a focus on hybridization in relation to polyploidy and (facultative) gametophytic apomixis. Taking data mainly from tribe Maleae, it also seeks to point out evidence for a potential role for fertilization of infrequent unreduced gametes in diploid-diploid crosses in producing autopolyploids. Apomixis may originate in these autopolyploids, and spread as they cross with diploids and other polyploids.

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Parallel origins of apomixis in two diverged evolutionary lineages in tribe Potentilleae (Rosaceae)

Cited by 18 publications

References 65 publications

Asymmetric reproductive interference: The consequences of cross‐pollination on reproductive success in sexual–apomictic populations of Potentilla puberula (Rosaceae)

Asymmetric reproductive interference: The consequences of cross‐pollination on reproductive success in sexual–apomictic populations of Potentilla puberula (Rosaceae)

Flow cytometric seed screen data are consistent with models of chromosome inheritance in asymmetrically compensating allopolyploids

Sex and Rosaceae apomicts

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