Parametrization of the deformation curve as a tool for standardization and interpretation of ektacytometric measurements

Bronkhorst, P.J.H.; Nijhof, Evert-Jan; Sixma, Jan J.

doi:10.3233/ch-1995-15602

Cited by 6 publications

(2 citation statements)

References 24 publications

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“…Therefore, the LB equation is a special form of the Streekstra equation (Equation 4) in which m = 1. The Streekstra model was modified by Bronkhorst et al [4] to yield the Streekstra-Bronkhorst (SB) equation in which an EI min term was added that allows EI to have negative values at low SS:…”

Section: Summary Of Approaches Proposed For Data Reduction In Ektacytmentioning

confidence: 99%

“…A single index reflecting RBC deformability would be useful, in experimental and clinical studies [7,12,14,15,22]. Various approaches have been suggested to derive indices of RBC deformability based on EI-SS curves [4,6,7,19], some being implemented into software programs that are part of commercial instruments (e.g., LORCA). These approaches were critically analyzed by various groups and recommendations for their use have been published [2,3,11].…”

Section: Introductionmentioning

confidence: 99%

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Data reduction methods for ektacytometry in clinical hemorheology

Başkurt

Meiselman

2013

Clinical Hemorheology and Microcirculation

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Laser-diffraction ektacytometry is a generally accepted technique for measuring RBC deformability induced by fluid shear stress (SS) and yields paired elongation index-SS data at several levels of stress. Unfortunately, comparison of results is hindered by the lack of simple indices that accurately characterize these data. Several mathematical models have been proposed, including those developed for analysis of enzyme kinetics (Lineweaver-Burk, Eadie-Hofstee) and curve fitting (Streekstra-Bronkhorst). All of these analytical approaches provide a value for cell deformation at infinite stress (EI max ) and the shear stress required to achieve one-half of this deformation (SS 1/2 ); the use of non-linear regression is essential when calculating these parameters. While the current models provide equivalent results for normal RBC if used with non-linear regression, EI max and SS 1/2 are not always concordant for cells with abnormal mechanical behavior. This technical note examines such differences for three conditions: glutaraldehyde treatment, mechanical stress and non-isotonic media. It was found that none of the models yield completely satisfactory values for EI max and SS 1/2 , especially if there are large changes of EI max . However, the ratio of SS 1/2 to EI max (SS 1/2 /EI max ) is much less affected by these problems, has similar power (i.e., standardized difference) as SS 1/2 and EI max and is more robust in reflecting alterations of deformability. We thus conclude that the SS 1/2 /EI max ratio can be used when reporting and comparing various populations of RBC or cells obtained from subjects having different clinical states.

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Section: Summary Of Approaches Proposed For Data Reduction In Ektacytmentioning

confidence: 99%