Parameters governing gravitropic response of sporangiophores in Phycomyces blakesleeanus

Ootaki, Tamotsu; Ito, Kaoru; Abe, Mai; Lazarova, G.; Miyazaki, Akira; Tsuru, Toshisuke

doi:10.1007/bf02268600

Cited by 10 publications

(9 citation statements)

References 15 publications

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“…The white mutants that were used in this work lacked b-carotene (genotype car) and were derived from the wild type by mutagenesis with nitrosoguanidine. These mutants were: C2 carA2 geo-3 (A) (Bergman et al 1973;Schimek et al 1998); C5 carB10 geo-10 (A), a hypergravitropic mutant (Meissner and DelbruÈ ck 1968;Ootaki et al 1995); C171 carA30 carR21 (A); C213 carA5 geo-3 (A) (Schimek et al 1998); C148 carA5 madC geo-3 (A) (Bergman et al 1973); A453 carA5 madB106 (A) (provided by A.P. Eslava, Salamanca, Spain); and L5 carA701 madA7 (A) (provided by E.D.…”

Section: Methodsmentioning

confidence: 99%

Light-induced absorbance changes in Phycomyces : evidence for cryptochrome-associated flavosemiquinones

Schmidt

Galland

1999

Planta

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Light-induced absorbance changes (LIACs), which are associated with early photochemical events of blue-light transduction, were detected in growing zones of Phycomyces sporangiophores. The novel LIACs meet all the essential requirements for a spectrophotometric photoreceptor assay which was previously unavailable for blue-light receptors (cryptochromes). In-vivo absorption spectra of growing zones were derived from reflection spectra which were measured with a novel rapid-scan spectrophotometer. To detect photoreceptor-associated absorbance changes white mutants were employed which lack the interfering bulk pigment beta-carotene. Blue and white light, not however red light, induced in these strains absorbance changes near 460-490 and 600-620 nm. The LIACs were absent in light-insensitive mutants with defects in the genes madA, madB and madC. Because these genes affect photosensory adaptation and the blue-light receptor itself, the novel in-vivo LIACs must be associated with photochemical events which occur early in the transduction chain. The spectral characteristics of the LIACs are in accordance with a blue- and red-light absorbing flavosemiquinone which is generated upon light absorption by an oxidized flavin receptor. It is proposed that the flavosemiquinone functions itself as photoreceptor which mediates several red-light responses of Phycomyces.

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Section: Methodsmentioning

confidence: 99%

Light-induced absorbance changes in Phycomyces : evidence for cryptochrome-associated flavosemiquinones

Schmidt

Galland

1999

Planta

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“…For the wild type we found about 540 crystals per growing zone and for mutants C5 and A909 280 and 700 crystals, respectively ( Table 2). The rather low crystal number of the hypergravitropic strain C5 may be due to the fact that this mutant has sporangiophores which are thinner than those of the wild-type strain (Ootaki et al, 1995). The diameter of C5 is about 70% of that of the wild type.…”

Section: Size Distribution and Density Of Octahedral Crystalsmentioning

confidence: 96%

Statoliths in Phycomyces: Characterization of Octahedral Protein Crystals

Eibel

Schimek

Fries

et al. 2000

Fungal Genetics and Biology

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“…5), whereas phototropism required 3 h (Page, 1962). This is also the case in Phycomyces, which required about 20h until the gravitropic bending reaches its maximum, but 10 h is sufficient for phototropism (Ootaki et al, 1995). The reasons for these differences remain unsolved.…”

Section: +-Omentioning

confidence: 95%

Developmental stage-dependent response of Pilobolus crystallinus sporangiophores to gravitative and centrifugal stimulation

et al. 1998

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Gravitropic response of sporangiophores of Pilobolus crystallinus was studied by successive microscopic observation of the sporangiophores horizontally placed in the dark (red light) and by analysis of sporangiophore response to centrifugal stimulation. Negative tropism against the gravitative and also centrifugal stimulation was found only in mature sporangiophores after development of sporangium and after the resumption of elongation beneath the fully-developed subsporangial vesicle, but there was no response in younger sporangiophores, implying that the gravitative perception system of the sporangiophores is dependent on their developmental stages.

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Parameters governing gravitropic response of sporangiophores in Phycomyces blakesleeanus

Cited by 10 publications

References 15 publications

Light-induced absorbance changes in Phycomyces : evidence for cryptochrome-associated flavosemiquinones

Light-induced absorbance changes in Phycomyces : evidence for cryptochrome-associated flavosemiquinones

Statoliths in Phycomyces: Characterization of Octahedral Protein Crystals

Developmental stage-dependent response of Pilobolus crystallinus sporangiophores to gravitative and centrifugal stimulation

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