A study was conducted to determine the impact of crating density and transportation distance on losses and physiological response of broilers during the winter season. For this, ROSS-308 broilers were crated at three densities (i.e., 10, 12, and 15 birds/crate) in plastic crates and transported in three distances (i.e., 80, 160, and 240 km) during the winter season at 3.6-9.5ºC temperature and 63.3-78.8% relative humidity. Results showed that body weight loss increased significantly with the increase in transportation distance and decrease in crating density, whereas dead on arrival, physical injuries and bruises were not different among different transportation distance and crating density treatments. Significant reduction in carcass and breast yield was observed with the increase in transportation distance, whereas an increase in crating density above 12 birds per crate increased breast yield. Birds transported for 240 km had the highest serum catalase activity and the lowest rectal body temperature compared to other treatments. A gradual increase in crating density resulted in the increase in thyroxine concentration and reduction in rectal temperature. Serum glucose, albumin and uric acid concertation increased significantly after 160km of transportation, but serum triglyceride contents decreased. Moreover, an increase in crating density also increased serum glucose and triglyceride. Results suggested that longer transportation distance during the winter season increased body weight shrinkage with a reduction in carcass and breast yield, and the impact of losses and stress was greater in treatments having a lower number of birds in crates.