As a new flexible supporting structure, prestressed anchor cable lattice beams have been widely used in high-slope support engineering and have achieved good results. However, theoretical research on the internal force analysis of lattice beams is far behind engineering practice. Based on the theory of the Winkler elastic foundation model, a mechanical model of a prestressed anchor cable lattice beam at the tension stage was established. Considering the nonhomogeneous lattice beam materials, a calculation method was given and applied to engineering examples. A calculation method of the measured moment was introduced in the field test conducted in the Zhouqu County “8·8” debris flow disaster reconstruction project. Comparisons between the test results and the theoretical results were performed. The results showed that the theoretical results of the distribution trend of the lattice beam moment were consistent with the test results, which verified the rationality of the proposed calculation method. The inertia moment of the beam section solved by the transformed section method was more realistic. The results of the transformed section method could improve the bending resistance of the lattice beam and reduce the reinforcement ratio. The greater the anchoring force was, the more obvious the lifting effect was. The anchoring force was an important influencing factor of the internal force of the lattice beam. The greater the anchoring force was, the greater the lattice beam moment was, and they showed the same proportional change phenomenon. Compared with the theoretical moment, the measured moment obtained by the test was smaller, which indicated that the lattice beam of the tested slope was safe at the present stage.