Parametric study of a microdialysis probe and study of depletion effect using ethanol as a test analyte

Lee, Tse-Ang; Gonzales, Rueben A.; Hutter, Tanya

doi:10.1016/j.bbrc.2022.10.086

Cited by 4 publications

(6 citation statements)

References 24 publications

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“…Figure 1 shows the diagram of the in vitro microdialysis setup. Two pieces of fused silica tubing were used for the inlet and outlet lines of the probe as previously described 26 . For this study, probes with a 2 mm working distance of the hollow fiber membrane were fabricated.…”

Section: Methodsmentioning

confidence: 99%

“…Two pieces of fused silica tubing were used for the inlet and outlet lines of the probe as previously described. 26 For this study, probes with a 2 mm working distance of the hollow fiber membrane were fabricated. The probe was dipped into the solution through a hole drilled into the cap, which was sealed to minimize evaporation.…”

Section: In Vitro Microdialysis Experimentsmentioning

confidence: 99%

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Experimental investigation of regenerated cellulose microdialysis probe sterilization

Bhaduri,

Lee,

Peng

et al. 2024

J of Applied Polymer Sci

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Sterilization of devices is important in hospitals, operating theaters, and emergency rooms. Microdialysis allows in vivo sampling of small molecules and is used for clinical studies. Microdialysis probes are made of soft, flexible, and porous polymeric membranes. They have been traditionally disinfected using either ethanol (which fails to eliminate all microbes and does not satisfy regulatory requirements) or ethylene oxide gas and gamma irradiation (that are expensive and resource‐intensive). In this work, three methods for microdialysis probe‐sterilization were studied—autoclave and two chemicals (commercially available sterilization solutions): Sporox II and MetriCide. Following sterilization, the regenerated cellulose membranes were characterized under scanning electron microscopy and by measuring the changes in pore characteristics using nitrogen sorption. To determine the effect of sterilization on analyte diffusion through the membrane, microdialysis probes were fabricated, sterilized, and tested with two analytes; ethanol and dopamine. The autoclaved membranes suffered thermomechanical damage and were deemed unfit for further testing. Probes sterilized with the chemical solutions were subsequently characterized by in vitro microdialysis experiments performed under regulated mass flux conditions. It is concluded that autoclaving is not a suitable sterilization technique for the cellulose membranes, while both of the chemical sterilizers were found to be good candidates for sterilization.

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Section: Methodsmentioning

confidence: 99%

Section: In Vitro Microdialysis Experimentsmentioning

confidence: 99%

Experimental investigation of regenerated cellulose microdialysis probe sterilization

Bhaduri,

Lee,

Peng

et al. 2024

J of Applied Polymer Sci

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“…Lab-made probes were fabricated using the same method described in a previous paper, which employed a side-by-side configuration. 34 In brief, two pieces of fused silica tubing were used for the inlet and outlet lines of the probe. Both pieces of tubing were inserted into a piece of regenerated cellulose membrane (Spectra/Por®) with a molecular weight cutoff (MWCO) of 13 kDa.…”

Section: Methodsmentioning

confidence: 99%

“…The setup used in this research is similar to a previously published work. 34 In short, a 100 mL media bottle was used to contain different compounds prepared in aCSF to simulate the brain environment after ethanol administration and investigate the performance of the probe. The probe was dipped into the solution through a hole drilled on the cap which was sealed to minimize evaporation of the standard solution.…”

Section: Methodsmentioning

confidence: 99%

Experimental and numerical investigation of microdialysis probes for ethanol metabolism studies

Lee,

Peng,

Walia

et al. 2024

Anal. Methods

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“…Microdialysis probes were fabricated using the same method as described in detail in our previous paper 7 . In short, the inlet and outlet fused silica tubing were inserted into a piece of regenerated cellulose membrane with 13 kDa molecular weight cut-off.…”

Section: Microdialysis Probe Fabricationmentioning

confidence: 99%

Infrared spectroscopy for neurochemical monitoring of alcohol and its metabolites

Lee¹,

Peng²,

Kowal³

et al. 2023

Frontiers in Biological Detection: From Nanosensors to Systems XV

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Continuous measurement of the concentrations of ethanol and its metabolites, acetaldehyde and acetic acid, in vivo is important for the alcohol research community. Most studies only measure ethanol because accurate measurement of all three compounds is challenging. Measurement inside tissue/brain is done using a microdialysis technique, followed by off-line analysis using gas chromatography (GC). To realize simultaneous measurement of ethanol and its metabolites, one can take advantage of infrared (IR) spectroscopy as a rapid and reagent-free method. Here we report a feasibility study of using IR spectroscopy to simultaneously measure ethanol, acetaldehyde and acetic acid in aqueous solution. Different concentrations in transmission mode at different optical pathlengths and using attenuated total reflectance (ATR) were measured. In vitro microdialysis was performed on the mixture of the three compounds, and the collected sample was measured using IR to demonstrate the capability of quantifying the concentrations of the three analytes simultaneously. Lastly, to overcome the limitations of the microdialysis technique, direct measurement using evanescent-field IR spectroscopy can be a potential alternative. A hydrophobic polymer coating that adsorbs ethanol and excludes water, could improve sensitivity. Sorption kinetics in polymethyl methacrylate (PMMA) and polydimethylsiloxane (PDMS) coatings on an ATR crystal were measured. Both polymers demonstrate preferential adsorption of ethanol over water.

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Parametric study of a microdialysis probe and study of depletion effect using ethanol as a test analyte

Cited by 4 publications

References 24 publications

Experimental investigation of regenerated cellulose microdialysis probe sterilization

Experimental investigation of regenerated cellulose microdialysis probe sterilization

Experimental and numerical investigation of microdialysis probes for ethanol metabolism studies

Infrared spectroscopy for neurochemical monitoring of alcohol and its metabolites

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