The pull-out method is a simple and effective method for detecting the preload of suspension bridge cable clamp bolts. However, research on the pull-out method is currently limited. The force principles governing the bolt during the pulling process are unclear, and the relationship between tension force and the desired preload remains uncertain. This paper aims to explore the force principles of bolts during the pull-out method detection process through a combined approach of theoretical analysis, full-scale test, and finite element simulation. The results indicate that the bolt preload increases during the pulling process. The preload detected by the pull-out method is not the initial preload of the bolt, but rather it exceeds the initial preload. The force relationships among various components are determined as follows: the preload subtracts the change value of the force exerted by the nut at the tension end, which equals the change value of tension force. Additionally, an analysis of the impact of the length of the bolt clamping section and the bolt area on the preload was conducted. Under the same bolt area, a shorter clamping section length corresponds to a greater increase in preload. With the same clamping section length, the increment of preload increases with the bolt area. These findings can serve as references for detecting and specifying the preload of the bolts.