Parallel RNA extraction using magnetic beads and a droplet array

Shi, Xu; Chen, Chunhong; Gao, Weimin; Chao, Shih-Hui; Meldrum, Deirdre R.

doi:10.1039/c4lc01111b

Cited by 41 publications

(32 citation statements)

References 36 publications

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“…Shi et al used a similar strategy and created an array of traps to hold many droplets in position for parallel RNA extraction. 58 Another important application of surface modification is droplet dispensing. As mentioned above, droplet dispensing is virtually impossible using magnetic force alone.…”

Section: Assisted Magnetic Droplet Manipulationmentioning

confidence: 99%

“…These operations are relatively simple, as all the droplets travel along a single path. 53,58 To address more complexity and accomplish true multiplexing, individual droplets must be manipulated both separately and collectively. with surface wetting properties that are alterable by simple stretching.…”

Section: Droplet Manipulation For Multiplexingmentioning

confidence: 99%

“…56 Okochi and Shi both demonstrated parallel RNA extraction from multiple samples in a droplet array manipulated by magnetic particles. 57,58 Park et al demonstrated particle-based ELISA in parallel using a magnetic droplet array. 47 In most cases, the permanent magnet was positioned beneath the open surface.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Magnetic digital microfluidics – a review

2017

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A digital microfluidic platform manipulates droplets on an open surface. Magnetic digital microfluidics utilizes magnetic forces for actuation and offers unique advantages compared to other digital microfluidic platforms. First, the magnetic particles used in magnetic digital microfluidics have multiple functions. In addition to serving as actuators, they also provide a functional solid substrate for molecule binding, which enables a wide range of applications in molecular diagnostics and immunodiagnostics. Second, magnetic digital microfluidics can be manually operated in a "power-free" manner, which allows for operation in low-resource environments for point-of-care diagnostics where even batteries are considered a luxury item. This review covers research areas related to magnetic digital microfluidics. This paper first summarizes the current development of magnetic digital microfluidics. Various methods of droplet manipulation using magnetic forces are discussed, ranging from conventional magnetic particle-based actuation to the recent development of ferrofluids and magnetic liquid marbles. This paper also discusses several new approaches that use magnetically controlled flexible substrates for droplet manipulation. In addition, we emphasize applications of magnetic digital microfluidics in biosensing and medical diagnostics, and identify the current limitations of magnetic digital microfluidics. We provide a perspective on possible solutions to close these gaps. Finally, the paper discusses the future improvement of magnetic digital microfluidics to explore potential new research directions.

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Section: Assisted Magnetic Droplet Manipulationmentioning

confidence: 99%

Section: Droplet Manipulation For Multiplexingmentioning

confidence: 99%

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Magnetic digital microfluidics – a review

2017

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“…Thus, Shi et al presented a total RNA extraction droplet array to perform parallel RNA extractions on a droplet array. [127] Minemawari et al produced patterned organic semiconducting 2,7-dioctyl [1]benzothieno [3,2-b][1]benzothiophene (C 8 -BTBT) thin films of high crystallinity through antisolvent crystallization at the liquid-air interfaces of droplet microarrays, illustrating a way of producing surface transistor arrays [128] ( Figure 10A,B). Interestingly, the droplet geometries played an essential role for the crystallinity of the formed films.…”

Section: Microreactorsmentioning

confidence: 99%

Droplet Microarrays: From Surface Patterning to High‐Throughput Applications

2018

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High-throughput screening of live cells and chemical reactions in isolated droplets is an important and growing method in areas ranging from studies of gene functions and the search for new drug candidates, to performing combinatorial chemical reactions. Compared with microfluidics and well plates, the facile fabrication, high density, and open structure endow droplet microarrays on planar surfaces with great potential in the development of next-generation miniaturized platforms for high-throughput applications. Surfaces with special wettability have served as substrates to generate and/or address droplets microarrays. Here, the formation of droplet microarrays with designed geometry on chemically prepatterned surfaces is briefly described and some of the newer and emerging applications of these microarrays that are currently being explored are highlighted. Next, some of the available technologies used to add (bio-)chemical libraries to each droplet in parallel are introduced. Current challenges and future prospects that would benefit from using such droplet microarrays are also discussed.

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“…These buffers can be static or continuous. The movement of beads is controlled through the static buffers by the motion of the external permanent magnet by hand [2,19], while beads cross the continuous flow under the influence of the inhomogeneous magnetic field. Magnetic beads across various continuous flows has been applied in cell sorting [14,20,21], bioanalysis [15,22,23], capsule assembly [24], etc.…”

Section: Introductionmentioning

confidence: 99%

Continuous Microfluidic Purification of DNA Using Magnetophoresis

et al. 2020

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Automatic microfluidic purification of nucleic acid is predictable to reduce the input of original samples and improve the throughput of library preparation for sequencing. Here, we propose a novel microfluidic system using an external NdFeB magnet to isolate DNA from the polymerase chain reaction (PCR) mixture. The DNA was purified and isolated when the DNA-carrying beads transported to the interface of multi-laminar flow under the influence of magnetic field. Prior to the DNA recovery experiments, COMSOL simulations were carried out to study the relationship between trajectory of beads and magnet positions as well as fluid velocities. Afterwards, the experiments to study the influence of varying velocities and input of samples on the DNA recovery were conducted. Compared to experimental results, the relative error of the final position of beads is less than 10%. The recovery efficiency decreases with increase of input or fluid velocity, and the maximum DNA recovery efficiency is 98.4% with input of l00 ng DNA at fluid velocity of 1.373 mm/s. The results show that simulations significantly reduce the time for parameter adjustment in experiments. In addition, this platform uses a basic two-layer chip to realize automatic DNA isolation without any other liquid switch value or magnet controller.

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Parallel RNA extraction using magnetic beads and a droplet array

Abstract: Nucleic acid extraction is a necessary step for most genomic/transcriptomic analyses, but it often requires complicated mechanisms to be integrated into a lab-on-a-chip device.

Cited by 41 publications

References 36 publications

Magnetic digital microfluidics – a review

Magnetic digital microfluidics – a review

Droplet Microarrays: From Surface Patterning to High‐Throughput Applications

Continuous Microfluidic Purification of DNA Using Magnetophoresis

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