Parametrial Adipose Tissue and Metabolic Dysfunctions Induced by Fructose‐rich Diet in Normal and Neonatal‐androgenized Adult Female Rats

Alzamendi, Ana; Castrogiovanni, Daniel; Ortega, Hugo Héctor; Gaillard, Rolf C.; Giovambattista, Andrés; Spinedi, Eduardo

doi:10.1038/oby.2009.255

Cited by 24 publications

(84 citation statements)

References 42 publications

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“…obesity; litter size; leptin; fat mass NUMEROUS STUDIES HAVE SHOWN that an individual's propensity to gain weight during adulthood can be affected by prenatal and neonatal changes in nutrition (23,42,57). Moreover, it is now clear that it is not only the amount but also the type of energy consumed that determines weight gain and body composition during both early development and adulthood (1,23,40,54). Thus, poor nutrition and overnutrition during maturational periods and adulthood are likely to synergize in causation of the exponential rise in obesity observed in many populations.…”

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confidence: 97%

Early postnatal overnutrition increases adipose tissue accrual in response to a sucrose-enriched diet

Fuente-Martín

García‐Cáceres

Granado

et al. 2012

American Journal of Physiology-Endocrinology and Metabolism

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Both overnutrition and an incorrect nutrient balance have contributed to the rise in obesity. Moreover, it is now clear that poor nutrition during early life augments the possibility of excess weight gain in later years. Our aim was to determine how neonatal overnutrition affects later responses to a sucrose-enriched diet and whether this varies depending upon when the diet is introduced in postnatal life. Male Wistar rats raised in litters of four or 12 pups were given a 33% sucrose solution instead of water from weaning (day 21) or postnatal day (PND) 65. All rats received normal chow ad libitum until they were euthanized on PND 80. Body weight (BW) and food and liquid intake were monitored throughout the study. Fat mass, adipocyte morphology, serum biochemical and hormonal parameters, and hypothalamic neuropeptide mRNA levels were measured at study termination. Neonatal overnutrition increased food intake, BW, and leptin levels, induced adipocyte hypertrophy, and decreased total ghrelin levels. The sucrose-enriched diet increased total energy intake, adipose accrual, and leptin, adiponectin, and acylated ghrelin levels but decreased BW. Most of these responses were accentuated in neonatally overnourished rats, which also had increased insulin and triglyceride levels. However, long-term sucrose intake induced adipocyte hypertrophy in rats from normal-sized litters but not in neonatally overfed rats. The results reported here indicate that neonatal overnutrition increases the detrimental response to a diet rich in sucrose later in life. Moreover, the timing and duration of the exposure to a sucrose-enriched diet alter the adverse metabolic outcomes.

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mentioning

confidence: 97%

Early postnatal overnutrition increases adipose tissue accrual in response to a sucrose-enriched diet

Fuente-Martín

García‐Cáceres

Granado

et al. 2012

American Journal of Physiology-Endocrinology and Metabolism

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“…the unhealthy expansion) of its cells [6] and an increased peripheral/VAT oxidative stress [3]. However, the underlying mechanism whereby the FRD induces this selective adipocyte hypertrophy rather than its hyperplasia remains unknown.…”

Section: Introductionmentioning

confidence: 99%

Excess fructose intake‐induced hypertrophic visceral adipose tissue results from unbalanced precursor cell adipogenic signals

et al. 2013

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We studied the effect of feeding normal adult male rats with a commercial diet supplemented with fructose added to the drinking water (10% w/v; fructose-rich diet, FRD) on the adipogenic capacity of stromal-vascular fraction (SVF) cells isolated from visceral adipose tissue (VAT) pads. Animals received either the commercial diet or FRD ad libitum for 3 weeks; thereafter, we evaluated the in vitro proliferative and adipogenic capacities of their VAT SVF cells. FRD significantly increased plasma insulin, triglyceride and leptin levels, VAT mass/cell size, and the in vitro adipogenic capacity of SVF cells. Flow cytometry studies indicated that the VAT precursor cell population number did not differ between groups; however, the accelerated adipogenic process could result from an imbalance between endogenous pro-and anti-adipogenic SVF cell signals, which are clearly shifted towards the former. The increased insulin milieu and its intracellular mediator (insulin receptor substrate-1) in VAT pads, as well as the enhanced SVF cell expression of Zpf423 and peroxisome proliferator receptor-c2 (all pro-adipogenic modulators), together with a decreased SVF cell concentration of anti-adipogenic factors (pre-adipocyte factor-1 and wingless-type MMTV-10b), strongly supports this assumption. We hypothesize that the VAT mass expansion recorded in FRD rats results from the combination of initial accelerated adipogenesis and final cell hypertrophy. It remains to be determined whether FRD administration over longer periods could perpetuate both processes, or whether cell hypertrophy itself remains responsible for a further VAT mass expansion, as observed in advanced/morbid obesity.

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“…Regarding the low leptin levels found in FTM rats, it has been reported that in the adult neonatally androgenized female rat [48] and in the normal male rat over-development [49] , the increase in plasma leptin levels seems to be related, at least in part, to an androgen effect. It has been claimed that there is a proinflammatory role of leptin [23] , which could contradict our data on LPS-stimulated TNF ␣ secretion in FTM rats.…”

Section: Discussionmentioning

confidence: 99%

Enhanced Proinflammatory Cytokine Response to Bacterial Lipopolysaccharide in the Adult Male Rat after either Neonatal or Prepubertal Ablation of Biological Testosterone Activity

Ongaro

Castrogiovanni²,

Giovambattista³

et al. 2011

Neuroimmunomodulation

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A sex steroid-dependent modulation of the immune function in mammals is accepted, and evidence suggests that while estrogens enhance, androgens inhibit the immune response. The aim of this study was to explore in the adult male rat the effect of either neonatal flutamide (FTM) treatment or prepubertal orchidectomy (ODX) on endocrine markers in the basal condition and peripheral tumor necrosis factor alpha (TNFα) levels during inflammatory stress. For these purposes, (1) 5-day-old male rats were subcutaneously injected with either sterile vehicle alone or containing 1.75 mg FTM, and (2) 25-day-old male rats were sham operated or had ODX. Rats were sacrificed (at 100 days of age) in the basal condition for determination of peripheral metabolite levels. Additional rats were intravenously injected with bacterial lipopolysaccharide (LPS; 25 µg/kg body weight, i.v.) and bled for up to 4 h. Data indicate that (1) ODX increased peripheral glucocorticoid levels and reduced those of testosterone, whereas FTM-treated rats displayed low circulating leptin concentrations, and (2) LPS-induced TNFα secretion in plasma was significantly enhanced in the FTM and ODX groups. Our study supports that neonatal FTM treatment affected adiposity function, and adds data maintaining that androgens have a suppressive role in proinflammatory cytokine release in plasma during inflammation.

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Parametrial Adipose Tissue and Metabolic Dysfunctions Induced by Fructose‐rich Diet in Normal and Neonatal‐androgenized Adult Female Rats

Cited by 24 publications

References 42 publications

Early postnatal overnutrition increases adipose tissue accrual in response to a sucrose-enriched diet

Early postnatal overnutrition increases adipose tissue accrual in response to a sucrose-enriched diet

Excess fructose intake‐induced hypertrophic visceral adipose tissue results from unbalanced precursor cell adipogenic signals

Enhanced Proinflammatory Cytokine Response to Bacterial Lipopolysaccharide in the Adult Male Rat after either Neonatal or Prepubertal Ablation of Biological Testosterone Activity

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