Paramyxoviridae: Mumps Virus

Örvell, Claes

doi:10.1007/978-1-4612-3900-0_26

Laboratory Diagnosis of Infectious Diseases Principles and Practice 1988

DOI: 10.1007/978-1-4612-3900-0_26

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Paramyxoviridae: Mumps Virus

Claes Örvell¹

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Cited by 2 publications

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“…Hamster sera were also tested for MuV neutralizing activity. Neutralization assays were performed as described previously (Orvell, 1988). The results shown in Table 1 demonstrated, in the absence of complement, a neutralizing activity in the Hanti F 3205 sera and, although to a lower extent, in the Hanti H N 3208 sera.…”

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confidence: 92%

Protection of hamsters against experimental mumps virus (MuV) infection by antibodies raised against the MuV surface glycoproteins expressed from recombinant vaccinia virus vectors

Houard¹,

Varsànyi

Milican³

et al. 1995

Journal of General Virology

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The fusion (F) and haemagglutinin-neuraminidase (HN) glycoproteins of mumps virus (MuV) have been produced in CV1 cells via vaccinia virus recombinants.Recombinant proteins accumulated in infected cells and were glycosylated. Upon reduction, the F protein product was completely converted into its subunits.Hamsters infected with vaccinia recombinants expressing either the F or HN proteins produced antibodies recognizing MuV antigens and neutralizing MuV infectivity in vitro. These antibodies provided protection against MuV-induced encephalitis in newborn hamsters.

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mentioning

confidence: 92%

Protection of hamsters against experimental mumps virus (MuV) infection by antibodies raised against the MuV surface glycoproteins expressed from recombinant vaccinia virus vectors

Houard¹,

Varsànyi

Milican³

et al. 1995

Journal of General Virology

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Transport of viral specimens

Johnson

1990

Clin Microbiol Rev

102

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The diagnosis of viral infections by culture relies on the collection of proper specimens, proper care to protect the virus in the specimens from environmental damage, and use of an adequate transport system to maintain virus activity. Collection of specimens with swabs that are toxic to either virus or cell culture should be avoided. A variety of transport media have been formulated, beginning with early bacteriological transport media. Certain swab-tube combinations have proven to be both effective and convenient. Of the liquid transport media, sucrose-based and broth-based media appear to be the most widely accepted and used. Studies on virus stability show that most viruses tested are sufficiently stable in transport media to withstand a transport time of 1 to 3 days. Some viruses may withstand longer transport times. In many cases, it is not necessary to store virus specimens in a refrigerator or send them to the laboratory on wet ice or frozen on dry ice. However, the specimen should not be exposed to environmental extremes. Modern viral transport media allow for more effective use of viral culture and culture enhancement techniques for the diagnosis of human viral infections.

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Paramyxoviridae: Mumps Virus

Cited by 2 publications

References 78 publications

Protection of hamsters against experimental mumps virus (MuV) infection by antibodies raised against the MuV surface glycoproteins expressed from recombinant vaccinia virus vectors

Protection of hamsters against experimental mumps virus (MuV) infection by antibodies raised against the MuV surface glycoproteins expressed from recombinant vaccinia virus vectors

Transport of viral specimens

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