The aim of this study was to evaluate the effect of different dietary electrolyte balance (DEB) levels over the hematological parameters and the biochemical profile of broilers under stress caused by cyclic heat. We used 1050 male broilers from Ross lineage, for two experimental phases: phase 1 (broilers from 1 until 21 days old) and phase 2 (broilers from 22 until 42 days old). The broilers were distributed under a completely randomized design, built for five treatments (110; 175; 240; 305; 370 mEq of DEB/kg of ration) and seven repetitions. For the hematological analyses were used two broilers in each repetition, one to blood count evaluation (Hemogram) and another one to the biochemistry evaluation, collected in the end of each experimental phase. During the phase of 1 - 21 days old, the variable hemoglobin number (Heme), mean corpuscular volume (MCV) and Serum Chloride (Cl) presented a linear effect (p<0,05) DEB levels. There was no effects (p>0,05) over the corpuscular volume (CV), total plasma protein (TPP), leukocytes number (Leu), heterophiles (Het), lymphocytes (Lyn), eosinophils (Eos), basophils (Bas), monocytes (Mon), calcium (Ca), phosphor (P), uric acid (UA) e urea (U). During the phase two of 22 – 42 days old there was effect (p<0,05) quadratic to the variables Leu and Het and linear to the variable P and significative difference (p<0,05) between the variable means UA at the levels of DEB tested.There was no effect (p>0,05) over the variable CV, Heme, MCV, TPP, Lyn, Eos, Bas, Mon, Cl, Ca and U on the DEB levels tested. The supplementation of the DEB levels 110, 175 e 240, 305 and 370 to the phase from 1 until 21 days old presented great results in the hematological evaluation and biochemical, because it provided possibly a longer useful life to the Hem without promoting a metabolic imbalance. In the phase from 22 until 42 days old the level of 268 to 280 mEq of DEB/kg of ration presented a better answer from the broilers immune systemic.